Abstract: Methods, systems, and kits for generating placenta-like organoids in suspension or placenta-on-a-chip systems are described herein, as well as methods of use thereof. A placental organoid composition has a plurality of trophoblast cells derived from naïve-like stem cells in suspension cultures. The placental organoid composition can be prepared by seeding a single-cell suspension onto a culture surface in a first medium, centrifuging and incubating the single-cell suspension to form aggregates. A portion of the first media is replaced with a differentiation media and the aggregates are incubated for a period of time during which the aggregates differentiate into placental organoids. The composition can be used to assess drug toxicity and drug screening.

Abstract: Early vascular cells (EVCs), including endothelial cells and pericytes, are generated from hiPSCs. Unlike the isolated endothelial progenitor cells, the differentiated ECs mature and are functional. When encapsulated in synthetic hydrogel, EVCs respond to matrix cues and self-assembled to form three-dimensional EVCs. Moreover, these EVCs respond to hypoxic microenvironment and undergo vasculogenesis to form complex 3D networks.

Abstract: Early vascular cells (EVCs), including endothelial cells and pericytes, are generated from hiPSCs. Unlike the isolated endothelial progenitor cells, the differentiated ECs mature and are functional. When encapsulated in synthetic hydrogel, EVCs respond to matrix cues and self-assembled to form three-dimensional EVCs. Moreover, these EVCs respond to hypoxic microenvironment and undergo vasculogenesis to form complex 3D networks.