Abstract: System and method of bioprinting used to enable automated fabrication of various constructs with high reproducibility and scalability, while reducing costs and production timelines. The bioprinting applications provides a critical component to the further enrichment the overall biomanufacturing paradigm. The biofabrication of sheet-like implantable constructs and other construct types with cells deposited on both sides—a process that may be both scaffold and cell type agnostic, and furthermore, is amenable to many additional tissue engineering applications beyond skeletal muscle.