Abstract: The present disclosure provides tobacco inbred plants K326 SRC, CMS K326 SRC, K346 SRC, CMS K346 SRC, NC1562-1 SRC, NCTG-61 SRC, CMS NCTG-61 SRC and hybrid NC196 SRC. The present disclosure also provides parts of such plants and products made from those parts. The present disclosure also includes progeny of the provided plants including hybrids.

Abstract: The present disclosure provides tobacco inbred plants Banket A1 SRC, CMS Banket A1 SRC, NC 2000 SRC, CMS NC 2000 SRC, NC 2002 SRC, CMS NC 2002 SRC, Burley 21 SRC, and CMS Burley 21 SRC. The present disclosure also provides parts of such plants and cured tobacco and tobacco products made from those parts. The present disclosure also includes progenies of the provided plants including hybrids.

Abstract: The present disclosure provides tobacco inbred plants NC174 SRC, CMS NC174 SRC, NC821-11 SRC, NC775 Bmr/Bmr SRC, CMS NC775 Bmr/Bmr SRC, NC645 Bmr/Bmr SRC, and hybrids NC 5 SRC and NC 8 SRC. The present disclosure also provides parts of such plants and cured tobacco and tobacco products made from those parts. The present disclosure also includes progenies of the provided plants including hybrids.

Abstract: The present disclosure provides tobacco inbred plants NC1209-23 SRC, CMS NC1209-23 SRC, DH19 SRC, DH98-325-5 SRC, NC1426-11 SRC, CMS NC1426-11 SRC, and NC1426-17 SRC, and hybrids NC 3 SRC, NC 6 SRC, and NC 4 SRC. The present disclosure also provides parts of such plants, cured tobacco, and tobacco products made from those parts. The present disclosure also includes progenies of the provided plants including hybrids.

Abstract: The present disclosure provides tobacco inbred plants TN90 SRC, CMS TN90 SRC, KY14 SRC, CMS KY14 SRC, L8 SRC, NC775 SRC, CMS NC775 SRC, NC645 SRC, NC638 SRC, CMS NC638 SRC, TN86 SRC, and CMS TN86 SRC, and hybrids KY14×L8 SRC, NC7 SRC, and NCBH129 SRC. The present disclosure also provides parts of such plants and products made from those parts. The present disclosure also includes progeny of the provided plants including hybrids.

Abstract: Compositions and methods for reducing the level of nornicotine and N?-nitrosonornicotine (NNN) in tobacco plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for a root-specific nicotine demethylases, CYP82E10, and variants thereof, that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Compositions of the invention also include tobacco plants, or plant parts thereof, comprising a mutation in a gene encoding a CYP82E10 nicotine demethylase, wherein the mutation results in reduced expression or function of the CYP82E10 nicotine demethylase. Seed of these tobacco plants, or progeny thereof, and tobacco products prepared from the tobacco plants of the invention, or from plant parts or progeny thereof, are also provided. Methods for reducing the level of nornicotine, or reducing the rate of conversion of nicotine to nornicotine, in a tobacco plant, or plant part thereof are also provided.

Abstract: Compositions and methods for reducing the level of nornicotine and N?-nitrosonornicotine (NNN) in Nicotiana plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for cytochrome P450s that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Expression cassettes, vectors, plants, and plant parts thereof comprising inhibitory sequences that target expression or function of the disclosed cytochrome P450 polypeptides are also provided. Methods for the use of these novel sequences to inhibit expression or function of cytochrome P450 polypeptides involved in this metabolic conversion are also provided. The methods find use in the production of tobacco products that have reduced levels of nornicotine and its carcinogenic metabolite, NNN, and thus reduced carcinogenic potential for individuals consuming these tobacco products or exposed to secondary smoke derived from these products.

Abstract: Compositions and methods for reducing the level of nornicotine and N?-nitrosonornicotine (NNN) in Nicotiana plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for cytochrome P450s that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Expression cassettes, vectors, plants, and plant parts thereof comprising inhibitory sequences that target expression or function of the disclosed cytochrome P450 polypeptides are also provided. Methods for the use of these novel sequences to inhibit expression or function of cytochrome P450 polypeptides involved in this metabolic conversion are also provided. The methods find use in the production of tobacco products that have reduced levels of nornicotine and its carcinogenic metabolite, NNN, and thus reduced carcinogenic potential for individuals consuming these tobacco products or exposed to secondary smoke derived from these products.

Abstract: Compositions and methods for reducing the level of nornicotine and N?-nitrosonornicotine (NNN) in tobacco plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for a root-specific nicotine demethylases, CYP82E10, and variants thereof, that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Compositions of the invention also include tobacco plants, or plant parts thereof, comprising a mutation in a gene encoding a CYP82E10 nicotine demethylase, wherein the mutation results in reduced expression or function of the CYP82E10 nicotine demethylase. Seed of these tobacco plants, or progeny thereof, and tobacco products prepared from the tobacco plants of the invention, or from plant parts or progeny thereof, are also provided. Methods for reducing the level of nornicotine, or reducing the rate of conversion of nicotine to nornicotine, in a tobacco plant, or plant part thereof are also provided.

Abstract: Compositions and methods for reducing the level of nornicotine and N?-nitrosonornicotine (NNN) in Nicotiana plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for cytochrome P450s that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Expression cassettes, vectors, plants, and plant parts thereof comprising inhibitory sequences that target expression or function of the disclosed cytochrome P450 polypeptides are also provided. Methods for the use of these novel sequences to inhibit expression or function of cytochrome P450 polypeptides involved in this metabolic conversion are also provided. The methods find use in the production of tobacco products that have reduced levels of nornicotine and its carcinogenic metabolite, NNN, and thus reduced carcinogenic potential for individuals consuming these tobacco products or exposed to secondary smoke derived from these products.

Abstract: Compositions and methods for reducing the level of nornicotine and N?-nitrosonornicotine (NNN) in Nicotiana plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for cytochrome P450s that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Expression cassettes, vectors, plants, and plant parts thereof comprising inhibitory sequences that target expression or function of the disclosed cytochrome P450 polypeptides are also provided. Methods for the use of these novel sequences to inhibit expression or function of cytochrome P450 polypeptides involved in this metabolic conversion are also provided. The methods find use in the production of tobacco products that have reduced levels of nornicotine and its carcinogenic metabolite, NNN, and thus reduced carcinogenic potential for individuals consuming these tobacco products or exposed to secondary smoke derived from these products.

Abstract: Compositions and methods for reducing the level of nornicotine and N?-nitrosonornicotine (NNN) in Nicotiana plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for cytochrome P450s that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Expression cassettes, vectors, plants, and plant parts thereof comprising inhibitory sequences that target expression or function of the disclosed cytochrome P450 polypeptides are also provided. Methods for the use of these novel sequences to inhibit expression or function of cytochrome P450 polypeptides involved in this metabolic conversion are also provided. The methods find use in the production of tobacco products that have reduced levels of nornicotine and its carcinogenic metabolite, NNN, and thus reduced carcinogenic potential for individuals consuming these tobacco products or exposed to secondary smoke derived from these products.

Abstract: The present disclosure provides tobacco inbred plants Banket A1 SRC, CMS Banket A1 SRC, NC 2000 SRC, CMS NC 2000 SRC, NC 2002 SRC, CMS NC 2002 SRC, Burley 21 SRC, and CMS Burley 21 SRC. The present disclosure also provides parts of such plants and cured tobacco and tobacco products made from those parts. The present disclosure also includes progenies of the provided plants including hybrids.

Abstract: The present disclosure provides tobacco inbred plants NC1209-23 SRC, CMS NC1209-23 SRC, DH19 SRC, DH98-325-5 SRC, NC1426-11 SRC, CMS NC1426-11 SRC, and NC1426-17 SRC, and hybrids NC 3 SRC, NC 6 SRC, and NC 4 SRC. The present disclosure also provides parts of such plants, cured tobacco, and tobacco products made from those parts. The present disclosure also includes progenies of the provided plants including hybrids.

Abstract: The present disclosure provides tobacco inbred plants NC174 SRC, CMS NC174 SRC, NC821-11 SRC, NC775 Bmr/Bmr SRC, CMS NC775 Bmr/Bmr SRC, NC645 Bmr/Bmr SRC, and hybrids NC 5 SRC and NC 8 SRC. The present disclosure also provides parts of such plants and cured tobacco and tobacco products made from those parts. The present disclosure also includes progenies of the provided plants including hybrids.

Abstract: The present invention provides tobacco inbred plants NCBEX1F and NCBEX1MS, and NC EX90. The present invention also provides parts of such plants and products made from those parts. The present invention also includes progeny of the provided plants including hybrids.

Abstract: The present disclosure provides tobacco inbred plants TN90 SRC, CMS TN90 SRC, KY14 SRC, CMS KY14 SRC, L8 SRC, NC775 SRC, CMS NC775 SRC, NC645 SRC, NC638 SRC, CMS NC638 SRC, TN86 SRC, and CMS TN86 SRC, and hybrids KY14 X L8 SRC, NC7 SRC, and NCBH129 SRC. The present disclosure also provides parts of such plants and products made from those parts. The present disclosure also includes progeny of the provided plants including hybrids.

Abstract: The present disclosure provides tobacco inbred plants K326 SRC, CMS K326 SRC, K346 SRC, CMS K346 SRC, NC1562-1 SRC, NCTG-61 SRC, CMS NCTG-61 SRC and hybrid NC196 SRC. The present disclosure also provides parts of such plants and products made from those parts. The present disclosure also includes progeny of the provided plants including hybrids.

Abstract: The present invention relates to a method of Agrobaterium-based transformation of a plant comprising: generating a callus explant by, performing callus induction and maintenance in a plant in at least a first solution comprising less than about 100 mg/l of inositol; subjecting the callus explant to a cold treatment in a second solution comprising glutamine; and co-cultivating the callus explant with an Agrobacterium in a third solution comprising glutamine. The present invention also relates to a callus induction medium and/or callus maintenance medium, comprising: less than about 100 mg/l of inositol, and optionally at least one constituent selected from the group consisting of: an N6 salt or any plant culture media, proline, casein hydrolysate, a B-Vitamin, maltose, phytagel, 2,4-D, BAP and/or other plant growth regulators/hormones.

Abstract: Compositions and methods for reducing the level of nornicotine and N?-nitrosonornicotine (NNN) in tobacco plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for a root-specific nicotine demethylases, CYP82E10, and variants thereof, that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Compositions of the invention also include tobacco plants, or plant parts thereof, comprising a mutation in a gene encoding a CYP82E10 nicotine demethylase, wherein the mutation results in reduced expression or function of the CYP82E10 nicotine demethylase. Seed of these tobacco plants, or progeny thereof, and tobacco products prepared from the tobacco plants of the invention, or from plant parts or progeny thereof, are also provided. Methods for reducing the level of nornicotine, or reducing the rate of conversion of nicotine to nornicotine, in a tobacco plant, or plant part thereof are also provided.