Abstract: The invention relates to a method for producing a modified bacteriophage in a cell-free expression system wherein the expression of at least one gene of interest is suppressed by a molecule specifically inhibiting its expression. The invention further relates to a composition and a kit for producing a modified bacteriophage. Moreover, the invention relates to a bacteriophage which is not modified on the genomic level but on the proteomic level and its use for therapy, for diagnostic and detection assays.

Abstract: The present invention relates to a method for producing a bacteriophage in a cell-free host-independent expression system and a corresponding composition comprising a cell lysate of an organism which is different to the host of the bacteriophage, a at least one bacteriophage-host specific factor and a genome of a bacteriophage. The respective kits are also encompassed. The invention moreover refers to a bacteriophage obtained by the method of the invention and uses thereof.