Patents by Inventor Rainer Christine

Rainer Christine has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Circuit arrangement for injecting nucleic acids and other biologically active molecules into the nucleus of higher eucaryotic cells using electrical current
    Patent number: 8173416
    Abstract: The invention relates to a novel circuit arrangement for electrotransfection or electrofusion, which enables the transportation of DNA and/or other biologically active molecules to the nucleus of higher eukaryotic cells or the fusion of cells, independent of cell division and with reduced cell mortality.
    Type: Grant
    Filed: April 23, 2002
    Date of Patent: May 8, 2012
    Assignee: Lonza Cologne GmbH
    Inventors: Herbert Müller-Hartmann, Gudula Riemen, Kirsten Rothmann-Cosic, Corinna Thiel, Ludger Altrogge, Meike Weigel, Rainer Christine, Elke Lorbach, Juliana Helfrich, Heike Wessendorf, Gregor Siebenkotten
  • Modular transfection systems
    Patent number: 7999073
    Abstract: The present invention relates to a method for transfection of cells using at least one protein capable of forming nucleoprotein filaments, wherein the protein is initially modified with at least one functional component which influences one or more steps of the transfection, the nucleic acid to be transfected is then loaded with the modified protein, whereby the nucleic acid and the protein form a filament-like complex, and this complex is finally added to the cells to be transfected. The invention further relates to a transfection agent consisting of nucleoprotein filaments (NPF), with at least one nucleoprotein filament-forming protein being modified with at least one functional component for the transfection. Furthermore, the present invention relates to the use of the transfection agent according to the invention for producing a drug for gene therapeutic treatment of humans and animals.
    Type: Grant
    Filed: November 21, 2007
    Date of Patent: August 16, 2011
    Assignee: Lonza Cologne GmbH
    Inventors: Hanns-Martin Schmidt, Ludger Altrogge, Dietmar Lenz, Gudula Riemen, Helmut Brosterhus, Elke Lorbach, Juliana Helfrich, Katharina Hein, Marion Gremse, Tatjana Males, Rainer Christine, Gregor Siebenkotten, Bodo Ortmann, Tamara Grabek, Andrea Mueller-Hartmann
  • CIRCUIT ARRANGEMENT FOR INJECTING NUCLEIC ACIDS AND OTHER BIOLOGICALLY ACTIVE MOLECULES INTO THE NUCLEUS OF HIGHER EUCARYONTIC CELLS USING ELECTRICAL CURRENT
    Publication number: 20090023131
    Abstract: The invention relates to a novel circuit arrangement for electrotransfection or electrofusion, which enables the transportation of DNA and/or other biologically active molecules to the nucleus of higher eukaryotic cells or the fusion of cells, independent of cell division and with reduced cell mortality.
    Type: Application
    Filed: August 9, 2007
    Publication date: January 22, 2009
    Applicant: AMAXA GMBH
    Inventors: Herbert Muller-Hartmann, Gudula Riemen, Kirsten Rothmann-Cosic, Corinna Thiel, Ludger Altrogge, Meike Weigel, Rainer Christine, Elke Lorbach, Juliana Helfrich, Heike Wessendorf, Gregor Siebenkotten
  • MODULAR TRANSFECTION SYSTEMS
    Publication number: 20080220527
    Abstract: The present invention relates to a method for transfection of cells using at least one protein capable of forming nucleoprotein filaments, wherein the protein is initially modified with at least one functional component which influences one or more steps of the transfection, the nucleic acid to be transfected is then loaded with the modified protein, whereby the nucleic acid and the protein form a filament-like complex, and this complex is finally added to the cells to be transfected. The invention further relates to a transfection agent consisting of nucleoprotein filaments (NPF), with at least one nucleoprotein filament-forming protein being modified with at least one functional component for the transfection. Furthermore, the present invention relates to the use of the transfection agent according to the invention for producing a drug for gene therapeutic treatment of humans and animals.
    Type: Application
    Filed: November 21, 2007
    Publication date: September 11, 2008
    Applicant: AMAXA AG
    Inventors: Hanns-Martin SCHMIDT, Ludger ALTROGGE, Dietmar LENZ, Gudula RIEMEN, Helmut BROSTERHUS, Elke LORBACH, Juliana HELFRICH, Katharina HEIN, Marion GREMSE, Tatjana MALES, Rainer CHRISTINE, Gregor SIEBENKOTTEN, Bodo ORTMANN, Tamara GRABECK, Andrea MULLER-HARTMANN
  • Modular transfection systems
    Patent number: 7320859
    Abstract: The present invention relates to a method for transfection of cells using at least one protein capable of forming nucleoprotein filaments, wherein the protein is initially modified with at least one functional component which influences one or more steps of the transfection, the nucleic acid to be transfected is then loaded with the modified protein, whereby the nucleic acid and the protein form a filament-like complex, and this complex is finally added to the cells to be transfected. The invention further relates to a transfection agent consisting of nucleoprotein filaments (NPF), with at least one nucleoprotein filament-forming protein being modified with at least one functional component for the transfection. Furthermore, the present invention relates to the use of the transfection agent according to the invention for producing a drug for gene therapeutic treatment of humans and animals.
    Type: Grant
    Filed: January 10, 2002
    Date of Patent: January 22, 2008
    Assignee: Amaxa AG
    Inventors: Hanns-Martin Schmidt, Ludger Altrogge, Dietmar Lenz, Gudula Riemen, Helmut Brosterhus, Elke Lorbach, Juliana Helfrich, Katharina Hein, Marion Gremse, Tatjana Males, Rainer Christine, Gregor Siebenkotten, Bodo Ortmann, Tamara Turbanski, Andreas Klaes
  • Circuit arrangement for injecting nucleic acids and other biologically active molecules into the nucleus of higher eucaryontic cells using electrical current
    Publication number: 20040137603
    Abstract: The invention relates to a novel circuit arrangement for electrotransfection or electrofusion, which enables the transportation of DNA and/or other biologically active molecules to the nucleus of higher eukaryotic cells or the fusion of cells, independent of cell division and with reduced cell mortality.
    Type: Application
    Filed: March 15, 2004
    Publication date: July 15, 2004
    Inventors: Herbert Muller-Hartmann, Gudula Riemen, Kirsten Rothmann-Cosic, Corinna Thiel, Ludger Altrogge, Meike Weigel, Rainer Christine, Elke Lorbach, Juliana Helfrich, Heike Wessendorf, Gregor Siebenkotten
  • Modular transfection systems
    Publication number: 20040137622
    Abstract: The present invention relates to a method for transfection of cells using at least one protein capable of forming nucleoprotein filaments, wherein the protein is initially modified with at least one functional component which influences one or more steps of the transfection, the nucleic acid to be transfected is then loaded with the modified protein, whereby the nucleic acid and the protein form a filament-like complex, and this complex is finally added to the cells to be transfected. The invention further relates to a transfection agent consisting of nucleoprotein filaments (NPF), with at least one nucleoprotein filament-forming protein being modified with at least one functional component for the transfection. Furthermore, the present invention relates to the use of the transfection agent according to the invention for producing a drug for gene therapeutic treatment of humans and animals.
    Type: Application
    Filed: August 13, 2003
    Publication date: July 15, 2004
    Inventors: Hanns-Martin Schmidt, Ludger Altrogge, Dietmar Lenz, Gudula Riemen, Helmut Brosterhus, Elke Lorbach, Juliana Helfrich, Katharina Hein, Marion Gremse, Tarjana Males, Rainer Christine, Gregor Siebenkotten, Bodo Ortmann, Andrea Klacs
  • Method for introducing nucleic acids and other biologically active molecules into the nucleus of higher eukaryotic cells by means of an electrical current
    Publication number: 20040014220
    Abstract: The invention relates to a novel method allowing the transport of DNA and/or other biologically active molecules into the nucleus of eukaryotic cells using electrical current, independently from cell division and with low cell mortality.
    Type: Application
    Filed: July 18, 2003
    Publication date: January 22, 2004
    Inventors: Gregor Siebenkotten, Rainer Christine, Ludger Altrogge, Marion Gremse, Dietmar Lenz, Sabine Poppenborg, Gudula Riemen, Kirsten Rothmann, Corinna Thiel
  • Cellular transport system for the transfer of a nucleic acid through the nuclear envelope and methods thereof
    Patent number: 6521456
    Abstract: The present invention relates to a nuclear transport agent, to a gene transfer system comprising said nuclear transport agent, to a method for transporting DNA into the nucleus of eukaryotic cells using said nuclear transport agent and to the use of said nuclear transport agent in gene therapy for treating cancer, viral infections, diseases of the nervous system, graft rejection and monogenic or polygenic hereditary diseases.
    Type: Grant
    Filed: July 6, 2001
    Date of Patent: February 18, 2003
    Inventors: Gregor Siebenkotten, Rainer Christine