Patents by Inventor Rajagopal PANCHAPAKESAN
Rajagopal PANCHAPAKESAN has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240100532Abstract: A method includes flowing an incorporation reagent through a reagent management system and a flow cell of an instrument. The flow cell having a first polynucleotide positioned therein. The incorporation reagent adding a first base onto a sequence of bases. The sequence of bases includes a second polynucleotide complementary to the first polynucleotide. An image of an identification signal emanating from the first base is captured after the first base has been added onto the second polynucleotide. A cleavage reagent is flowed through the reagent management system and flow cell to remove a first terminator from the first base in order to enable a subsequent base in the sequence of bases to be added to the second polynucleotide. A buffer reagent is flowed through the reagent management system and flow cell in a plurality of cycles of consecutive forward and reverse flow directions.Type: ApplicationFiled: December 4, 2023Publication date: March 28, 2024Applicant: ILLUMINA, INC.Inventors: Tony YEN, Eric STAVA, Rajagopal PANCHAPAKESAN
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Patent number: 11911765Abstract: A method includes flowing an incorporation reagent through a reagent management system and a flow cell of an instrument. The flow cell having a first polynucleotide positioned therein. The incorporation reagent adding a first base onto a sequence of bases. The sequence of bases includes a second polynucleotide complementary to the first polynucleotide. An image of an identification signal emanating from the first base is captured after the first base has been added onto the second polynucleotide. A cleavage reagent is flowed through the reagent management system and flow cell to remove a first terminator from the first base in order to enable a subsequent base in the sequence of bases to be added to the second polynucleotide. A buffer reagent is flowed through the reagent management system and flow cell in a plurality of cycles of consecutive forward and reverse flow directions.Type: GrantFiled: June 16, 2020Date of Patent: February 27, 2024Assignee: ILLUMINA, INC.Inventors: Tony Yen, Eric Stava, Rajagopal Panchapakesan
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Publication number: 20230330671Abstract: A method includes engaging a well of a cartridge with a flow sensor of an instrument. The cartridge includes: a rotary valve including a rotatable port and a center port; the well in fluid communication with a channel, the channel including a channel port that the rotatable port is to align to in order to receive fluid from the well; and a flowcell including an inlet gasket in fluid communication with the center port. A source of pressurized air is connected to the flow sensor in order to establish a mass flow of air through a flow path. The flow path extends through one of the flow sensor, the channel, the rotary valve, and the flowcell. The mass flow of air through the flow path is measured with the flow sensor. It is determined if there is one of an air leak and an air blockage within the flow path.Type: ApplicationFiled: June 28, 2023Publication date: October 19, 2023Inventors: Sz-chin Steven Lin, Richard Lemoine, Rajagopal Panchapakesan, Wesley A. Cox-Muranami, Darren Segale
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Patent number: 11724258Abstract: A method includes engaging a well of a cartridge with a flow sensor of an instrument. The cartridge includes: a rotary valve including a rotatable port and a center port; the well in fluid communication with a channel, the channel including a channel port that the rotatable port is to align to in order to receive fluid from the well; and a flowcell including an inlet gasket in fluid communication with the center port. A source of pressurized air is connected to the flow sensor in order to establish a mass flow of air through a flow path. The flow path extends through one of the flow sensor, the channel, the rotary valve, and the flowcell. The mass flow of air through the flow path is measured with the flow sensor. It is determined if there is one of an air leak and an air blockage within the flow path.Type: GrantFiled: May 23, 2022Date of Patent: August 15, 2023Assignee: ILLUMINA, INC.Inventors: Sz-Chin Steven Lin, Richard Lemoine, Rajagopal Panchapakesan, Wesley A. Cox-Muranami, Darren Segale
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Publication number: 20230038505Abstract: Flow cell assemblies and related reagent selector valves. In accordance with an implementation, an apparatus includes a system including a reagent cartridge receptacle. The apparatus includes a flow cell assembly. The apparatus includes a reagent cartridge receivable within the reagent cartridge receptacle. The reagent cartridge including a plurality of reagent reservoirs. The apparatus includes a manifold assembly. The manifold assembly including a reagent selector valve adapted to be fluidically coupled to the reagent reservoirs and to selectively flow reagent from a corresponding reagent reservoir to the flow cell assembly. At least a surface of the manifold assembly associated with the reagent selector valve is coupled to a portion of the flow cell assembly.Type: ApplicationFiled: December 21, 2020Publication date: February 9, 2023Inventors: Bradley Drews, Reto Schoch, Tarun Khurana, Rajagopal Panchapakesan
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Publication number: 20220389500Abstract: A method for purifying nucleotides is provided, that includes preparing a solution comprising (a) 3?-blocked nucleotides, (b) 3?-OH nucleotides, (c) a polishing polymerase, and (d) a template. The polishing polymerase and the template are used to selectively polymerize the 3?-OH nucleotides and thus reduce a concentration in the solution of the 3?-OH nucleotides relative to the 3?-blocked nucleotides.Type: ApplicationFiled: May 19, 2022Publication date: December 8, 2022Applicants: ILLUMINA, INC., ILLUMINA CAMBRIDGE LIMITEDInventors: Kim Schneider, Jason Betley, Oliver Miller, Bradley Drews, Dominic Smith, Rajagopal Panchapakesan, Patrick McCauley, Stephen Mason, Tommaso Moschetti, Michael Chesney, Marta Richardson, Elliot Lawrence, Amanda Jackson, Rosamond Jackson, Erin Imsand, Henry Day
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Publication number: 20220339621Abstract: Valve assemblies and related systems are disclosed. An apparatus includes or comprises a system including or comprising an imaging system, a flow cell interface having a corresponding flow cell receptacle, a stage assembly, and a valve assembly including or comprising a valve and a valve drive assembly. The stage assembly moves the flow cell interface relative to the imaging system and the valve drive assembly is to drive the valve using shaped input signals to reduce vibration imposed on at least one of the stage assembly or another component of the apparatus based on movement of the valve.Type: ApplicationFiled: November 15, 2021Publication date: October 27, 2022Inventors: Ahmadreza Rashedi, Gregory Holst, Rajagopal Panchapakesan
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Publication number: 20220280940Abstract: A method includes engaging a well of a cartridge with a flow sensor of an instrument. The cartridge includes: a rotary valve including a rotatable port and a center port; the well in fluid communication with a channel, the channel including a channel port that the rotatable port is to align to in order to receive fluid from the well; and a flowcell including an inlet gasket in fluid communication with the center port. A source of pressurized air is connected to the flow sensor in order to establish a mass flow of air through a flow path. The flow path extends through one of the flow sensor, the channel, the rotary valve, and the flowcell. The mass flow of air through the flow path is measured with the flow sensor. It is determined if there is one of an air leak and an air blockage within the flow path.Type: ApplicationFiled: May 23, 2022Publication date: September 8, 2022Inventors: Sz-Chin Steven Lin, Richard Lemoine, Rajagopal Panchapakesan, Wesley A. Cox-Muranami, Darren Segale
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Patent number: 11351541Abstract: A method includes engaging a well of a cartridge with a flow sensor of an instrument. The cartridge includes: a rotary valve including a rotatable port and a center port; the well in fluid communication with a channel, the channel including a channel port that the rotatable port is to align to in order to receive fluid from the well; and a flowcell including an inlet gasket in fluid communication with the center port. A source of pressurized air is connected to the flow sensor in order to establish a mass flow of air through a flow path. The flow path extends through one of the flow sensor, the channel, the rotary valve, and the flowcell. The mass flow of air through the flow path is measured with the flow sensor. It is determined if there is one of an air leak and an air blockage within the flow path.Type: GrantFiled: April 2, 2018Date of Patent: June 7, 2022Assignee: Illumina, Inc.Inventors: Sz-Chin Steven Lin, Richard Lemoine, Rajagopal Panchapakesan, Wesley A. Cox-Muranami, Darren Segale
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Publication number: 20210379578Abstract: An apparatus and examples of methods for using and manufacturing aspects of an apparatus with a sensor having an active surface. A sensor, a lid, and a flow channel bounded by the lid and a surface of the sensor, and including an illumination source, a heater, and a pump. A method includes fluidically coupling a first flow cell and a second flow cell to a reservoir, moving fluid from the reservoir into a flow channel of the first and second flow cell using respective pumps; and heating fluid in the flow channels of the first and second flow cells using respective heaters. A method includes forming a first sensor and a second sensor on a flexible surface, and folding the flexible surface until the first sensor faces the second sensor.Type: ApplicationFiled: May 19, 2021Publication date: December 9, 2021Inventors: Wesley Cox-Muranami, Rajagopal Panchapakesan, Arnaud Rival
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Publication number: 20210299661Abstract: A method includes engaging a well of a cartridge with a flow sensor of an instrument. The cartridge includes: a rotary valve including a rotatable port and a center port; the well in fluid communication with a channel, the channel including a channel port that the rotatable port is to align to in order to receive fluid from the well; and a flowcell including an inlet gasket in fluid communication with the center port. A source of pressurized air is connected to the flow sensor in order to establish a mass flow of air through a flow path. The flow path extends through one of the flow sensor, the channel, the rotary valve, and the flowcell. The mass flow of air through the flow path is measured with the flow sensor. It is determined if there is one of an air leak and an air blockage within the flow path.Type: ApplicationFiled: April 2, 2018Publication date: September 30, 2021Inventors: Sz-Chin Steven LIN, Richard LEMOINE, Rajagopal PANCHAPAKESAN, Wesley A. COX-MURANAMI, Darren SEGALE
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Publication number: 20200398278Abstract: A method includes flowing an incorporation reagent through a reagent management system and a flow cell of an instrument. The flow cell having a first polynucleotide positioned therein. The incorporation reagent adding a first base onto a sequence of bases. The sequence of bases includes a second polynucleotide complementary to the first polynucleotide. An image of an identification signal emanating from the first base is captured after the first base has been added onto the second polynucleotide. A cleavage reagent is flowed through the reagent management system and flow cell to remove a first terminator from the first base in order to enable a subsequent base in the sequence of bases to be added to the second polynucleotide. A buffer reagent is flowed through the reagent management system and flow cell in a plurality of cycles of consecutive forward and reverse flow directions.Type: ApplicationFiled: June 16, 2020Publication date: December 24, 2020Inventors: Tony YEN, Eric STAVA, Rajagopal PANCHAPAKESAN
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Publication number: 20200108386Abstract: A method for testing a microfluidic device includes interfacing a microfluidic device to a fluidic parameter testing system. The microfluidic device has an internal rotary valve and internal fluidic channels. Each channel has a port with a predetermined port position that the rotary valve is to align to in order to select any one of a plurality of reagents which flow through the channels. The rotary valve is rotated via the testing system to a plurality of rotary valve position of the rotary valve. A fluidic parameter of the microfluidic device is measured at each rotary valve position. The fluidic parameter is mapped relative to the rotary valve positions. It is determined from the mapping if the rotary valve aligns with each of the predetermined port positions for a flow of the reagents through the channels.Type: ApplicationFiled: April 2, 2018Publication date: April 9, 2020Inventors: Wesley A. COX-MURANAMI, Sz-Chin Steven LIN, Rajagopal PANCHAPAKESAN, Darren SEGALE, Richard LEMOINE