Abstract: A microfluidic device includes a single rigid support and a first unit having a first chamber of nonzero volume delimited by walls of the support. A filter separates the first chamber into a first space and a second space, a first channel made in the support and a second channel. The device also includes a second unit including a second chamber, a third channel. The device further includes a first fluidic transfer channel between the first chamber and the second chamber, made in the support and opening on the one hand into said second chamber and on the other hand at a first bypass node in the second channel. The device including first flow switching means arranged for selecting connection of the first chamber to the exterior only, via the second channel only or to the second chamber only, through the first transfer channel.

Abstract: A microfluidic system is intended for the analysis of a biological sample containing biological species. The system includes an optical detection device having a source configured to emit an optical signal and at least one sensor having a capture surface defining an optical signal reading zone. The system also includes a microfluidic device having a support in which an amplification chamber, in which an amplification reaction can be carried out, is made, and having an input channel opening into the amplification chamber. The amplification chamber includes at least one first zone located in the sensor reading zone and at least one protuberance forming a recess intended to receive a compound for internal control of the amplification reaction and arranged to be located outside the sensor reading zone or configured to be opaque to said optical signal.

Abstract: Method for determining the sensitivity of a bacterial strain of interest to a viral strain of bacteriophages, the method comprising: a) preparing a sample, this comprising bringing bacteria, belonging to the bacterial strain of interest, into contact with bacteriophages, each bacteriophage belonging to the same viral strain, the bacteria being either in a liquid medium, or in an agar medium; b) placing the sample between a light source and an image sensor, the light source emitting a light wave in an emission spectral band comprised between 500 nm and 600 nm; c) illuminating the sample using the light source and acquiring at least one image of the sample, with the image sensor, in the emission spectral band, no image-forming optic being placed between the sample and the image sensor; d) on the basis of the acquired images, determining a sensitivity of the bacterial strain of interest to the viral strain.

Abstract: A microfluidic system is intended for the analysis of a biological sample containing biological species. The system includes an optical detection device having a source configured to emit an optical signal and at least one sensor having a capture surface defining an optical signal reading zone. The system also includes a microfluidic device having a support in which an amplification chamber, in which an amplification reaction can be carried out, is made, and having an input channel opening into the amplification chamber. The amplification chamber includes at least one first zone located in the sensor reading zone and at least one protuberance forming a recess intended to receive a compound for internal control of the amplification reaction and arranged to be located outside the sensor reading zone or configured to be opaque to said optical signal.

Abstract: A microfluidic device includes a single rigid support and a first unit having a first chamber of nonzero volume delimited by walls of the support. A filter separates the first chamber into a first space and a second space, a first channel made in the support and a second channel. The device also includes a second unit including a second chamber, a third channel. The device further includes a first fluidic transfer channel between the first chamber and the second chamber, made in the support and opening on the one hand into said second chamber and on the other hand at a first bypass node in the second channel. The device including first flow switching means arranged for selecting connection of the first chamber to the exterior only, via the second channel only or to the second chamber only, through the first transfer channel.