Abstract: Systems and methods for determining bacterial load in targets are disclosed. An autofluorescence detection and collection device includes a light source configured to illuminate a target with excitation light causing at least one biomarker in the illuminated target to fluoresce. Bacterial autofluorescence data regarding the target is collected and analyzed to determine bacterial load the target. The autofluorescence data may be analyzed using pixel intensity.

Abstract: An adaptor for configuring a mobile communication device for tissue imaging is disclosed. The adaptor includes a housing configured to be removably coupled to a mobile communication device, at least one excitation light source for fluorescent imaging, a white light source for white light imaging, and a power source for powering the adaptor. The excitation light source is configured to emit light in one of ultraviolet, visible, near-infrared, and infrared ranges.

Abstract: A system for determining a bacterial load of a target is provided. The system includes an adaptor for configuring a mobile communication device for tissue imaging and a mobile communication device. The adaptor includes a housing configured to be removably coupled to a mobile communication device and, an excitation light source for fluorescent imaging. The excitation light source is configured to emit light in one of ultraviolet, visible, near-infrared, and infrared ranges.

Abstract: Given a specific imaging device and systems further described herein, wound characteristics of a wound fluoresce with a unique spectral signature when subjected to excitation light with a known wavelength or range of wavelengths. Images captured therefrom are subject to analyses by one or more analysis modules trained using one or more of reflectance images and/or data, fluorescence images and/or data, RGB image data, RGB color data, and wound characteristics data. Wound sizes, boundaries, bacterial presence, and other characteristics may be quantified and graphically represented as an overlay on the original wound image along with documentation related to the wound. Reflectance, fluorescent, and thermal images of the wound may be acquired, subject to analyses with the trained analysis modules, and two or more of the analyzed images may be overlaid to form a composite image upon which areas of interest from each image is highlighted or otherwise indicated.

Abstract: Systems and methods for determining bacterial load in targets are disclosed. An autofluorescence detection and collection device includes a light source configured to illuminate a target with excitation light causing at least one biomarker in the illuminated target to fluoresce. Bacterial autofluorescence data regarding the target is collected and analyzed to determine bacterial load the target. The autofluorescence data may be analyzed using pixel intensity.

Abstract: Given a specific imaging device and systems further described herein, wound characteristics of a wound fluoresce with a unique spectral signature when subjected to excitation light with a known wavelength or range of wavelengths. Images captured therefrom are subject to analyses of pixels thereof, with a plurality of training images having known wound sizes and characteristics marked-up thereon being used to generate training data, which is subsequently used to identify wound characteristics from test images in real time. Wound sizes, boundaries, bacterial presence, and other characteristics may be quantified and graphically represented as an overlay on the original wound image along with documentation related to the wound.

Abstract: An adaptor for configuring a mobile communication device for tissue imaging is disclosed. The adaptor includes a housing configured to be removably coupled to a mobile communication device, at least one excitation light source for fluorescent imaging, a white light source for white light imaging, and a power source for powering the adaptor. The excitation light source is configured to emit light in one of ultraviolet, visible, near-infrared, and infrared ranges.

Abstract: Systems and methods for obtaining diagnostic data of a target are disclosed. The systems include an imaging device and a drape. The imaging device includes at least one excitation light source for fluorescent imaging, and an optical sensor configured to detect signals responsive to illumination of the target with the excitation light. The drape is configured to reduce ambient light surrounding the target during imaging.

Abstract: A portable, handheld device for fluorescence-based imaging is provided. The device comprises a wireless communication device having a sensor configured to detect optical signals. The device further comprises an assembly configured to receive and secure the wireless communication device therein. The assembly includes a housing, at least one light source coupled to the housing, a power supply, and an optical filter holder coupled to the housing and configured to receive one or more optical filters. An endoscope portion of the device is positioned relative to the sensor to visualize at least a portion of a confined anatomical space and to receive optical signals from a visualized, illuminated portion of a target positioned within the confined anatomical space. A processor of the device includes image analysis software and is configured to produce a composite representation of the illuminated portion of the target positioned within the confined anatomical space.

Abstract: A drape for use with fluorescence-based imaging and white-light imaging includes a drape body. The drape body is configured to limit passage of electromagnetic radiation including light through the drape body to an interior imaging environment defined by the drape body such that electromagnetic radiation including ambient light within the interior imaging environment does not exceed a predetermined threshold. The drape also includes a connecting element permanently coupled to the drape body. The connecting element defines a hole in the drape body. The connecting element is configured to attach the drape to a portable, handheld imaging device.

Abstract: A method of assessing surgical margins is disclosed. The method includes, subsequent to administration of a compound configured to induce emissions of between about 600 nm and about 660 nm in cancerous tissue cells, positioning a distal end of a handheld, white light and fluorescence-based imaging device adjacent to a surgical margin. The method also includes, with the handheld device, substantially simultaneously exciting and detecting autofluorescence emissions of tissue cells and fluorescence emissions of the induced wavelength in tissue cells of the surgical margin. And, based on a presence or an amount of fluorescence emissions of the induced wavelength detected in the tissue cells of the surgical margin, determining whether the surgical margin is substantially free of at least one of precancerous cells, cancerous cells, and satellite lesions. The compound may be a non-activated, non-targeted compound such as ALA.

Abstract: Given a specific imaging device and systems further described herein, wound characteristics of a wound fluoresce with a unique spectral signature when subjected to excitation light with a known wavelength or range of wavelengths. Images captured therefrom are subject to analyses of pixels thereof, with a plurality of training images having known wound sizes and characteristics marked-up thereon being used to generate training data, which is subsequently used to identify wound characteristics from test images in real time. Wound sizes, boundaries, bacterial presence, and other characteristics may be quantified and graphically represented as an overlay on the original wound image along with documentation related to the wound.

Abstract: Disclosed herein are multifunctional nanoparticle compositions. The compositions can be useful for the treatment of cancer by enhancing the anti-tumor effectiveness of radiation directed to a tissue, cell or a tumor and the methods of use thereof. The multifunctional nanoparticle composition comprises a metal oxide nanoparticle core; a functional coating on the surface of the metal oxide nanoparticle core; and a matrix carrier in which the coated nanoparticle is embedded.

Abstract: Systems and methods for obtaining diagnostic data of a target are disclosed. The systems include an imaging device and a drape. The imaging device includes at least one excitation light source for fluorescent imaging, and an optical sensor configured to detect signals responsive to illumination of the target with the excitation light. The drape is configured to reduce ambient light surrounding the target during imaging.

Abstract: Systems and methods for determining bacterial load in targets and tracking changes in bacterial load of targets over time are disclosed. An autofluorescence detection and collection device includes a light source configured to directly illuminate at least a portion of a target and an area around the target with excitation light causing at least one biomarker in the illuminated target to fluoresce. Bacterial autofluorescence data regarding the illuminated portion of the target and the area around the target is collected and analyzed to determine bacterial load of the illuminated portion of the target and area around the target. The autofluorescence data may be analyzed using pixel intensity. Changes in bacterial load of the target over time may be tracked. The target may be a wound in tissue.