Abstract: A system for storing a pharmaceutical composition at low temperatures includes: a pharmaceutical container having a barrel, a stopper which fluidly closes a first end of the barrel, and a closure which fluidly closes a second end of the barrel; and a liquid pharmaceutical composition and a gas both arranged within the barrel of the container between the stopper and the closure.

Abstract: A method of determining a tightness of a container includes: providing a container having an initial content at a first temperature, the container including a container body and at least one closure device applied to the container body; exposing the container to an ambient liquid during a first predetermined period of time, where during the first predetermined period of time the ambient liquid has a second temperature different from the first temperature; and after the first predetermined period of time has lapsed, determining a tightness of the container based on an amount of the ambient liquid and/or an amount of the initial content of the container having passed across a boundary of the container.