Patents by Inventor Rainer Schmuck
Rainer Schmuck has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20170073661Abstract: Methods are provided for stabilizing an enzyme by storing the enzyme in the presence of a stabilized coenzyme. In addition, enzymes are provided that are stabilized with a stabilized coenzyme, as well as the use thereof in test elements for detecting analytes. Other aspects include unique compositions, methods, techniques, systems and devices involving enzyme stabilization.Type: ApplicationFiled: November 29, 2016Publication date: March 16, 2017Inventors: Wolfgang Roedel, Carina Horn, Nelli Steinke, Nadine Bucci, Thomas Meier, Rainer Schmuck, Rolf Nagel, Dieter Heindl
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Patent number: 9540702Abstract: Methods for stabilizing an enzyme by storing the enzyme in the presence of a stabilized coenzyme are disclosed. In addition, an enzyme stabilized with a stabilized coenzyme as well as the use thereof in test elements for detecting analytes are also disclosed. Other aspects include unique compositions, methods, techniques, systems and devices involving enzyme stabilization.Type: GrantFiled: February 20, 2012Date of Patent: January 10, 2017Assignee: Roche Diabetes Care, Inc.Inventors: Wolfgang Roedel, Carina Horn, Nelli Steinke, Nadine Bucci, Thomas Meier, Rainer Schmuck, Rolf Nagel, Dieter Heindl
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Patent number: 9359634Abstract: The present invention concerns a method for determining an analyte as well as a diagnostic element suitable therefore. In one particular form, a method for determining an analyte includes contacting a sample containing the analyte with a diagnostic element comprising a dry reagent layer. The dry reagent layer contains a mutated dehydrogenase which is specific for the analyte and an artificial coenzyme. The method also includes determining at least one of analyte presence and an amount of the analyte.Type: GrantFiled: August 16, 2011Date of Patent: June 7, 2016Assignee: Roche Diabetes Care, Inc.Inventors: Carina Horn, Claudia Gaessler-Dietsche, Dieter Heindl, Joachim Hoenes, Thomas Meier, Rainer Schmuck
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Publication number: 20140322737Abstract: The present invention concerns a method for determining an analyte as well as a diagnostic element suitable therefore. In one particular form, a method for determining an analyte includes contacting a sample containing the analyte with a diagnostic element comprising a dry reagent layer. The dry reagent layer contains a mutated dehydrogenase which is specific for the analyte and an artificial coenzyme. The method also includes determining at least one of analyte presence and an amount of the analyte.Type: ApplicationFiled: August 16, 2011Publication date: October 30, 2014Inventors: Carina Horn, Claudia Gaessler-Dietsche, Dieter Heindl, Joachim Hoenes, Thomas Meier, Rainer Schmuck
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Publication number: 20140142291Abstract: The disclosure concerns the enzymatic synthesis of stable analogues of nicotinamide adenine dinucleotide NAD/NADH and nicotinamide adenine dinucleotide phosphate NADP/NADPH, the so-called “carba-NADs”, i.e. analogues of NAD/NADH or NADP/NADPH, respectively, comprising a carbacyclic sugar instead of ribose.Type: ApplicationFiled: October 3, 2013Publication date: May 22, 2014Applicant: ROCHE DIAGNOSTICS OPERATIONS, INC.Inventors: Hartmut Duefel, Dieter Heindl, Carina Horn, Thomas Meier, Rainer Schmuck
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Patent number: 8580949Abstract: The disclosure concerns the enzymatic synthesis of stable analogues of nicotinamide adenine dinucleotide NAD/NADH and nicotinamide adenine dinucleotide phosphate NADP/NADPH, the so-called “carba-NADs”, i.e. analogues of NAD/NADH or NADP/NADPH, respectively, comprising a carbacyclic sugar instead of ribose.Type: GrantFiled: January 25, 2012Date of Patent: November 12, 2013Assignee: Roche Diagnostics Operations, Inc.Inventors: Hartmut Duefel, Dieter Heindl, Carina Horn, Thomas Meier, Rainer Schmuck
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Publication number: 20120276565Abstract: Methods for stabilizing an enzyme by storing the enzyme in the presence of a stabilized coenzyme are disclosed. In addition, an enzyme stabilized with a stabilized coenzyme as well as the use thereof in test elements for detecting analytes are also disclosed. Other aspects include unique compositions, methods, techniques, systems and devices involving enzyme stabilization.Type: ApplicationFiled: February 20, 2012Publication date: November 1, 2012Inventors: Wolfgang Roedel, Carina Horn, Nelli Steinke, Nadine Bucci, Thomas Meier, Rainer Schmuck, Rolf Nagel, Dieter Heindl
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Publication number: 20120130062Abstract: The disclosure concerns the enzymatic synthesis of stable analogues of nicotinamide adenine dinucleotide NAD/NADH and nicotinamide adenine dinucleotide phosphate NADP/NADPH, the so-called “carba-NADs”, i.e. analogues of NAD/NADH or NADP/NADPH, respectively, comprising a carbacyclic sugar instead of ribose.Type: ApplicationFiled: January 25, 2012Publication date: May 24, 2012Applicant: Roche Diagnostics Operations, Inc.Inventors: Hartmut Duefel, Dieter Heindl, Carina Horn, Thomas Meier, Rainer Schmuck
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Patent number: 8129106Abstract: A method for detecting sequence specific methylation in a biomolecule, comprising: (a) contacting the biomolecule with an S-adenosyl-L-methionine-dependent methyltransferase in the presence of a detectable cofactor of said methyltransferase; and (b) detecting whether the recognition sequence of said methyltransferase has been modified with the cofactor or a derivative thereof, wherein modification of the recognition sequence of said methyltransferase is indicative of an absence of methylation at said recognition sequence. Also disclosed is a cofactor specific for S-adenosyl-L-methionine-dependent methyltransferases, wherein said cofactor is an N-adenosylaziridine derivative with a reporter group attached to the 6 or 7 position of the adenine ring or attached to the aziridine ring. A complex of the cofactor and a methyltransferase a composition comprising the cofactor or the complex and the use of the cofactor or the complex for detecting sequence-specific methylation in DNA molecules are also disclosed.Type: GrantFiled: June 14, 2005Date of Patent: March 6, 2012Assignee: Max-Planck-Geselischaft zur Forderung der Wissenschaften E.V.Inventors: Elmar Weinhold, Thomas Meier, Hartmut Düfel, Christine Markert-Hahn, Rainer Schmuck
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Publication number: 20110165564Abstract: A method for detecting sequence specific methylation in a biomolecule, comprising: (a) contacting the biomolecule with an S-adenosyl-L-methionine-dependent methyltransferase in the presence of a detectable cofactor of said methyltransferase; and (b) detecting whether the recognition sequence of said methyltransferase has been modified with the cofactor or a derivative thereof, wherein modification of the recognition sequence of said methyltransferase is indicative of an absence of methylation at said recognition sequence. Also disclosed is a cofactor specific for S-adenosyl-L-methionine-dependent methyltransferases, wherein said cofactor is an N-adenosylaziridine derivative with a reporter group attached to the 6 or 7 position of the adenine ring or attached to the aziridine ring. A complex of the cofactor and a methyltransferase a composition comprising the cofactor or the complex and the use of the cofactor or the complex for detecting sequence-specific methylation in DNA molecules are also disclosed.Type: ApplicationFiled: June 14, 2005Publication date: July 7, 2011Applicant: MAX-PLANCK-GESELLSCHAFT ZUR FÖRDERUNG DER WISSENSCHAFTEN E.V.Inventors: Elmar Weinhold, Thomas Meier, Hartmut Düfel, Christine Markert-Hahn, Rainer Schmuck
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Patent number: 7972830Abstract: It was found that a fragment of native Thermus aquaticus DNA polymerase (TaqWT) lacking 288 N-terminal amino acids (Taq?288) possesses an increased thermostability over TaqWT, Taq?279, and Taq?289. The present invention therefore provides Taq?288, recombinant expression vectors encoding the same or derivatives thereof, as well as purification protocols for Taq?288. The invention also encompasses kits containing Taq?288 as well as the use of Taq?288 and kits containing Taq?288. In addition, the invention encompasses methods for the sequencing a nucleic acid template and methods for amplifying a target nucleic acid.Type: GrantFiled: August 12, 2004Date of Patent: July 5, 2011Assignee: Roche Diagnostics Operations, Inc.Inventors: Thomas Meier, Waltraud Ankenbauer, Annette Deufel, Dieter Heindl, Gisela Betzl, Rainer Schmuck, Bernd Schneidinger, Jessica Strey
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Patent number: 7781196Abstract: The present invention relates to a mutant protein of PQQ-dependent s-GDH characterized in that in at least one of the positions 122 and 124 the amino acid lysine is present, wherein these positions correspond to the amino acid positions known from the A. calcoaceticus s-GDH wild-type sequence (SEQ ID NO: 2), it also discloses genes encoding such mutant s-GDH, and different applications of these s-GDH mutants, particularly for determining the concentration of glucose in a sample.Type: GrantFiled: October 14, 2005Date of Patent: August 24, 2010Assignee: Roche Diagnostics Operations, Inc.Inventors: Mara Boenitz-Dulat, Peter Kratzsch, Rainer Schmuck
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Patent number: 7732179Abstract: A mutant of PQQ-dependent soluble glucose dehydrogenase (s-GDH; EC 1.1.5.2) is provided with improved specificity for glucose as compared to maltose, having a substitution of threonine at position 348 by either glycine, alamine or serine, wherein said mutant additionally comprises, at least one mutation for improving the stability of the mutant and one or more mutation(s) for improving the affinity of the mutant to glucose, and/or one or more mutation(s) for further improving the specificity of the mutant for glucose as compared to maltose, and wherein position 348 correspond to the amino acid positions known from the A. calcoaceticus s-GDH wild-type sequence. Also disclosed are genes encoding such mutant s-GDH, and different applications of these s-GDH mutants, particularly for determining the concentration of glucose in a sample.Type: GrantFiled: October 13, 2008Date of Patent: June 8, 2010Assignee: Roche Diagnostics Operations, Inc.Inventors: Mara Boenitz-Dulat, Daniela Beck, Peter Kratzsch, Rainer Schmuck, Herbert Von Der Eltz
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Publication number: 20090305316Abstract: The present invention relates to a mutant protein of PQQ-dependent s-GDH characterized in that in at least one of the positions 122 and 124 the amino acid lysine is present, wherein these positions correspond to the amino acid positions known from the A. calcoaceticus s-GDH wild-type sequence (SEQ ID NO: 2), it also discloses genes encoding such mutant s-GDH, and different applications of these s-GDH mutants, particularly for determining the concentration of glucose in a sample.Type: ApplicationFiled: October 14, 2005Publication date: December 10, 2009Inventors: Mara Boenitz-Dulat, Peter Kratzsch, Rainer Schmuck
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Patent number: 7547524Abstract: The present invention relates to improved variants of soluble pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenases (s-GDH) comprising an amino acid insertion between positions 428 and 429 as corresponding to the amino acid sequence known from Acinetobacter calcoaceticus, to genes encoding such variant s-GDH, to proteins of such s-GDH variants with improved substrate specificity for glucose, and to different applications of these s-GDH variants, particularly for determining concentrations of sugars, especially of glucose in a sample.Type: GrantFiled: July 19, 2005Date of Patent: June 16, 2009Assignee: Roche Diagnostics Operations, Inc.Inventors: Mara Boenitz-Dulat, Jessica Laggerbauer, Rainer Schmuck, Peter Kratzsch, Wolfgang-Reinhold Knappe
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Patent number: 7547535Abstract: The present invention relates to improved variants of soluble pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenases (s-GDH), to genes encoding mutated s-GDH, to mutant proteins of s-GDH with improved substrate specificity for glucose, and to different applications of these s-GDH variants, particularly for determining concentrations of sugar, especially of glucose in a sample.Type: GrantFiled: December 9, 2005Date of Patent: June 16, 2009Assignee: Roche Diagnostics Operations, Inc.Inventors: Peter Kratzsch, Rainer Schmuck, Daniela Beck, Zhixin Shao, Detlef Thym, Wolfgang-Reinhold Knappe
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Publication number: 20090148874Abstract: A mutant of PQQ-dependent soluble glucose dehydrogenase (s-GDH; EC 1.1.5.2) is provided with improved specificity for glucose as compared to maltose, having a substitution of threonine at position 348 by either glycine, alamine or serine, wherein said mutant additionally comprises, at least one mutation for improving the stability of the mutant and one or more mutation(s) for improving the affinity of the mutant to glucose, and/or one or more mutation(s) for further improving the specificity of the mutant for glucose as compared to maltose, and wherein position 348 correspond to the amino acid positions known from the A. calcoaceticus s-GDH wild-type sequence. Also disclosed are genes encoding such mutant s-GDH, and different applications of these s-GDH mutants, particularly for determining the concentration of glucose in a sample.Type: ApplicationFiled: October 13, 2008Publication date: June 11, 2009Inventors: Mara Boenitz-Dulat, Daniela Beck, Peter Kratzsch, Rainer Schmuck, Herbert Von Der Eltz
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Publication number: 20090143288Abstract: The current invention reports a polypeptide conjugate, wherein the conjugate comprises a first polypeptide selected from the group of polypeptides comprising SEQ ID NO: 01 and fragments thereof, and a second polypeptide selected from the group of antifusogenic peptides.Type: ApplicationFiled: March 10, 2008Publication date: June 4, 2009Inventors: Hartmut Duefel, Roberto Falkenstein, Iris Lein, Rainer Schmuck, Wilhelm Tischer
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Patent number: 7517664Abstract: The present invention relates to oxidoreductase apoenzyme variants which are enzymatically inactive but have coenzyme-binding properties. Further, the present invention relates to DNA sequences encoding these oxidoreductase apoenzyme variants, expression vectors containing such DNA sequences and the use of these oxidoreductase apoenzyme variants in diagnostic applications.Type: GrantFiled: November 14, 2006Date of Patent: April 14, 2009Assignee: Roche Diagnostics Operations, Inc.Inventors: Zhixin Shao, Joachim Hoenes, Carina Horn, Wolfgang-Reinhold Knappe, Rainer Schmuck
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Patent number: 7348423Abstract: A process is disclosed for the production of an antifusogenic peptide by producing a fusion peptide of a length of about 14 to 70 amino acids in a prokaryotic host cell, comprising the steps, under such conditions that inclusion bodies of said fusion peptide are formed, of: (a) expressing in said host cell a nucleic acid encoding said fusion peptide consisting of a first peptide which is an antifusogenic peptide of a length of about 10 to 50 amino acids and a second peptide of a length of about 4 to 30 amino acids, said first peptide being N-terminally linked to said second peptide; (b) cultivating said host cell to produce said inclusion bodies; and (c) recovering said antifusogenic peptide from said inclusion bodies, wherein said recovered antifusogenic peptide consists of said fusion peptide or a peptide comprising the antifusogenic peptide of about 10 to 50 amino acids and which is a fragment cleaved from said fusion peptide. Inclusion bodies of the peptides are disclosed.Type: GrantFiled: October 20, 2004Date of Patent: March 25, 2008Assignee: Hoffmann-La Roche Inc.Inventors: Eva Hoess, Thomas Meier, Gabriele Pestlin, Friedrich Popp, Klaus Reichert, Rainer Schmuck, Bernd Schneidinger, Christoph Seidel, Wilhelm Tischer