Abstract: The present invention provides a novel beta glucosidase and a process for extraction of a beta-glucosidase from Rauvolfia serpentine useful for the cleaving of beta-1,4 linkage of PNPG and to convert other gluco-conjugates such as strictosidine and raucaffricine into their corresponding aglycon such as vomilenine, commercially through immobilizing the enzyme. The ? glucosidase enzyme has shown maximum activity in the acid pH range, with high optimum temperature using PNPG as substrate. The crude enzyme, when stored at 4° C., was quite stable for 6 days with 50% loss of activity. The enzyme was activated in presence of FeSO4 in the assay mixture.

Abstract: The present invention relates to an improved process of analytical and quantitative isolation of withaferin-A from Withania somnifera (Sanskrit: Ashwagandha, English: winter cherry) and other plants and products therefrom, said method comprising steps of selecting most appropriate extraction medium composition, with extraction solvent system consisting of a defined admixture of water and alcohol in a range of proportion of alcohol within a narrow range of (methanol, ethanol etc.

Abstract: The present invention relates to a method for profiling of protein extract from hyper-acidic aerial parts of plant Scented Geranium (Pelargonium sp.), said method comprising steps of selecting effective extraction medium, with extraction medium consisting of 0.2 M sodium carbonate increasing percentage extract by about 600%, selecting effective medium to determine polypeptide pattern in said extract, with polypeptide pattern medium consisting of Sodium Carbonate ranging between 0.2 to 0.4 M, and 0.2 M Sodium Carbonate along with 50% dimethylsulfoxide, selecting effective media to determine enzyme multimolecular forms in said extract, with enzyme multimolecular forms media consisting of 0.2 M Tris-HCl buffer at pH 7.5, and optionally 50% dimethylsulfoxide; Sodium Carbonate at concentration ranging between 0.2 to 0.

Abstract: The present invention relates to a method for profiling of protein extract from hyper-acidic aerial parts of plant Scented Geranium (Pelargonium sp.), said method comprising steps of selecting effective extraction medium, with extraction medium consisting of 0.2 M sodium carbonate increasing percentage extract by about 600%, selecting effective medium to determine polypeptide pattern in said extract, with polypeptide pattern medium consisting of Sodium Carbonate ranging between 0.2 to 0.4M, and 0.2 M Sodium Carbonate along with 50% dimethylsulfoxide, selecting effective media to determine enzyme multimolecular forms in said extract, with enzyme multimolecular forms media consisting of 0.2M Tris-HCl buffer at pH 7.5, and optionally 50% dimethylsulfoxide; Sodium Carbonate at concentration ranging between 0.2 to 0.

Abstract: The invention provides a process for the induction of normal rooting on nodal and internodal stem segments without using hormones and/or other chemical treatments in Mentha species, which comprises oblique excision of shoots of Mentha species from the plants, immediately dipping the cut end of twigs or stems into water and keeping in complete dark for up to one week with by varying the temperature cycle for each set of 24 hours duration of the treatment.

Abstract: The invention provides a process for the induction of normal rooting on nodal and internodal stem segments without using hormones and/or other chemical treatments in Mentha species, which comprises oblique excision of shoots of Mentha species from the plants, immediately dipping the cut end of twigs or stems into water and keeping in complete dark for up to one week with by varying the temperature cycle for each set of 24 hours duration of the treatment.

Abstract: The present invention provides a novel ecological method of phyto-remediation of alkaline ad chemically degraded soils, said method comprising the steps of dispersing biomass obtained from Pelargonium sp. in alkaline or chemically degraded soil.

Abstract: The present invention relates to an anthocyanin producing callus line in cultures of Panax sikkimensis (an Indian species of ginseng) said line comprising: (a) Characteristic pink-purple pigmentation, (b) Growth index of about 221-450 in about 50-80 days of culture period, (c) Yield of anthocyanin (2-3 mg/gf.wt.

Abstract: The invention provides stable high ginsenoside-yielding callus lines of Panax quinquefolium (American Ginseng). The callus lines are useful in the industrial production of ginsenosides for use in a variety of ginseng preparations.