Abstract: A point-scanning luminescent microscope, especially for studying biological objects, has at least one collimated light source for producing an excitation light beam, an optical arrangement which focuses the light of the excitation light source on an object to be studied, at least one detector arrangement for acquiring light emitted by the object, an optical arrangement which collects the light emitted by the object and supplies it to the detector arrangement, and a scanner arrangement which causes relative movement between the scanning light beam and the object in at least two directions. The scanner arrangement has piezoactuators for achieving scanning movements between the scanning light beam and object. The detector arrangement can have a surface sensor which forms a confocal diaphragm. The light source can be designed to deliver rectangular pulses.

Abstract: An arrangement for confocal or quasifocal fluorescence microscopy is suggested in which, in an image beam path, there are arranged in succession, an objective arrangement for acquiring an image of a specimen to be examined, at least one scanner mirror located downstream of the objective arrangement, a tube lens, a confocal first strip diaphragm, a first spectrometer arrangement, a wavelength selection diaphragm for selection of the emission wavelength, a second spectrometer arrangement which is analogous to the first spectrometer arrangement, and a detector for acquiring the brightness distribution. In which, by means of the wavelength selection diaphragm, coupling of excitation light takes place by the reflecting back of an excitation beam path to the wavelength selection diaphragm from a source of monochromatic excitation light by a strip diaphragm which corresponds to the confocal strip diaphragm and a third spectrometer arrangement which is analogous to the first spectrometer arrangement.