Abstract: This application proposes a polyamide-imide copolymer film and a flexible display. The polyamide-imide copolymer film is obtained by copolymerizing aromatic dianhydride and aromatic dicarbonyl compound with aromatic diamine. Wherein, the aromatic dicarbonyl compounds include fluorinated aromatic dicarbonyl compound and non-fluorinated aromatic dicarbonyl compound. The polyamide-imide copolymer film can obtain a very low yellow degree index and dual refractive index, which is suitable for the substrate production of flexible display.

Abstract: The present disclosure relates, according to some embodiments, to immobilized enzyme compositions and methods for cleaving polynucleotide molecules including, for example, double-stranded DNA. Immobilized enzymes may comprise, for example, an enzyme (e.g., a type IIS restriction endonuclease, an RNAP, a capping enzyme), a support (e.g., a magnetic bead), and optionally, a linker disposed between the enzyme and the support. In some embodiments, methods may include contacting an immobilized enzyme with a polynucleotide substrate to form reaction products, separating the immobilized enzyme from the reaction products, and optionally reusing the immobilized enzymes in one or more subsequent reactions. preparing a library for sequencing.

Abstract: The present disclosure relates, according to some embodiments, to methods for preparing a library for sequencing. For example, a method may comprise (a) in a coupled reaction, (i) contacting a population of nucleic acid fragments with a tailing enzyme to produce tailed fragments, and (ii) ligating to the tailed fragments a sequencing adapter with a ligase to produce adapter-tagged fragments; and/or separating adapter-tagged fragments from the tailing enzyme and the ligase to produce separated adapter-tagged fragments and, optionally, separated tailing enzyme and/or separated ligase. In some embodiments, a tailing enzyme and/or a ligase used in library preparation may be immobilized enzymes.

Abstract: Provided herein is a thermolabile proteinase and methods of using the same. In some embodiments, the thermolabile proteinase may comprise an amino acid sequence that is at least 90% identical to any of SEQ ID NOs:1-11 and at least one amino acid substitution in helix 3. The thermolabile proteinase is active at a temperature in the range of 4° C.-40° C. and is inactivated by raising the temperature to above 50° C., where the proteinase is substantially inactive at 65° C.

Abstract: The present disclosure relates, according to some embodiments, to methods for preparing a library for sequencing. For example, a method may comprise (a) in a coupled reaction, (i) contacting a population of nucleic acid fragments with a tailing enzyme to produce tailed fragments, and (ii) ligating to the tailed fragments a sequencing adapter with a ligase to produce adapter-tagged fragments; and/or separating adapter-tagged fragments from the tailing enzyme and the ligase to produce separated adapter-tagged fragments and, optionally, separated tailing enzyme and/or separated ligase. In some embodiments, a tailing enzyme and/or a ligase used in library preparation may be immobilized enzymes.

Abstract: The present disclosure relates, according to some embodiments, to methods for preparing a library for sequencing. For example, a method may comprise (a) in a coupled reaction, (i) contacting a population of nucleic acid fragments with a tailing enzyme to produce tailed fragments, and (ii) ligating to the tailed fragments a sequencing adapter with a ligase to produce adapter-tagged fragments; and/or separating adapter-tagged fragments from the tailing enzyme and the ligase to produce separated adapter-tagged fragments and, optionally, separated tailing enzyme and/or separated ligase. In some embodiments, a tailing enzyme and/or a ligase used in library preparation may be immobilized enzymes.

Abstract: Provided herein is a thermolabile proteinase and methods of using the same. In some embodiments, the thermolabile proteinase may comprise an amino acid sequence that is at least 90% identical to any of SEQ ID NOs:1-11 and at least one amino acid substitution in helix 3. The thermolabile proteinase is active at a temperature in the range of 4° C.-40° C. and is inactivated by raising the temperature to above 50° C., where the proteinase is substantially inactive at 65° C.

Abstract: Provided herein is a thermolabile proteinase and methods of using the same. In some embodiments, the thermolabile proteinase may comprise an amino acid sequence that is at least 90% identical to any of SEQ ID NOs:1-11 and at least one amino acid substitution in helix 3. The thermolabile proteinase is active at a temperature in the range of 4° C.-40° C. and is inactivated by raising the temperature to above 50° C., where the proteinase is substantially inactive at 65° C.

Abstract: Provided herein is a thermolabile proteinase and methods of using the same. In some embodiments, the thermolabile proteinase may comprise an amino acid sequence that is at least 90% identical to any of SEQ ID NOs:1-11 and at least one amino acid substitution in helix 3. The thermolabile proteinase is active at a temperature in the range of 4° C.-40° C. and is inactivated by raising the temperature to above 50° C., where the proteinase is substantially inactive at 65° C.

Abstract: A computing device receives a first digital signal from a data acquisition system. It is determined that a first value includes in the first signal indicates an event. A second digital signal is received from the data acquisition system. It is determined that a second value included in the second signal includes a value that indicates the event, and that the event has occurred based at least in part on the first value, the second value, and a passage of a predetermined duration of time between a time of the first digital signal and a time of the second digital signal.

Abstract: Compositions and methods are provided for efficiently preparing a completely deglycosylated antibody where efficiency is measured in relative amounts of reagents in soluble or lyophilized form, and time and temperature of the reaction. Compositions and methods are also provided for separating substantially all N-linked glycans from a glycosylated antibody and for preserving functionality of the antibody. The methods are compatible with glycan labeling and protease digestion without the need for prior purification steps.

Abstract: Compositions and methods are provided for efficiently preparing a completely deglycosylated antibody where efficiency is measured in relative amounts of reagents in soluble or lyophilized form, and time and temperature of the reaction. Compositions and methods are also provided for separating substantially all N-linked glycans from a glycosylated antibody and for preserving functionality of the antibody. The methods are compatible with glycan labeling and protease digestion without the need for prior purification steps.

Abstract: Compositions and methods are provided for efficiently preparing a completely deglycosylated antibody where efficiency is measured in relative amounts of reagents in soluble or lyophilized form, and time and temperature of the reaction. Compositions and methods are also provided for separating substantially all N-linked glycans from a glycosylated antibody and for preserving functionality of the antibody. The methods are compatible with glycan labeling and protease digestion without the need for prior purification steps.

Abstract: A computing device receives a first digital signal from a data acquisition system. It is determined that a first value includes in the first signal indicates an event. A second digital signal is received from the data acquisition system. It is determined that a second value included in the second signal includes a value that indicates the event, and that the event has occurred based at least in part on the first value, the second value, and a passage of a predetermined duration of time between a time of the first digital signal and a time of the second digital signal.

Abstract: Anti-theft lockset is disclosed. Lock column and frame body are arranged in lock core. Outer side of lock column is matched with a long slot on the inner wall of lock shell. Inner side of lock column is provided with a crown pillar, and lock core is provided with a sliding chute for causing lock column to slide and a first through hole for causing frame body to move. Crown pillar is contacted with a first lock ball. Lock core is internally provided with lock ball holes matched with first and second lock balls. One end of the second lock ball is contacted with the frame body. The lock core is provided with a containing slot for causing the first lock ball and a locking plate to move up and down. The containing slot is communicated with a key hole. The locking plate is provided with a second through hole.

Abstract: Anti-theft lockset is disclosed. Lock column and frame body are arranged in lock core. Outer side of lock column is matched with a long slot on the inner wall of lock shell. Inner side of lock column is provided with a crown pillar, and lock core is provided with a sliding chute for causing lock column to slide and a first through hole for causing frame body to move. Crown pillar is contacted with a first lock ball. Lock core is internally provided with lock ball holes matched with first and second lock balls. One end of the second lock ball is contacted with the frame body. The lock core is provided with a containing slot for causing the first lock ball and a locking plate to move up and down. The containing slot is communicated with a key hole. The locking plate is provided with a second through hole.

Abstract: Global motion between frames of a motion-compensated inter-frame encoded video sequence is estimated directly from the motion vectors encoded within the frames. For any particular frame, the motion vectors are first decoded, and a finite number of sets of vectors are selected. An affine or other geometrical transform is then used to generate a motion estimation for each set, and then the least median squared error present in each motion estimation is calculated for each estimation. The motion estimation with the smallest least median squared error is then selected as being representative of the global motion in the image of the frame. A panoramic image generating method and system makes uses of the global motion estimations thus obtained.

Abstract: A method of segmenting a sequence of video images according to scene activity, the method comprising: defining a first series of nodes in a first multi-dimensional space, each node corresponding to an image of the sequence of video images; defining a transformation function that maps each of the first series of nodes to a corresponding node in a second multi-dimensional space having a lower dimensionality than the first multi-dimensional space; applying said transformation function to each of the first series of nodes to define a second series of respective nodes in the second multi-dimensional space; applying a data clustering algorithm to the second series of nodes to identify clusters of nodes within the second multi-dimensional space, the data clustering algorithm being constrained by a measure of feature distance between a pair of clusters of nodes and a measure of temporal distance between the pair of clusters of nodes; determining a representative image from each cluster of nodes and plotting each repr

Abstract: Video data defining a series of images is processed to define a first series of nodes in a first multidimensional space, each of the first series of nodes corresponding to an image of the series of images and its location in the first space defined in dependence on features of the respective image. A transformation function maps each of the nodes in the first multidimensional space onto a respective node in a second lower dimensionality multidimensional space while maintaining neighborhood relationships between nodes. A second series of nodes in the second multidimensional space is defined in accordance with the transformation function and a clustering analysis is performed in dependence on the nodes of the second multidimensional space.

Abstract: The invention relates to estimating the global motion between frames of a motion-compensated inter-frame encoded video sequence, directly from the motion vectors encoded within the frames. For any particular frame, a motion estimation is determined from motion vectors direct from the frame's anchor frame to the frame in question. This motion estimation is then checked against pre-determined criteria, and where the criteria are not met, re-estimation along a different route is performed, using the bi-directional motion vectors contained within B-frames. A panoramic image generating method and system which makes uses of the global motion estimations thus obtained is also described.