Abstract: Provided is a method for detecting a microRNA. The method comprises: adding both (a) a polyadenylic acid tailing reaction system and (b) a reverse transcription reaction system to a sample to be detected, and subjecting the mixture to a one-step method by means of using a universal reverse transcription primer to obtain a reverse-transcribed cDNA product; and amplifying the product with a downstream universal primer and a specific upstream primer and detecting the amplified product with a universal fluorescence probe in a DNA amplification reaction and fluorescence detection system. Further provided is a kit based on the above-mentioned method. The provided method and kit have significantly higher sensitivity and specificity than traditional methods, can realize high-throughput sensitive detection with a small amount of samples, are convenient to operate and consume a short amount of time, and can detect batches of target microRNAs at low cost.

Abstract: Methods for identifying or classifying a gene mutation type associated with acquired drug resistance of cancer is provided. Said methods may include determining a total copy number (N) of a susceptible gene in a cancer cell, identifying a mutant copy number of the susceptible gene, determining a mutant copy number sufficient to cause acquired drug resistance (M); and comparing N with M to identify or classify the mutation type in the cancer cell.