Abstract: The present invention is directed to a method, i.e. an immunoassay, for determining the presence and/or the amount of anti-zika Anti-ZIKV #1 virus antibodies, i.e. zika virus-specific antibodies in a sample. Therefore, the present invention is directed to a microsphere complex comprising a microsphere coupled to a zika virus like particle, as well as to a kit comprising said microsphere complex and an amount of reporter antibody that binds to the zika virus like particle. The present invention further relates to a method for determining an antibody correlate of protection against zika virus infection for a zika virus vaccine. Moreover, the present invention is directed to a method for diagnosing the protection of a human or non-human subject against a zika virus infection.

Abstract: A geodesic positioning system determines the position of a target point. The system includes a range pole that can be oriented toward the target point. A tilt sensor is arranged on the range pole, detachably where applicable, for generating a tilt angle signal related to the tilt angle of the range pole. A positioning element, more particularly a reflector or a satellite signal antenna, is arranged on the range pole. Positioning means is able to capture the position of the positioning element as a function of the tilt angle signal. Triggering means is in signal communication with the tilt sensor and with the positioning means, and adapted so that a trigger signal issued to capture the current position of the positioning element is transmitted to the positioning means as soon as the tilt angle signal corresponds to a tilt angle situated within a given tilt angle range.

Abstract: A geodesic positioning system determines the position of a target point. The system includes a range pole that can be oriented toward the target point. A tilt sensor is arranged on the range pole, detachably where applicable, for generating a tilt angle signal related to the tilt angle of the range pole. A positioning element, more particularly a reflector or a satellite signal antenna, is arranged on the range pole. Positioning means is able to capture the position of the positioning element as a function of the tilt angle signal. Triggering means is in signal communication with the tilt sensor and with the positioning means, and adapted so that a trigger signal issued to capture the current position of the positioning element is transmitted to the positioning means as soon as the tilt angle signal corresponds to a tilt angle situated within a given tilt angle range.

Abstract: Methods of eliciting an immune response in a subject by administering one or more large genomic DNA fragments are provided. Also provided are methods of identifying sequences encoding antigenic polypeptides. Also provided are vaccine compositions comprising one or more large genomic DNA fragments.

Abstract: The invention relates to a method of eliciting a T cell response against a T cell epitope in a host mammalian subject, which method comprises: (i) a first immunisation that comprises at least two administrations which are from 1 to 14 days apart to the subject, wherein each administration comprises administering a nucleotide of interest (NOI) encoding the T cell epitope, and optionally (ii) a second immunisation that comprises at least one administration to the subject of (a) a NOI encoding the T cell epitope, or (b) a protein comprising the T cell epitope, wherein the time between the first administration of the first immunisation, and the first administration of the second immunisation, is from 21 to 365 days.

Abstract: The invention relates to the fields of vaccines, vaccine adjuvants, molecular biology and immunology, and generally relates to adjuvants and nucleic acid immunization techniques. More specifically, the invention relates to certain adjuvant compositions, and to vaccine and/or nucleic acid immunization strategies employing such compositions.

Abstract: Nucleic acid constructs comprising viral genomic nucleic acid which comprises at least two endogenous gene expression regulatory units which each comprise an endogenous promoter where the endogenous promoters of the units are active at the same phase in the viral life cycle of the virus the viral genomic nucleic acid is derived from are provided. The endogenous gene expression regulatory units are used to express particular chosen heterologous coding sequences and in particular to express heterologous antigens. The constructs can be used in a vaccine to generate an immune response against the heterologous antigens and in particular may be used in a DNA vaccine. Methods for generating the constructs and means for their administration are also provided.

Abstract: The relates to certain adjuvant compositions, and to vaccine and/or nucleic acid immunization strategies employing such compositions. The invention in particular relates to DNA vaccines that are useful in the prophylaxis and treatment of HIV infections, more particularly when administered by particle mediated delivery.

Abstract: The present invention relates to the fields of vaccines, vaccine adjuvants, molecular biology and immunology, and generally adjuvants and nucleic acid immunization techniques. More specifically, the invention relates to certain adjuvant compositions, and to vaccine and/or nucleic acid immunization strategies employing such compositions. The invention in particular relates to DNA vaccines that are useful in the prophylaxis and treatment of HIV infections, more particularly when administered by particle mediated delivery.