Abstract: The present invention relates to a cleaning composition for cleaning medical instruments. The cleaning composition of the invention comprises: an enzyme activity protector complex; an enzyme system; one or more controlling polymers; surfactants; organic solvent; and additives selected from chelating agents, wetting agents, preservatives and water. In particular, the cleaning composition of the invention is a low foaming, multi enzymatic cleaner having excellent efficacy for enzymatic cleaning, hard water scale inhibition and rust inhibition due to scaling. The composition further provides shine with superior wetting and cleaning performance. The invention also relates to a process for preparing a cleaning composition and a method of cleaning medical instruments employing the cleaning composition of the present invention.