Abstract: A process is described for obtaining insulin, which is virtually free from proteases and/or insulin acetylated at position A9, by chromatography in aqueous, buffered solvents which contain water-miscible organic solvents, on lipophilically modified silica gel, wherein the dissolving buffer contains acetone or acetonitrile.

Abstract: A device, consisting of a chromatography column which is closed off at the top by a plunger and, at the bottom, by a connection piece and has one or more lateral openings in the upper part, a container that contains suspended chromatography material and is connected via a line to one or more of the lateral openings, and a container that contains packing medium and is connected via a line to one or more of the lateral openings and/or is connected via a line to the plunger, is suitable for packing a chromatography column. A method for producing chromatography packings with the aid of the device is described.

Abstract: A process for the purification of insulins and insulin derivatives by chromatography in aqueous, buffered solvents which contain water-miscible organic solvents, on lipophilically modified silica gel, is described, wherein the aqueous, buffered solvents contain zwitterions, or the pH of the solvent mixture is in the vicinity of the isoelectric point of the insulin or insulin derivative to be purified and zwitterions are present.