Patents by Inventor Radoslav GOLDMAN

Radoslav GOLDMAN has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20230213533
    Abstract: The instant disclosure provides methods of detecting N-glycopeptides in a sample by contacting the sample with one or more exoglycosidases and detecting the N-glycopeptides by mass spectrometry. Also provided are methods of detecting the presence or progression of a liver disease and treating said liver disease.
    Type: Application
    Filed: May 19, 2022
    Publication date: July 6, 2023
    Inventors: Radoslav GOLDMAN, Miloslav SANDA
  • Publication number: 20220252616
    Abstract: Provided is a sensitive and specific LC-MS-MRM quantification method that distinguishes outer-arm and core fucosylated configurations of N-glycopeptides. Advantage is taken of limited fragmentation of the glycopeptides at low collision energy (collision-induced dissociation) CID to produce linkage-specific Y-ions. These ions are selected as multiple reaction monitoring (MRM) transitions for the quantification of the outer-arm and total fucosylation of 23 glycoforms of 9 glycopeptides in 7 plasma proteins. The method permits quantification of the glycoforms directly in plasma or serum without fractionation of samples or glycopeptide enrichment. A pilot study of fucosylation in liver cirrhosis of hepatitis C vims (HCV) and non-alcoholic steatohepatitis (NASH) etiologies demonstrated that liver cirrhosis is consistently associated with increased outer-arm fucosylation of a majority of the analyzed proteins.
    Type: Application
    Filed: June 11, 2020
    Publication date: August 11, 2022
    Inventors: Radoslav GOLDMAN, Miloslav SANDA
  • Patent number: 11035865
    Abstract: Provided are in vitro serologic methods of assessing the presence of, and assessing the progression of, liver fibrosis in a subject. Also provided are methods of assessing efficacy of an agent for the treatment of liver fibrosis, and methods of treating liver fibrosis. The methods involve quantitatively measuring di-sialylated and mono-sialylated O-glycoforms of a peptide fragment of hemopexin (HPX) in a test serum sample obtained from a test subject, and comparing the measured amounts of di-sialylated and mono-sialylated O-glycoforms of the peptide fragment of hemopexin to a reference amount. In certain embodiments, the measuring is performed using LC-MS/MS-MRM (liquid chromatography/tandem mass spectrometry/multiple reaction monitoring). In certain embodiments, the measuring is performed using LC/MS3 (liquid chromatography with triple-stage mass spectrometric detection).
    Type: Grant
    Filed: August 31, 2017
    Date of Patent: June 15, 2021
    Assignee: GEORGETOWN UNIVERSITY
    Inventors: Radoslav Goldman, Miloslav Sanda
  • Publication number: 20190242907
    Abstract: Provided are in vitro serologic methods of assessing the presence of, and assessing the progression of, liver fibrosis in a subject. Also provided are methods of assessing efficacy of an agent for the treatment of liver fibrosis, and methods of treating liver fibrosis. The methods involve quantitatively measuring di-sialylated and mono-sialylated O-glycoforms of a peptide fragment of hemopexin (HPX) in a test serum sample obtained from a test subject, and comparing the measured amounts of di-sialylated and mono-sialylated O-glycoforms of the peptide fragment of hemopexin to a reference amount. In certain embodiments, the measuring is performed using LC-MS/MS-MRM (liquid chromatography/tandem mass spectrometry/multiple reaction monitoring). In certain embodiments, the measuring is performed using LC/MS3 (liquid chromatography with triple-stage mass spectrometric detection).
    Type: Application
    Filed: August 31, 2017
    Publication date: August 8, 2019
    Inventors: Radoslav GOLDMAN, Miloslav SANDA