Publication number: 20140342430
Abstract: A process for producing lipase by microorganisms on the industrial scale, wherein Escherichia coli microorganism harbouring lipase, preferably thermostable lipase, is fermented under batch or fed batch conditions, in a Luria Bertani (LB) broth supplemented with citric acid, (NH4)2SO4, Na2HPO4.12H2O, MgCl2, NH4Cl and glucose, using a trace metal solution containing hydrochloric acid, FeSO4.7H2O, ZnSO4.7H20, CaCl2.H20, MnSO4.5H20, CuSO4.5H2O, AlCl.6H2O, and H3BO3, and a feeding nutrient solution containing glucose, yeast extract, MgSO4.H2O, KH2PO4, Citric Acid, (NH4)2SO4, Na2HPO4.12H2O, MgCl2, and NH4Cl, at dissolved oxygen tension of 30-50%, at temperature of 30-40° C., while maintaining the pH between 6 and 8, with a glucose content of 15-25 g/L, with isopropylthiogalactoside.
Type:
Application
Filed:
May 19, 2014
Publication date:
November 20, 2014
Applicant:
Universiti Putra Malaysia (UPM)
Inventors:
Raja Noor Zaliha Raja Abdul RAHMAN, Abu Bakar SALLEH, Mahiran BASRI, Hafizal Mohd NIZAR