Abstract: The invention concerns a method for isolating and purifying nucleic acids from a sample and a device that is suitable therefore.

Abstract: The invention concerns a method for isolating and purifying nucleic acids from a sample and a device that is suitable therefore.

Abstract: The invention concerns a method for isolating and purifying nucleic acids from a sample and a device that is suitable therefore.

Abstract: The invention concerns a method for isolating and purifying nucleic acids from a sample and a device that is suitable therefore.

Abstract: The invention concerns a method for isolating and purifying nucleic acids from a sample and a device that is suitable therefore.

Abstract: The present invention is directed to a method for adsorbing, i.e. non-covalently binding, nucleic acids to a solid phase using a two-step procedure. Furthermore, the present invention pertains to a method for isolating nucleic acids from a biological sample. In the first step of the procedure, lysis is effected by mixing the biological sample with an aqueous lysis buffer containing a chaotropic agent and incubating the mixture; in the second step, the concentration of the chaotropic agent in the mixture is increased and the mixture is contacted with the solid phase, whereby the nucleic acids in the liquid phase is adsorbed to the solid phase.

Abstract: A method for purification of a nucleic acid comprising the steps of (a) adsorbing on a substrate the nucleic acid from a composition containing (i) an aqueous buffer, (ii) salts in a high concentration, (iii) a water-miscible, non-acidic organic compound, and (iv) the nucleic acid; (b) optionally washing with a washing solution the substrate with the adsorbed nucleic acid; (c) contacting the substrate with the adsorbed nucleic acid with a solution containing salts in a lower concentration compared to the composition of step (a), thereby desorbing the nucleic acid from the substrate; (d) separating the solution with the desorbed nucleic acid from the substrate, thereby purifying the nucleic acid; and optionally (e) precipitating the desorbed nucleic acid from the solution of step (d) and isolating the precipitated nucleic acid, thereby further purifying the nucleic acid.

Abstract: Stabilized aqueous solution of a coenzyme for hydrogen-transferring enzymes characterized in that the solution contains NAD, NADP or a derivative thereof in an oxidized or reduced form and one or several organic compounds or salts thereof having a pKa value between 1.5 and 6.0 and/or a nitrogen compound of the general formula in which the residues R1, R2 and R3 are the same or different and denote hydrogen, or a saturated or unsaturated alkyl or aryl group as well as the use of the solution to determine dehydrogenases in particular lactate dehydrogenase or substrates thereof.

Abstract: The present invention is directed to a method for adsorbing, i.e. non-covalently binding, nucleic acids to a solid phase using a two-step procedure. Furthermore, the present invention pertains to a method for isolating nucleic acids from a biological sample. In the first step of the procedure, lysis is effected by mixing the biological sample with an aequous lysis buffer containing a chaotropic agent and incubating the mixture; in the second step, the concentration of the chaotropic agent in the mixture is increased and the mixture is contacted with the solid phase, whereby the nucleic acids in the liquid phase is adsorbed to the solid phase.

Abstract: The invention concerns a method for isolating and purifying nucleic acids from a sample and a device that is suitable therefore.

Abstract: The present invention is directed to methods and kits for isolation of bacteria from biological samples. In such methods antibodies specific for eukaryotic cells, which are deficient in a bacteria-binding Fc-terminus, are used for separating eukaryotic cells from biological samples.

Abstract: Stabilized aqueous solution of a coenzyme for hydrogen-transferring enzymes characterized in that the solution contains NAD, NADP or a derivative thereof in an oxidized or reduced form and one or several organic compounds or salts thereof having a pKa value between 1.5 and 6.0 and/or a nitrogen compound of the general formula in which the residues R1, R2 and R3 are the same or different and denote hydrogen, or a saturated or unsaturated alkyl or aryl group as well as the use of the solution to determine dehydrogenases in particular lactate dehydrogenase or substrates thereof.

Abstract: A method for purification of a nucleic acid comprising the steps of (a) adsorbing on a substrate the nucleic acid from a composition containing (i) an aqueous buffer, (ii) salts in a high concentration, (iii) a water-miscible, non-acidic organic compound, and (iv) the nucleic acid; ( b) optionally washing with a washing solution the substrate with the adsorbed nucleic acid; (c) contacting the substrate with the adsorbed nucleic acid with a solution containing salts in a lower concentration compared to the composition of step (a), thereby desorbing the nucleic acid from the substrate; (d) separating the solution with the desorbed nucleic acid from the substrate, thereby purifying the nucleic acid; and optionally (e) precipitating the desorbed nucleic acid from the solution of step (d) and isolating the precipitated nucleic acid, thereby further purifying the nucleic acid.

Abstract: The invention concerns a method and reagent for the determination of lipase in the presence of a colour substrate and at least one N-substituted carboxylic acid amide derivative or a compound of the general formula (I) or (Ia) in which R1 and R2 independently of one another represent hydrogen or a saturated or unsaturated, substituted or unsubstituted hydrocarbon residue with 2 to 24 carbon atoms, Z denotes a saturated or unsaturated, substituted, cyclic or straight-chained hydrocarbon residue with 1 to 10 carbon atoms, X represents an atom or a group of atoms with positive charge and n is a number from 1 to 3. Tetra-sodium-N-(1,2-dicarboxylethyl)-N-alkylsulfosuccinamide or a mixture containing such a compound has proven to be particularly advantageous for the elimination of unspecific reactions in the determination of lipase in a biological sample.

Abstract: Stabilized aqueous solution of a coenzyme for hydrogen-transferring enzymes characterized in that the solution contains NAD, NADP or a derivative thereof in an oxidized or reduced form and one or several organic compounds or salts thereof having a pKa value between 1.5 and 6.

Abstract: Stabilized aqueous coenzyme solutions which contain up to ca. 60 mM of a heavy metal salt and optionally a complexing agent in a defined ratio to the heavy metal salt as well as their use for the determination of dehydrogenases in particular lactate dehydrogenase or corresponding substrates in an alkaline environment.