Patents by Inventor Rakesh Koppram

Rakesh Koppram has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10947571
    Abstract: The present invention provides a method for producing lactic acid in a recombinant yeast cell culture using glucose as carbon source comprising a first, seed fermentation stage to produce biomass wherein the yeast is cultivated in a culture medium at a pH of 5 to 7, followed by a second, a production fermentation stage with biomass from the seed fermentation to produce lactic acid, wherein the yeast is cultivated in a culture medium at low p H using a yeast strain that is engineered to have lactate dehydrogenase (LDH) activity and optionally has decreased or knocked-out pyruvate decarboxylase (PDC) activity.
    Type: Grant
    Filed: April 14, 2017
    Date of Patent: March 16, 2021
    Assignee: SYCONIUM LACTIC ACID GMBH
    Inventors: Magnus Ask, Rakesh Koppram, Diethard Mattanovich, Michael Sauer
  • Publication number: 20190127766
    Abstract: The present invention provides a method for producing lactic acid in a recombinant yeast cell culture using glucose as carbon source comprising a first, seed fermentation stage to produce biomass wherein the yeast is cultivated in a culture medium at a pH of 5 to 7, followed by a second, a production fermentation stage with biomass from the seed fermentation to produce lactic acid, wherein the yeast is cultivated in a culture medium at low p H using a yeast strain that is engineered to have lactate dehydrogenase (LDH) activity and optionally has decreased or knocked-out pyruvate decarboxylase (PDC) activity.
    Type: Application
    Filed: April 14, 2017
    Publication date: May 2, 2019
    Inventors: Magnus Ask, Rakesh Koppram, Diethard Mattanovich, Michael Sauer
  • Patent number: 9115376
    Abstract: A method for producing a strain of Saccharomyces cerevisiae with introduced genes coding for xylose reductase, xylitol dehydrogenase and xylulokinase and with improved ethanol production, improved xylose conversion and reduced xylitol production is described. The method comprises culturing the cells in a repetitive batch series in a medium at a xylose concentration of about 15-25 g/l and at a temperature of about 28-32° C., and thereafter lowering the xylose concentration in at least one step at a temperature of about 28-32° C. to obtain an increased selection pressure for improved xylose fermentation, improved ethanol production and reduced xylitol production, and continuing the culturing of the cells in said repetitive batch series. Further, strains of Saccharomyces cerevisiae obtained by the method according to the invention are described.
    Type: Grant
    Filed: November 15, 2011
    Date of Patent: August 25, 2015
    Assignee: SCANDINAVIAN TECHNOLOGY GROUP AB
    Inventors: Eva Albers, Lisbeth Olsson, Rakesh Koppram
  • Patent number: 8962289
    Abstract: A method for producing a strain of Saccharomyces cerevisiae with introduced genes coding for xylose reductase, xylitol dehydrogenase and xylulokinase and with improved ethanol production, improved xylose conversion, reduced xylitol production and improved inhibitor tolerance is described. The method comprises culturing a strain of Saccharomyces cerevisiae at a continuous mode with a medium comprising essentially only xylose as carbon source at a temperature of 25-38° C., preferably 30-35° C., and an airflow of 0.040-0.055 vvm, and increasing the dilution rate to maintain a constant cell level, said cell level being in the range of 1.5-3.0 determined by optical density or equivalent analytical means, and adding at least one inhibitor to the cells and gradually increasing the addition of said inhibitor. Further, strains of Saccharomyces cerevisiae obtained by the method according to the invention are described.
    Type: Grant
    Filed: November 15, 2011
    Date of Patent: February 24, 2015
    Assignee: Scandinavian Technology Group AB
    Inventors: Eva Albers, Lisbeth Olsson, Rakesh Koppram
  • Publication number: 20130295620
    Abstract: A method for producing a strain of Saccharomyces cerevisiae with introduced genes coding for xylose reductase, xylitol dehydrogenase and xylulokinase and with improved ethanol production, improved xylose conversion and reduced xylitol production is described. The method comprises culturing the cells in a repetitive batch series in a medium at a xylose concentration of about 15-25 g/l and at a temperature of about 28-32° C., and thereafter lowering the xylose concentration in at least one step at a temperature of about 28-32° C. to obtain an increased selection pressure for improved xylose fermentation,improved ethanol production and reduced xylitol production, and continuing the culturing of the cells in said repetitive batch series. Further, strains of Saccharomyces cerevisiae obtained by the method according to the invention are described.
    Type: Application
    Filed: November 15, 2011
    Publication date: November 7, 2013
    Applicant: SCANDINAVIAN TECHNOLOGY GROUP AB
    Inventors: Eva Albers, Lisbeth Olsson, Rakesh Koppram
  • Publication number: 20130244247
    Abstract: A method for producing a strain of Saccharomyces cerevisiae with introduced genes coding for xylose reductase, xylitol dehydrogenase and xylulokinase and with improved ethanol production, improved xylose conversion, reduced xylitol production and improved inhibitor tolerance is described. The method comprises culturing a strain of Saccharomyces cerevisiae at a continuous mode with a medium comprising essentially only xylose as carbon source at a temperature of 25-38° C., preferably 30-35° C., and an airflow of 0.040-0.055 vvm, and increasing the dilution rate to maintain a constant cell level, said cell level being in the range of 1.5-3.0 determined by optical density or equivalent analytical means, and adding at least one inhibitor to the cells and gradually increasing the addition of said inhibitor. Further, strains of Saccharomyces cerevisiae obtained by the method according to the invention are described.
    Type: Application
    Filed: November 15, 2011
    Publication date: September 19, 2013
    Applicant: SCANDINAVIAN TECHNOLOGY GROUP AB
    Inventors: Eva Albers, Lisbeth Olsson, Rakesh Koppram