Patents by Inventor Rachel Kasinskas

Rachel Kasinskas has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10913976
    Abstract: Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.
    Type: Grant
    Filed: March 12, 2019
    Date of Patent: February 9, 2021
    Assignee: Life Technologies Corporation
    Inventors: Bin Li, Kai Lao, Jennifer O'Neil, Jennifer Kunkel, Kellie Haley, Rachel Kasinskas, Zhaochun Ma, Pius Brzoska
  • Publication number: 20210032691
    Abstract: Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.
    Type: Application
    Filed: October 13, 2020
    Publication date: February 4, 2021
    Inventors: Bin LI, Kai Qin LAO, Jennifer O'NEIL, Jennifer KUNKEL, Kellie HALEY, Rachel KASINSKAS, Zhaochun MA, Pius BRZOSKA
  • Patent number: 10858695
    Abstract: In some embodiments, provided are methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer.
    Type: Grant
    Filed: October 23, 2018
    Date of Patent: December 8, 2020
    Assignee: Life Technologies Corporation
    Inventors: Chieh-Yuan Li, David Ruff, Jennifer O'Neil, Rachel Kasinskas, Shiaw-Min Chen, Jonathan M. Rothberg, Bin Li, Kai Qin Lao
  • Publication number: 20190338258
    Abstract: The present disclosure provides methods, compositions, kits and systems for nucleic acid amplification. In some embodiments, nucleic acid amplification methods include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, nucleic acid amplification methods include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the nucleic acid amplification method employs an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel and/or in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components.
    Type: Application
    Filed: June 14, 2019
    Publication date: November 7, 2019
    Inventors: Chieh-Yuan LI, David RUFF, Shiaw-Min CHEN, Jennifer O'NEIL, Rachel KASINSKAS, Jonathan ROTHBERG, Bin LI, Kai Qin LAO
  • Publication number: 20190264272
    Abstract: Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.
    Type: Application
    Filed: March 12, 2019
    Publication date: August 29, 2019
    Inventors: Bin LI, Kai Lao, Jennifer O'NEIL, Jennifer KUNKEL, Kellie HALEY, Rachel KASINSKAS, Zhaochun MA, Pius BRZOSKA
  • Patent number: 10329544
    Abstract: In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming a reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components.
    Type: Grant
    Filed: April 6, 2016
    Date of Patent: June 25, 2019
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Chieh-Yuan Li, David Ruff, Shiaw-Min Chen, Jennifer O'Neil, Rachel Kasinskas, Jonathan Rothberg, Bin Li, Kai Qin Lao, Wolfgang Hinz
  • Publication number: 20190119738
    Abstract: In some embodiments, provided are methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer.
    Type: Application
    Filed: October 23, 2018
    Publication date: April 25, 2019
    Inventors: Chieh-Yuan LI, David RUFF, Jennifer O'NEIL, Rachel KASINSKAS, Shiaw-Min CHEN, Jonathan M. ROTHBERG, Bin LI, Kai Qin LAO
  • Patent number: 10233488
    Abstract: Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.
    Type: Grant
    Filed: September 26, 2016
    Date of Patent: March 19, 2019
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Bin Li, Kai Qin Lao, Jennifer O'Neil, Jennifer Kunkel, Kellie Haley, Rachel Kasinskas, Zhaochun Ma, Pius Brzoska
  • Patent number: 10113195
    Abstract: In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming a reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components.
    Type: Grant
    Filed: July 1, 2015
    Date of Patent: October 30, 2018
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Chieh-Yuan Li, David Ruff, Jennifer O'Neil, Rachel Kasinskas, Shiaw-Min Chen, Jonathan Rothberg, Bin Li, Kai Qin Lao
  • Patent number: 9862988
    Abstract: The disclosure relates generally to methods, kits and systems for extracting biomolecules from a water-in-oil emulsion, including breaking a water-in-oil emulsion comprising a plurality of aqueous droplets in a continuous hydrophobic fraction using a breaking solution to produce a resulting reaction mixture containing one or more biomolecules and manipulating the resulting reaction mixture to form at least two phases, where one of the phases includes an aqueous phase containing the one or more biomolecules.
    Type: Grant
    Filed: March 3, 2017
    Date of Patent: January 9, 2018
    Assignee: Life Technologies Corporation
    Inventors: Jeffrey Sabina, Ilya Zlatkovsky, Rachel Kasinskas
  • Publication number: 20170175171
    Abstract: The disclosure relates generally to methods, kits and systems for extracting biomolecules from a water-in-oil emulsion, including breaking a water-in-oil emulsion comprising a plurality of aqueous droplets in a continuous hydrophobic fraction using a breaking solution to produce a resulting reaction mixture containing one or more biomolecules and manipulating the resulting reaction mixture to form at least two phases, where one of the phases includes an aqueous phase containing the one or more biomolecules.
    Type: Application
    Filed: March 3, 2017
    Publication date: June 22, 2017
    Inventors: Jeffrey SABINA, Ilya ZLATKOVSKY, Rachel KASINSKAS
  • Patent number: 9617296
    Abstract: The disclosure relates generally to methods, systems, compositions and kits for breaking a water-in-oil emulsion including one or more biomolecules dispersed in an aqueous phase of the water-in-oil emulsion. In some embodiments, the disclosure relates to obtaining a first emulsion including a continuous hydrophobic fraction and a discontinuous aqueous fraction, the aqueous fraction having one or more biomolecules dispersed therein, breaking the first emulsion by contacting the first emulsion with a breaking solution including a second emulsion, where the second emulsion includes a discontinuous phase of organic extraction solvent dispersed in a continuous aqueous phase, and centrifuging to separate the phases of the resulting mixture.
    Type: Grant
    Filed: April 5, 2012
    Date of Patent: April 11, 2017
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Jeffrey Sabina, Ilya Zlatkovsky, Rachel Kasinskas
  • Publication number: 20170067098
    Abstract: Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.
    Type: Application
    Filed: September 26, 2016
    Publication date: March 9, 2017
    Inventors: Bin LI, Kai Qin LAO, Jennifer O'NEIL, Jennifer KUNKEL, Kellie HALEY, Rachel KASINSKAS, Zhaochun MA, Pius BRZOSKA
  • Patent number: 9476080
    Abstract: Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.
    Type: Grant
    Filed: April 21, 2015
    Date of Patent: October 25, 2016
    Assignee: Life Technologies Corporation
    Inventors: Bin Li, Kai Qin Lao, Jennifer O'Neil, Jennifer Kunkel, Kellie Haley, Rachel Kasinskas, Zhaochun Ma, Pius Brzoska
  • Publication number: 20160272954
    Abstract: In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming a reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components.
    Type: Application
    Filed: April 6, 2016
    Publication date: September 22, 2016
    Inventors: Chieh-Yuan LI, David RUFF, Shiaw-Min CHEN, Jennifer O'NEIL, Rachel KASINSKAS, Jonathan ROTHBERG, Bin LI, Kai Qin LAO, Wolfgang HINZ
  • Patent number: 9371557
    Abstract: In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming a reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components.
    Type: Grant
    Filed: June 20, 2013
    Date of Patent: June 21, 2016
    Assignee: Life Technologies Corporation
    Inventors: Chieh-Yuan Li, David Ruff, Jennifer O'Neil, Rachel Kasinskas, Shiaw-Min Chen, Jonathan Rothberg, Bin Li, Kai Qin Lao
  • Patent number: 9334531
    Abstract: In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming a reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components.
    Type: Grant
    Filed: September 10, 2013
    Date of Patent: May 10, 2016
    Assignee: Life Technologies Corporation
    Inventors: Chieh-Yuan Li, David Ruff, Shiaw-Min Chen, Jennifer O'Neil, Rachel Kasinskas, Jonathan Rothberg, Bin Li, Kai Qin Lao
  • Patent number: 9309558
    Abstract: In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming a reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components.
    Type: Grant
    Filed: November 20, 2013
    Date of Patent: April 12, 2016
    Assignee: Life Technologies Corporation
    Inventors: Chieh-Yuan Li, David Ruff, Jennifer O'Neil, Rachel Kasinskas, Shiaw-Min Chen, Jonathan Rothberg, Bin Li, Kai Qin Lao
  • Patent number: 9309557
    Abstract: In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming a reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: April 12, 2016
    Assignee: Life Technologies Corporation
    Inventors: Chieh-Yuan Li, David Ruff, Shiaw-Min Chen, Jennifer O'Neil, Rachel Kasinskas, Jonathan Rothberg, Bin Li, Kai Qin Lao
  • Patent number: 9309566
    Abstract: In some embodiments, the present teachings provide methods for paired end sequencing. In some embodiment, a polynucleotide template to be subjected to paired end sequencing comprises at least one cross linking moiety and at least one scissile moiety. In some embodiments, a paired end sequencing reaction comprises (a) a forward sequencing step, (b) a cleavage step, and (c) a reverse sequencing step. In some embodiments, a paired end sequencing reaction comprises (a) a forward sequencing step, (b) a cross-linking step, (c) a cleavage step, and (d) a reverse sequencing step.
    Type: Grant
    Filed: March 14, 2013
    Date of Patent: April 12, 2016
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Bin Li, Kai Qin Lao, Jennifer O'Neil, Jennifer Kunkel, Kellie Haley, Rachel Kasinskas, Zhaochun Ma, Pius Brzoska