Patents by Inventor Ralf Kuhn
Ralf Kuhn has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240132293Abstract: A feeding screw machine for feeding a processing screw machine comprises a housing with at least two housing bores formed therein and screw shafts rotatably arranged in the at least two housing bores. The at least two housing bores and the at least two screw shafts define a free volume in a respective cross-sectional plane, which decreases monotonically at least in some regions for continuous compression of a material in a conveying direction. This enables an improved supply of material with a low bulk density.Type: ApplicationFiled: January 28, 2022Publication date: April 25, 2024Applicant: Coperion GmbHInventors: Stefan HUBER, Markus SCHMUDDE, Svetlana MARINOVA, Ralf KÜHN, Kai KLUGE, Oleg RUDI, Dirk KYRION, Robert KESSLER, Leonid LIBER, Marina MATTA, Jochen SCHOFER
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Patent number: 11891635Abstract: A method for modifying double stranded DNA (dsDNA) employing an RNA guided DNA endonuclease to generate two double strand breaks in the dsDNA molecule to be modified, and replacement of the sequence positioned between the double strand breaks with a substitute DNA sequence using the non-homologous end joining (NHEJ) pathway, and corresponding kits and compositions for modifying double stranded DNA molecules.Type: GrantFiled: December 21, 2018Date of Patent: February 6, 2024Assignee: Max-Delbrück-Centrum für Molekulare Medizin in der Helmholtz-GemeinschaftInventors: Ralf Kühn, Eric Danner
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Publication number: 20230272861Abstract: The present invention pertains to a split ring assembly for adjusting the throughput of a fluid of a hydraulic valve comprising an elastomeric element being adapted to mount a split ring around a hydraulic ram, wherein the elastomeric element is arranged around the outward circumference of the split ring, wherein the throughput of the fluid is adjustable depending on the degree to which the throughput of hydraulic fluid is limited by the split ring.Type: ApplicationFiled: August 4, 2021Publication date: August 31, 2023Applicant: Caterpillar Inc.Inventors: Ralf KUHN, Sebastian LAMMERS
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Publication number: 20230264407Abstract: A filter unit for an extruder assembly comprises a support body with a hollow cylindrical wall section and a base section connected thereto, wherein the support body has a central longitudinal axis and delimits an inner space, wherein the hollow cylindrical wall section has a plurality of through openings opening into the inner space and forms an outlet opening for a molten plastic opposite the base section; and a displacement element for displacing the molten plastic which has entered the inner space, in particular via the through openings, substantially in the direction of the central longitudinal axis, wherein the displacement element and the support body are configured and/or produced in one piece, as well as filter arrangement with a plurality of such filter units, filter changing device with at least two filter arrangements and method for producing such a filter unit.Type: ApplicationFiled: February 22, 2023Publication date: August 24, 2023Applicant: Coperion GmbHInventors: Stefan HUBER, Tomi TRATNIK, Markus SCHMUDDE, Carsten KOBERG, Ralf KÜHN, Manuel GRAUPE
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Publication number: 20220090035Abstract: A method for modifying double stranded DNA (dsDNA) employing an RNA guided DNA endonuclease to generate two double strand breaks in the dsDNA molecule to be modified, and replacement of the sequence positioned between the double strand breaks with a substitute DNA sequence using the non-homologous end joining (NHEJ) pathway, and corresponding kits and compositions for modifying double stranded DNA molecules.Type: ApplicationFiled: December 21, 2018Publication date: March 24, 2022Inventors: Ralf KÜHN, Eric DANNER
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Patent number: 11273590Abstract: A starting-valve throttle device has a housing having a housing recess which is configured therein and in which a switching member is repositionable between an evacuation position and a delivery position. The switching member in the evacuation position configures an evacuation duct which connects an entry duct to an evacuation opening. The switching member in the delivery position configures a passage duct which connects the entry duct to a delivery duct. A throttle member is disposed in the passage duct so as to be repositionable relative to the switching member. On account thereof, a melt flowing through the passage duct is capable of being throttled in a desired manner. The starting-valve throttle device is constructed so as to be simple, compact and reliable. The operation of the starting-valve throttle device and an associated screw extruder is simple and energy-efficient.Type: GrantFiled: March 25, 2019Date of Patent: March 15, 2022Assignee: COPERION GMBHInventors: Michael Schmid, Stephan Kiern, Ralf Kühn, Markus Schmudde, Tomi Tratnik
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Publication number: 20220002757Abstract: The present invention relates to a nucleic acid molecule encoding (I) a polypeptide having the activity of an endonuclease, which is (a) a nucleic acid molecule encoding a polypeptide comprising or consisting of the amino acid sequence of SEQ ID NO: 1; (b) a nucleic acid molecule comprising or consisting of the nucleotide sequence of SEQ ID NO: 2; (c) a nucleic acid molecule encoding an endonuclease, the amino acid sequence of which is at least 70% identical to the amino acid sequence of SEQ ID NO: 1; (d) a nucleic acid molecule comprising or consisting of a nucleotide sequence which is at least 50% identical to the nucleotide sequence of SEQ ID NO: 2; (e) a nucleic acid molecule which is degenerate with respect to the nucleic acid molecule of (d); or (f) a nucleic acid molecule corresponding to the nucleic acid molecule of any one of (a) to (e) wherein T is replaced by U; (II) a fragment of the polypeptide of (I) having the activity of an endonuclease.Type: ApplicationFiled: September 22, 2021Publication date: January 6, 2022Inventor: Ralf KÜHN
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Patent number: 11149289Abstract: The present invention relates to a nucleic acid molecule encoding (I) a polypeptide having the activity of an endonuclease, which is (a) a nucleic acid molecule encoding a polypeptide comprising or consisting of the amino acid sequence of SEQ ID NO: 1; (b) a nucleic acid molecule comprising or consisting of the nucleotide sequence of SEQ ID NO: 2; (c) a nucleic acid molecule encoding an endonuclease, the amino acid sequence of which is at least 70% identical to the amino acid sequence of SEQ ID NO: 1; (d) a nucleic acid molecule comprising or consisting of a nucleotide sequence which is at least 50% identical to the nucleotide sequence of SEQ ID NO: 2; (e) a nucleic acid molecule which is degenerate with respect to the nucleic acid molecule of (d); or (f) a nucleic acid molecule corresponding to the nucleic acid molecule of any one of (a) to (e) wherein T is replaced by U; (II) a fragment of the polypeptide of (I) having the activity of an endonuclease.Type: GrantFiled: April 23, 2018Date of Patent: October 19, 2021Assignee: Helmholtz Zentrum München—Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH)Inventor: Ralf Kühn
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Publication number: 20190291326Abstract: A starting-valve throttle device has a housing having a housing recess which is configured therein and in which a switching member is repositionable between an evacuation position and a delivery position. The switching member in the evacuation position configures an evacuation duct which connects an entry duct to an evacuation opening. The switching member in the delivery position configures a passage duct which connects the entry duct to a delivery duct. A throttle member is disposed in the passage duct so as to be repositionable relative to the switching member. On account thereof, a melt flowing through the passage duct is capable of being throttled in a desired manner The starting-valve throttle device is constructed so as to be simple, compact and reliable. The operation of the starting-valve throttle device and an associated screw extruder is simple and energy-efficient.Type: ApplicationFiled: March 25, 2019Publication date: September 26, 2019Inventors: Michael SCHMID, Stephan KIERN, Ralf KÜHN, Markus SCHMUDDE, Tomi TRATNIK
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Patent number: 10214723Abstract: The present invention relates to a method of producing a non-human, mammalian oocyte carrying a modified target sequence in its genome, the method comprising the steps of introducing into a non-human, mammalian oocyte: (a) a clustered, regularly interspaced, short palindromic repeats (CRISPR)-associated protein 9 (Cas9 protein) or a nucleic acid molecule encoding said Cas9 protein; and (b-i) a target sequence specific CRISPR RNA (crRNA) and a trans-activating crRNA (tracr RNA) or a nucleic acid molecule encoding said RNAs; or (b-ii) a chimaeric RNA sequence comprising a target sequence specific crRNA and tracrRNA or a nucleic acid molecule encoding said RNA; wherein the Cas9 protein introduced in (a) and the RNA sequence(s) introduced in (b-i) or (b-ii) form a protein/RNA complex that specifically binds to the target sequence and introduces a single or double strand break within the target sequence.Type: GrantFiled: July 13, 2016Date of Patent: February 26, 2019Assignee: HELMHOLTZ ZENTRUM MÜNCHEN DEUTSCHES FORSCHUNGSZENTRUM FÜR GESUNDHEIT UND UMWELTInventors: Ralf Kühn, Wolfgang Wurst, Oskar Ortiz Sanchez
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Publication number: 20180237802Abstract: The present invention relates to a nucleic acid molecule encoding (I) a polypeptide having the activity of an endonuclease, which is (a) a nucleic acid molecule encoding a polypeptide comprising or consisting of the amino acid sequence of SEQ ID NO: 1; (b) a nucleic acid molecule comprising or consisting of the nucleotide sequence of SEQ ID NO: 2; (c) a nucleic acid molecule encoding an endonuclease, the amino acid sequence of which is at least 70% identical to the amino acid sequence of SEQ ID NO: 1; (d) a nucleic acid molecule comprising or consisting of a nucleotide sequence which is at least 50% identical to the nucleotide sequence of SEQ ID NO: 2; (e) a nucleic acid molecule which is degenerate with respect to the nucleic acid molecule of (d); or (f) a nucleic acid molecule corresponding to the nucleic acid molecule of any one of (a) to (e) wherein T is replaced by U; (II) a fragment of the polypeptide of (I) having the activity of an endonuclease.Type: ApplicationFiled: April 23, 2018Publication date: August 23, 2018Applicant: Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH)Inventor: Ralf KÜHN
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Patent number: 9855898Abstract: A plug-in system for holding at least one object in place in the interior of a motor vehicle includes a holding element for holding the object in place and a receiving element for mounting the holding element. The holding element can be connected with the receiving element by way of a releasable connection. In order to make available such a plug-in system, which is not susceptible to damage caused by overload or careless handling, an overload protection system is provided having a spherical element provided on the receiving element, a plate element that interacts with the spherical element, and a receiver for the spherical element of the deflectable receiving element, which receiver is configured on the plate element.Type: GrantFiled: March 19, 2015Date of Patent: January 2, 2018Assignee: Bayerische Motoren Werke AktiengesellschaftInventors: Ralf Kuhn, Eva-Maria Schemm, Carlos Chibante
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Patent number: 9783780Abstract: The present invention relates to a method of producing a non-human, mammalian oocyte carrying a modified target sequence in its genome, the method comprising the steps of introducing into a non-human, mammalian oocyte: (a) a clustered, regularly interspaced, short palindromic repeats (CRISPR)-associated protein 9 (Cas9 protein) or a nucleic acid molecule encoding said Cas9 protein; and (b-i) a target sequence specific CRISPR RNA (crRNA) and a trans-activating crRNA (tracr RNA) or a nucleic acid molecule encoding said RNAs; or (b-ii) a chimaeric RNA sequence comprising a target sequence specific crRNA and tracrRNA or a nucleic acid molecule encoding said RNA; wherein the Cas9 protein introduced in (a) and the RNA sequence(s) introduced in (b-i) or (b-ii) form a protein/RNA complex that specifically binds to the target sequence and introduces a single or double strand break within the target sequence.Type: GrantFiled: August 26, 2015Date of Patent: October 10, 2017Assignee: HELMHOLTZ ZENTRUM MÜNCHEN DEUTSCHES FORSCHUNGSZENTRUM FÜR GESUNDHEIT UND UMWELTInventors: Ralf Kühn, Wolfgang Wurst, Oskar Ortiz Sanchez
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Publication number: 20170175141Abstract: The present invention relates to a nucleic acid molecule encoding (I) a polypeptide having the activity of an endonuclease, which is (a) a nucleic acid molecule encoding a polypeptide comprising or consisting of the amino acid sequence of SEQ ID NO: 1; (b) a nucleic acid molecule comprising or consisting of the nucleotide sequence of SEQ ID NO: 2; (c) a nucleic acid molecule encoding an endonuclease, the amino acid sequence of which is at least 70% identical to the amino acid sequence of SEQ ID NO: 1; (d) a nucleic acid molecule comprising or consisting of a nucleotide sequence which is at least 50% identical to the nucleotide sequence of SEQ ID NO: 2; (e) a nucleic acid molecule which is degenerate with respect to the nucleic acid molecule of (d); or (f) a nucleic acid molecule corresponding to the nucleic acid molecule of any one of (a) to (e) wherein T is replaced by U; (II) a fragment of the polypeptide of (I) having the activity of an endonuclease.Type: ApplicationFiled: June 30, 2016Publication date: June 22, 2017Applicant: Helmholtz Zentrum Munchen - Deutsches Forschungszentrum fur Gesundheit und Umwelt (GmbH)Inventor: Ralf KÜHN
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Publication number: 20160319242Abstract: The present invention relates to a method of producing a non-human, mammalian oocyte carrying a modified target sequence in its genome, the method comprising the steps of introducing into a non-human, mammalian oocyte: (a) a clustered, regularly interspaced, short palindromic repeats (CRISPR)-associated protein 9 (Cas9 protein) or a nucleic acid molecule encoding said Cas9 protein; and (b-i) a target sequence specific CRISPR RNA (crRNA) and a trans-activating crRNA (tracr RNA) or a nucleic acid molecule encoding said RNAs; or (b-ii) a chimaeric RNA sequence comprising a target sequence specific crRNA and tracrRNA or a nucleic acid molecule encoding said RNA; wherein the Cas9 protein introduced in (a) and the RNA sequence(s) introduced in (b-i) or (b-ii) form a protein/RNA complex that specifically binds to the target sequence and introduces a single or double strand break within the target sequence.Type: ApplicationFiled: July 13, 2016Publication date: November 3, 2016Inventors: Ralf KÜHN, Wolfgang WURST, Oskar ORTIZ SANCHEZ
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Patent number: 9410134Abstract: The present invention relates to a nucleic acid molecule encoding (I) a polypeptide having the activity of an endonuclease, which is (a) a nucleic acid molecule encoding a polypeptide comprising or consisting of the amino acid sequence of SEQ ID NO: 1; (b) a nucleic acid molecule comprising or consisting of the nucleotide sequence of SEQ ID NO: 2; (c) a nucleic acid molecule encoding an endonuclease, the amino acid sequence of which is at least 70% identical to the amino acid sequence of SEQ ID NO: 1; (d) a nucleic acid molecule comprising or consisting of a nucleotide sequence which is at least 50% identical to the nucleotide sequence of SEQ ID NO: 2; (e) a nucleic acid molecule which is degenerate with respect to the nucleic acid molecule of (d); or (f) a nucleic acid molecule corresponding to the nucleic acid molecule of any one of (a) to (e) wherein T is replaced by U; (II) a fragment of the polypeptide of (I) having the activity of an endonuclease.Type: GrantFiled: June 6, 2012Date of Patent: August 9, 2016Assignee: Helmholtz Zentrum München—Deutsches Forschungszentrum für Gesundheit und UmweltInventor: Ralf Kühn
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Publication number: 20150376652Abstract: The present invention relates to a method of producing a non-human, mammalian oocyte carrying a modified target sequence in its genome, the method comprising the steps of introducing into a non-human, mammalian oocyte: (a) a clustered, regularly interspaced, short palindromic repeats (CRISPR)-associated protein 9 (Cas9 protein) or a nucleic acid molecule encoding said Cas9 protein; and (b-i) a target sequence specific CRISPR RNA (crRNA) and a trans-activating crRNA (tracr RNA) or a nucleic acid molecule encoding said RNAs; or (b-ii) a chimaeric RNA sequence comprising a target sequence specific crRNA and tracrRNA or a nucleic acid molecule encoding said RNA; wherein the Cas9 protein introduced in (a) and the RNA sequence(s) introduced in (b-i) or (b-ii) form a protein/RNA complex that specifically binds to the target sequence and introduces a single or double strand break within the target sequence.Type: ApplicationFiled: August 26, 2015Publication date: December 31, 2015Inventors: Ralf KÜHN, Wolfgang WURST, Oskar ORTIZ SANCHEZ
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Publication number: 20150191130Abstract: A plug-in system for holding at least one object in place in the interior of a motor vehicle includes a holding element for holding the object in place and a receiving element for mounting the holding element. The holding element can be connected with the receiving element by way of a releasable connection. In order to make available such a plug-in system, which is not susceptible to damage caused by overload or careless handling, an overload protection system is provided having a spherical element provided on the receiving element, a plate element that interacts with the spherical element, and a receiver for the spherical element of the deflectable receiving element, which receiver is configured on the plate element.Type: ApplicationFiled: March 19, 2015Publication date: July 9, 2015Inventors: Ralf KUHN, Eva-Maria SCHEMM, Carlos CHIBANTE
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Publication number: 20140304847Abstract: The present invention relates to a method for modifying a target sequence in the genome of a mammalian cell, the method comprising the step of introducing into a mammalian cell: a. one or more compounds that introduce double-strand breaks in said target sequence; b. one or more DNA molecules comprising a donor DNA sequence to be incorporated by homologous recombination into the genomic DNA of said mammalian cell within said target sequence, wherein said donor DNA sequence is flanked upstream by a first flanking element and downstream by a second flanking element, wherein said first and second flanking element are different and wherein each of said first and second flanking sequence are homologous to a continuous DNA sequence on either side of the double-strand break introduced by said one or more compounds of a. within said target sequence in the genome of said mammalian cell; and c.Type: ApplicationFiled: June 6, 2012Publication date: October 9, 2014Inventors: Ralf Kühn, Wolfgang Wurst
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Publication number: 20140298505Abstract: The present invention relates to a nucleic acid molecule encoding (I) a polypeptide having the activity of an endonuclease, which is (a) a nucleic acid molecule encoding a polypeptide comprising or consisting of the amino acid sequence of SEQ ID NO: 1; (b) a nucleic acid molecule comprising or consisting of the nucleotide sequence of SEQ ID NO: 2; (c) a nucleic acid molecule encoding an endonuclease, the amino acid sequence of which is at least 70% identical to the amino acid sequence of SEQ ID NO: 1; (d) a nucleic acid molecule comprising or consisting of a nucleotide sequence which is at least 50% identical to the nucleotide sequence of SEQ ID NO: 2; (e) a nucleic acid molecule which is degenerate with respect to the nucleic acid molecule of (d); or (f) a nucleic acid molecule corresponding to the nucleic acid molecule of any one of (a) to (e) wherein T is replaced by U; (II) a fragment of the polypeptide of (I) having the activity of an endonuclease.Type: ApplicationFiled: June 6, 2012Publication date: October 2, 2014Inventor: Ralf Kühn