Patents by Inventor Ralph Weissleder

Ralph Weissleder has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • FUNCTIONALIZED 1,2,4,5-TETRAZINE COMPOUNDS FOR USE IN BIOORTHOGONAL COUPLING REACTIONS
    Publication number: 20150246893
    Abstract: The present application relates to functionalized 1,2,4,5-tetrazine compounds. The compounds are useful in compositions and methods using bioorthogonal inverse electron demand Diels-Alder cycloaddition reactions for the rapid and specific covalent delivery of a “payload” to a ligand bound to a biological target.
    Type: Application
    Filed: March 14, 2013
    Publication date: September 3, 2015
    Applicant: The General Hospital Corporation
    Inventors: Neal K. Devaraj, Mark R. Karver, Scott A. Hilderbrand, Ralph Weissleder
  • Magnetic resonance-based viscometers and methods
    Patent number: 9097644
    Abstract: This invention relates generally to magnetic resonance (MR)-based methods and kits for measuring the viscosity of liquid samples.
    Type: Grant
    Filed: August 15, 2008
    Date of Patent: August 4, 2015
    Assignees: Massachusetts Institute of Technology, The General Hospital Corporation
    Inventors: Lee Josephson, Rui Hong, Michael J. Cima, Ralph Weissleder
  • Plectin-1 Targeted Agents for Detection and Treatment of Pancreatic Ductal Adenocarcinoma
    Publication number: 20150151010
    Abstract: Described herein are compositions and methods for cancer cell biomarkers, such as pancreatic ductal adenocarcinoma (PDAC) cell biomarkers, and binding molecules for diagnosis and treatment of cancer, e.g., PDAC. Methods of identifying “accessible” proteomes are disclosed for identifying cancer biomarkers, such as plectin-1, a PDAC biomarker. Additionally, imaging compositions are provided comprising magnetofluorescent nanoparticles conjugated to peptide ligands for identifying PDACs.
    Type: Application
    Filed: September 8, 2014
    Publication date: June 4, 2015
    Inventors: Kimberly Kelly, Ralph Weissleder, Nabeel Bardeesy
  • Magnetic Labeling of Bacteria
    Publication number: 20150125884
    Abstract: The present invention provides novel methods of magnetically labeling a bacterial cell by contacting the call with an affinity ligand and subsequently contacting the cell with a magnetic agent, where the affinity ligand and magnetic agent include bioorthogonally reactive groups that can react with each other to form a covalent bond. Compounds, compositions, kits and applications of the method are also described.
    Type: Application
    Filed: March 8, 2013
    Publication date: May 7, 2015
    Inventors: Ghyslain Budin, Ralph Weissleder, Hakho Lee, Hyun Jung Chung
  • PHOTOACTIVATABLE CAGED TAMOXIFEN AND TAMOXIFEN DERIVATIVE MOLECULES AND METHODS OF USE THEREOF
    Publication number: 20150093365
    Abstract: Provided herein are compositions containing photoactivatable caged tamoxifen and tamoxifen derivative molecules. Also provided are kits containing one of these compositions and a light source. Also provided are methods of optically inducing nuclear translocation of a fusion protein containing a mammalian estrogen receptor ligand binding domain in a eukaryotic cell and methods of optically inducing recombination in a eukaryotic cell that include contacting a eukaryotic cell with at least one of these compositions. Also provided are methods of treating breast cancer in a subject that include administering a photoactivatable caged tamoxifen or tamoxifen derivative molecule to a subject having breast cancer.
    Type: Application
    Filed: March 14, 2013
    Publication date: April 2, 2015
    Inventors: Xin Lu, Sarit S. Agasti, Ronald A. DePinho, Ralph Weissleder
  • Magnetic nanoparticles
    Patent number: 8945628
    Abstract: A magnetic nanoparticle includes a magnetic core and a superparamagnetic outer shell, in which the outer shell enhances magnetic properties of the nanoparticle. The enhanced magnetic properties of the magnetic nanoparticle allow for highly sensitive detection as well as diminished non-specific aggregation of nanoparticles.
    Type: Grant
    Filed: May 26, 2011
    Date of Patent: February 3, 2015
    Assignee: The General Hospital Corporation
    Inventors: Ralph Weissleder, Hakho Lee, Tae-Jong Yoon
  • Monofunctional carbocyanine dyes for in vivo and in vitro imaging
    Patent number: 8916137
    Abstract: The present invention provides symmetric carbocyanine dyes and dye precursors useful for fluorescence microscopy, and methods of making and using same.
    Type: Grant
    Filed: November 9, 2009
    Date of Patent: December 23, 2014
    Assignee: The General Hospital Corporation
    Inventors: Scott A. Hilderbrand, Ralph Weissleder, Fangwei Shao
  • Compositions and methods for delivering a substance to a biological target
    Patent number: 8900549
    Abstract: The present application provides compositions and methods using bioorthogonal inverse electron demand Diels-Alder cycloaddition reaction for rapid and specific covalent delivery of a “payload” to a ligand bound to a biological target.
    Type: Grant
    Filed: November 2, 2009
    Date of Patent: December 2, 2014
    Assignee: The General Hospital Corporation
    Inventors: Scott A. Hilderbrand, Neal K. Devaraj, Ralph Weissleder
  • Magnetic-nanoparticle conjugates and methods of use
    Publication number: 20140303022
    Abstract: The present invention provides novel compositions of binding moiety-nanoparticle conjugates, aggregates of these conjugates, and novel methods of using these conjugates, and aggregates. The nanoparticles in these conjugates can be magnetic metal oxides, either monodisperse or polydisperse. Binding moieties can be, e.g., oligonucleotides, polypeptides, or polysaccharides. Oligonucleotide sequences are linked to either non-polymer surface functionalized metal oxides or with functionalized polymers associated with the metal oxides. The novel compositions can be used in assays for detecting target molecules, such as nucleic acids and proteins, in vitro or as magnetic resonance (MR) contrast agents to detect target molecules in living organisms.
    Type: Application
    Filed: April 18, 2014
    Publication date: October 9, 2014
    Applicant: The General Hospital Corporation
    Inventors: Lee Josephson, Ralph Weissleder, J. Manuel Perez
  • Analyte Detection Using Magnetic Hall Effect
    Publication number: 20140295460
    Abstract: Determining a presence of a target analyte in a fluid sample includes mixing multiple magnetic particles with the fluid sample, in which the magnetic particles are each bound to one or more binding moieties that specifically bind to the target analyte, flowing the fluid sample containing the magnetic particles through a fluidic channel, exposing the fluid sample in the fluidic channel to a magnetic field, measuring a signal from a Hall effect sensor while the fluid sample flows through the fluidic channel, and determining whether the target analyte is present in the fluid sample when the measured signal is in a first range of values.
    Type: Application
    Filed: November 21, 2012
    Publication date: October 2, 2014
    Applicant: The General Hospital Corporation
    Inventors: Ralph Weissleder, Hakho Lee, David Issadore
  • Plectin-1 targeted agents for detection and treatment of pancreatic ductal adenocarcinoma
    Patent number: 8829159
    Abstract: Described herein are compositions and methods for cancer cell biomarkers, such as pancreatic ductal adenocarcinoma (PDAC) cell biomarkers, and binding molecules for diagnosis and treatment of cancer, e.g., PDAC. Methods of identifying “accessible” proteomes are disclosed for identifying cancer biomarkers, such as plectin-1, a PDAC biomarker. Additionally, imaging compositions are provided comprising magnetofluorescent nanoparticles conjugated to peptide ligands for identifying PDACs.
    Type: Grant
    Filed: April 14, 2009
    Date of Patent: September 9, 2014
    Assignee: The General Hospital Corporation
    Inventors: Kimberly Kelly, Ralph Weissleder, Nabeel Bardeesy
  • Molecular Analysis of Tumor Samples
    Publication number: 20140178901
    Abstract: This invention relates to methods for diagnosing cancer, e.g., cancer of epithelial origin, by detecting the presence of tumor cells in a sample, based (at least in some embodiments) on the quantification of levels of four biomarkers, MUC1, EGFR, EpCAM, and HER2. In some embodiments, the methods are performed using diagnostic magnetic resonance (DMR), e.g., with a portable relaxometer or MR imager.
    Type: Application
    Filed: March 21, 2012
    Publication date: June 26, 2014
    Applicant: The General Hospital Corporation
    Inventors: Ralph Weissleder, Hakho Lee, Cesar Castro
  • FACTOR XII INHIBITORS FOR THE TREATMENT OF SILENT BRAIN ISCHEMIA AND ISCHEMIA OF OTHER ORGANS
    Publication number: 20140072572
    Abstract: Silent brain ischemia (SBI) or ischemia of other organs can result from an embolism that is introduced into the arterial system during a medical procedure. The application provides a method of administering a FXII inhibitor in a patient receiving a medical procedure and animal models useful for studying ischemia including SBI and ischemia in other organs, and for evaluating candidate therapeutics.
    Type: Application
    Filed: March 9, 2012
    Publication date: March 13, 2014
    Inventors: Matthias Nahrendorf, Ralph Weissleder, Gerhard Dickneite, Guido Stoll, Marc Nolte
  • Imaging Beta Cell Mass
    Publication number: 20140056812
    Abstract: The present disclosure provides compositions and methods based on peptide-detectable agent conjugates that are useful for imaging beta cell mass.
    Type: Application
    Filed: June 27, 2011
    Publication date: February 27, 2014
    Applicant: THE GENERAL HOSPITAL CORPORATION
    Inventors: Ralph Weissleder, Thomas Reiner, Edmund J. Keliher
  • WATER RELAXATION-BASED SENSORS
    Publication number: 20140017816
    Abstract: This invention relates to magnetic resonance-based sensors and related methods.
    Type: Application
    Filed: September 13, 2013
    Publication date: January 16, 2014
    Applicant: The General Hospital Corporation
    Inventors: Lee Josephson, Yi Sun, Ralph Weissleder
  • Detection of Targets Using Magnetic Resonance
    Publication number: 20140011217
    Abstract: A portable magnetic resonance system includes a permanent magnet, a nuclear magnetic resonance probe, and control electronics. The control electronics are configured to transmit to the probe a magnetic resonance excitation signal having an excitation frequency f, receive from the probe a magnetic resonance measurement signal, detect in the magnetic resonance measurement signal a magnetic resonance frequency f0, and automatically adjust the excitation frequency f until the difference between the excitation frequency and the magnetic resonance frequency is approximately equal to a target offset.
    Type: Application
    Filed: March 21, 2012
    Publication date: January 9, 2014
    Applicant: THE GENERAL HOSPITAL CORPORATION
    Inventors: Ralph Weissleder, Hakho Lee, David Issadore
  • Compositions And Methods For In Vivo Imaging
    Publication number: 20130309170
    Abstract: This document relates to compounds useful for targeting PARP1. Also provided herein are methods for using such compounds to detect and image cancer cells.
    Type: Application
    Filed: November 22, 2011
    Publication date: November 21, 2013
    Applicant: THE GENERAL HOSPITAL CORPORATION
    Inventors: Thomas Reiner, Edmund J. Keliher, Ralph Weissleder
  • Magnetic-nanoparticle conjugates and methods of use
    Patent number: 8569078
    Abstract: The present invention provides novel compositions of binding moiety-nanoparticle conjugates, aggregates of these conjugates, and novel methods of using these conjugates, and aggregates. The nanoparticles in these conjugates can be magnetic metal oxides, either monodisperse or polydisperse. Binding moieties can be, e.g., oligonucleotides, polypeptides, or polysaccharides. Oligonucleotide sequences are linked to either non-polymer surface functionalized metal oxides or with functionalized polymers associated with the metal oxides. The novel compositions can be used in assays for detecting target molecules, such as nucleic acids and proteins, in vitro or as magnetic resonance (MR) contrast agents to detect target molecules in living organisms.
    Type: Grant
    Filed: May 3, 2010
    Date of Patent: October 29, 2013
    Assignee: The General Hospital Corporation
    Inventors: Lee Josephson, Ralph Weissleder, J. Manuel Perez
  • Self-Assembled Magnetic Arrays
    Publication number: 20130271250
    Abstract: A magnetic device includes a substrate and an array including a plurality of magnetic particles, in which a magnetic dipole moment direction of each magnetic particle in a majority of the magnetic particles in the array alternates with respect to a magnetic dipole direction of a directly adjacent magnetic particle or directly adjacent magnetic particles in the array.
    Type: Application
    Filed: September 27, 2011
    Publication date: October 17, 2013
    Applicant: THE GENERAL HOSPITAL CORPORATION
    Inventors: Ralph Weissleder, Hakho Lee, David Issadore
  • Water relaxation-based sensors
    Patent number: 8535949
    Abstract: This invention relates to magnetic resonance-based sensors and related methods.
    Type: Grant
    Filed: November 4, 2009
    Date of Patent: September 17, 2013
    Assignee: The General Hospital Corporation
    Inventors: Lee Josephson, Yi Sun, Ralph Weissleder