Abstract: Patch (1) for regenerating biological tissues in patients and method of manufacture associated with the same, comprising a collagen membrane (2) which comprises cells with a regenerative character; and at least one reinforcement element (3a, 3b) configured to increase the rigidity of said membrane (2), preventing the deformation of said membrane (2) during the handling thereof; wherein said reinforcement element (3a, 3b) can be a perimeter frame (3a) and/or a crossed attachment (3b) between the ends of said membrane (2); which provides a patch (1) which can be applied to any type of biological organ with maximum assurances of success as well as reducing the application time.