Abstract: A method comprising obtaining a substantially cell-free sample of blood plasma or blood serum from a subject with osteoarthritis; and detecting a presence of or measuring a level of novel_miRNA_1 (gucuggcucaggguuggg) (SEQ ID NO: 1), novel_miRNA_2 (ucccuguucgggcgccacu) (SEQ ID NO: 2), novel_miRNA_3 (uguuuagcauccuguagccugc) (SEQ ID NO: 3), and novel_miRNA_4 (uaguggguuaucagaacu) (SEQ ID NO: 4). Also provided are methods where additional miRNAs are detected including novel miRNA 5 (SEQ ID NO: 5), novel miRNA 6 (SEQ ID NO: 6), novel miRNA 7 (SEQ ID NO: 7), novel miRNA 8 (SEQ ID NO: 8), novel miRNA 9 (SEQ ID NO: 9), novel miRNA 10 (SEQ ID NO: 10), novel miRNA 11 (SEQ ID NO: 11), novel miRNA 12 (SEQ ID NO: 12), novel miRNA 13 (SEQ ID NO: 13), hsa-miR-335-3p, hsa-miR-199a-5p, hsa-miR-671-3p, hsa-miR-1260b, hsa-miR-191-3p, hsa-miR-335-5p and/or hsa-miR-543.

Abstract: A method staging of knee osteoarthritis (OA) comprising a) obtaining a substantially cell-free sample of blood plasma or blood serum from a subject with osteoarthritis; b) detecting a presence of or measuring a level of one or more miRNAs selected from hsa-miR-335-3p, hsa-miR-199a-5p, hsa-miR-671-3p, hsa-miR-1260b, hsa-miR-191-3p, hsa-miR-335-5p, hsa-miR-543, novel_miRNA_1 (gucuggcucaggguuggg) (SEQ ID NO: 1), novel_miRNA_2 (ucccuguucgggcgccacu) (SEQ ID NO: 2), novel_miRNA_3 (uguuuagcauccuguagccugc) (SEQ ID NO: 3), and novel_miRNA_4 (uaguggguuaucagaacu) (SEQ ID NO: 4); and c) identifying the subject as likely to have early stage osteoarthritis or late stage osteoarthritis based on the presence of or measured level of the one or more miRNAs. Isolated nucleic acids, primers, probes, panels and kits are also provided.

Abstract: A system and methodology that can optimize practical performance by reducing broadcast latency and collisions in a multihop wireless network is provided. The system includes an optimization component that determines a schedule for transmitting and/or receiving messages at nodes in the network during one-to-all broadcasting, all-to-all broadcasting, and/or all-to-one collection. The schedule enables each node in wireless network to participate in broadcasting as soon as possible, such that, when a node transmits a message to its parent to deliver the message to a source node, the children of the node also receive the message and initiate broadcasting the message in their subtrees in parallel. Further, during data collection, the schedule for messages to be transmitted to a sink node is determined based in part on a priority associated with the message. Additionally, the generated schedule does not affect a set of theoretical bounds associated with a broadcast algorithm employed by the network.

Abstract: A system and methodology that can optimize practical performance by reducing broadcast latency and collisions in a multihop wireless network is provided. The system includes an optimization component that determines a schedule for transmitting and/or receiving messages at nodes in the network during one-to-all broadcasting, all-to-all broadcasting, and/or all-to-one collection. The schedule enables each node in wireless network to participate in broadcasting as soon as possible, such that, when a node transmits a message to its parent to deliver the message to a source node, the children of the node also receive the message and initiate broadcasting the message in their subtrees in parallel. Further, during data collection, the schedule for messages to be transmitted to a sink node is determined based in part on a priority associated with the message. Additionally, the generated schedule does not affect a set of theoretical bounds associated with a broadcast algorithm employed by the network.

Abstract: A method of advertising using a user profile based on a user's interaction with websites, including the time spent on the website and the user's rating of keywords associated with the contents of the website.