Patents by Inventor Rachel Kasinskas
Rachel Kasinskas has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240067939Abstract: The present disclosure provides methods, compositions, kits and systems for nucleic acid amplification. In some embodiments, nucleic acid amplification methods include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, nucleic acid amplification methods include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the nucleic acid amplification method employs an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel and/or in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components.Type: ApplicationFiled: July 12, 2023Publication date: February 29, 2024Inventors: Chieh-Yuan LI, David RUFF, Shiaw-Min CHEN, Jennifer O'NEIL, Rachel KASINSKAS, Jonathan ROTHBERG, Bin LI, Kai Qin LAO
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Patent number: 11725195Abstract: The present disclosure provides methods, compositions, kits and systems for nucleic acid amplification. In some embodiments, nucleic acid amplification methods include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, nucleic acid amplification methods include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the nucleic acid amplification method employs an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel and/or in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components.Type: GrantFiled: April 27, 2021Date of Patent: August 15, 2023Assignee: Life Technologies CorporationInventors: Chieh-Yuan Li, David Ruff, Shiaw-Min Chen, Jennifer O'Neil, Rachel Kasinskas, Jonathan Rothberg, Bin Li, Kai Qin Lao
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Publication number: 20230175051Abstract: Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.Type: ApplicationFiled: February 1, 2023Publication date: June 8, 2023Inventors: Bin Li, Kai Qin LAO, Jennifer O'NEIL, Jennifer KUNKEL, Kellie HALEY, Rachel KASINSKAS, Zhaochun MA, Pius BRZOSKA
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Patent number: 11578360Abstract: Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.Type: GrantFiled: October 13, 2020Date of Patent: February 14, 2023Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: Bin Li, Kai Qin Lao, Jennifer O'Neil, Jennifer Kunkel, Kellie Haley, Rachel Kasinskas, Zhaochun Ma, Pius Brzoska
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Publication number: 20210261929Abstract: The present disclosure provides methods, compositions, kits and systems for nucleic acid amplification. In some embodiments, nucleic acid amplification methods include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, nucleic acid amplification methods include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the nucleic acid amplification method employs an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel and/or in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components.Type: ApplicationFiled: April 27, 2021Publication date: August 26, 2021Inventors: Chieh-Yuan LI, David RUFF, Shiaw-Min CHEN, Jennifer O'NEIL, Rachel KASINSKAS, Jonathan ROTHBERG, Bin LI, Kai Qin LAO
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Patent number: 11001815Abstract: The present disclosure provides methods, compositions, kits and systems for nucleic acid amplification. In some embodiments, nucleic acid amplification methods include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, nucleic acid amplification methods include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the nucleic acid amplification method employs an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel and/or in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components.Type: GrantFiled: June 14, 2019Date of Patent: May 11, 2021Assignee: Life Technologies CorporationInventors: Chieh-Yuan Li, David Ruff, Shiaw-Min Chen, Jennifer O'Neil, Rachel Kasinskas, Jonathan Rothberg, Bin Li, Kai Qin Lao
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Patent number: 10913976Abstract: Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.Type: GrantFiled: March 12, 2019Date of Patent: February 9, 2021Assignee: Life Technologies CorporationInventors: Bin Li, Kai Lao, Jennifer O'Neil, Jennifer Kunkel, Kellie Haley, Rachel Kasinskas, Zhaochun Ma, Pius Brzoska
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Publication number: 20210032691Abstract: Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.Type: ApplicationFiled: October 13, 2020Publication date: February 4, 2021Inventors: Bin LI, Kai Qin LAO, Jennifer O'NEIL, Jennifer KUNKEL, Kellie HALEY, Rachel KASINSKAS, Zhaochun MA, Pius BRZOSKA
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Patent number: 10858695Abstract: In some embodiments, provided are methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer.Type: GrantFiled: October 23, 2018Date of Patent: December 8, 2020Assignee: Life Technologies CorporationInventors: Chieh-Yuan Li, David Ruff, Jennifer O'Neil, Rachel Kasinskas, Shiaw-Min Chen, Jonathan M. Rothberg, Bin Li, Kai Qin Lao
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Publication number: 20190338258Abstract: The present disclosure provides methods, compositions, kits and systems for nucleic acid amplification. In some embodiments, nucleic acid amplification methods include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, nucleic acid amplification methods include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the nucleic acid amplification method employs an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel and/or in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components.Type: ApplicationFiled: June 14, 2019Publication date: November 7, 2019Inventors: Chieh-Yuan LI, David RUFF, Shiaw-Min CHEN, Jennifer O'NEIL, Rachel KASINSKAS, Jonathan ROTHBERG, Bin LI, Kai Qin LAO
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Publication number: 20190264272Abstract: Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.Type: ApplicationFiled: March 12, 2019Publication date: August 29, 2019Inventors: Bin LI, Kai Lao, Jennifer O'NEIL, Jennifer KUNKEL, Kellie HALEY, Rachel KASINSKAS, Zhaochun MA, Pius BRZOSKA
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Patent number: 10329544Abstract: In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming a reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components.Type: GrantFiled: April 6, 2016Date of Patent: June 25, 2019Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: Chieh-Yuan Li, David Ruff, Shiaw-Min Chen, Jennifer O'Neil, Rachel Kasinskas, Jonathan Rothberg, Bin Li, Kai Qin Lao, Wolfgang Hinz
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Publication number: 20190119738Abstract: In some embodiments, provided are methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer.Type: ApplicationFiled: October 23, 2018Publication date: April 25, 2019Inventors: Chieh-Yuan LI, David RUFF, Jennifer O'NEIL, Rachel KASINSKAS, Shiaw-Min CHEN, Jonathan M. ROTHBERG, Bin LI, Kai Qin LAO
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Patent number: 10233488Abstract: Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.Type: GrantFiled: September 26, 2016Date of Patent: March 19, 2019Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: Bin Li, Kai Qin Lao, Jennifer O'Neil, Jennifer Kunkel, Kellie Haley, Rachel Kasinskas, Zhaochun Ma, Pius Brzoska
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Patent number: 10113195Abstract: In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming a reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components.Type: GrantFiled: July 1, 2015Date of Patent: October 30, 2018Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: Chieh-Yuan Li, David Ruff, Jennifer O'Neil, Rachel Kasinskas, Shiaw-Min Chen, Jonathan Rothberg, Bin Li, Kai Qin Lao
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Patent number: 9862988Abstract: The disclosure relates generally to methods, kits and systems for extracting biomolecules from a water-in-oil emulsion, including breaking a water-in-oil emulsion comprising a plurality of aqueous droplets in a continuous hydrophobic fraction using a breaking solution to produce a resulting reaction mixture containing one or more biomolecules and manipulating the resulting reaction mixture to form at least two phases, where one of the phases includes an aqueous phase containing the one or more biomolecules.Type: GrantFiled: March 3, 2017Date of Patent: January 9, 2018Assignee: Life Technologies CorporationInventors: Jeffrey Sabina, Ilya Zlatkovsky, Rachel Kasinskas
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Publication number: 20170175171Abstract: The disclosure relates generally to methods, kits and systems for extracting biomolecules from a water-in-oil emulsion, including breaking a water-in-oil emulsion comprising a plurality of aqueous droplets in a continuous hydrophobic fraction using a breaking solution to produce a resulting reaction mixture containing one or more biomolecules and manipulating the resulting reaction mixture to form at least two phases, where one of the phases includes an aqueous phase containing the one or more biomolecules.Type: ApplicationFiled: March 3, 2017Publication date: June 22, 2017Inventors: Jeffrey SABINA, Ilya ZLATKOVSKY, Rachel KASINSKAS
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Patent number: 9617296Abstract: The disclosure relates generally to methods, systems, compositions and kits for breaking a water-in-oil emulsion including one or more biomolecules dispersed in an aqueous phase of the water-in-oil emulsion. In some embodiments, the disclosure relates to obtaining a first emulsion including a continuous hydrophobic fraction and a discontinuous aqueous fraction, the aqueous fraction having one or more biomolecules dispersed therein, breaking the first emulsion by contacting the first emulsion with a breaking solution including a second emulsion, where the second emulsion includes a discontinuous phase of organic extraction solvent dispersed in a continuous aqueous phase, and centrifuging to separate the phases of the resulting mixture.Type: GrantFiled: April 5, 2012Date of Patent: April 11, 2017Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: Jeffrey Sabina, Ilya Zlatkovsky, Rachel Kasinskas
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Publication number: 20170067098Abstract: Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.Type: ApplicationFiled: September 26, 2016Publication date: March 9, 2017Inventors: Bin LI, Kai Qin LAO, Jennifer O'NEIL, Jennifer KUNKEL, Kellie HALEY, Rachel KASINSKAS, Zhaochun MA, Pius BRZOSKA
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Patent number: 9476080Abstract: Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.Type: GrantFiled: April 21, 2015Date of Patent: October 25, 2016Assignee: Life Technologies CorporationInventors: Bin Li, Kai Qin Lao, Jennifer O'Neil, Jennifer Kunkel, Kellie Haley, Rachel Kasinskas, Zhaochun Ma, Pius Brzoska