Abstract: A micro flow device for separating or isolating cells from a bodily fluid or other liquid sample uses a flow path where straight-line flow is interrupted by a pattern of transverse posts. The posts are spaced across the width of an expanded collection chamber region in the flow path, extending between the upper and lower surfaces thereof; they have rectilinear surfaces, being curved in cross-sections, e.g. circular or tear-drop shaped, and are randomly arranged so as to disrupt streamlined flow. The device is oriented so that its lower surface is aligned at about 45° to the horizontal. Sequestering agents, such as Abs, which are attached to surfaces of the collection region via a hydrophilic coating, preferably a permeable hydrogel containing isocyanate moieties, are highly effective in capturing cells or other targeted biomolecules while the remainder of the liquid sample exits horizontally.

Abstract: A micro flow device (11, 71) for separating or isolating cells from a bodily fluid or other liquid sample uses a flow path where straight-line flow is interrupted by a pattern of transverse posts (23, 81). The posts are spaced across the width of an expanded collection chamber region (17, 75) in the flow path, extending between the upper and lower surfaces thereof; they have rectilinear surfaces, being curved in cross-sections, e.g. circular or tear-drop shaped, and are randomly arranged so as to disrupt streamlined flow. The device is oriented so that its lower surface is aligned at about 45° to the horizontal. Sequestering agents, such as Abs, which are attached to surfaces of the collection region via a hydrophilic coating, preferably a permeable hydrogel containing isocyanate moieties, are highly effective in capturing cells or other targeted biomolecules while the remainder of the liquid sample exits horizontally.

Abstract: Embodiments of the present disclosure disclose a method and a computing unit for imaging of wider angle fundus of an eye of a subject. The method discloses guiding placement of an imaging device at set of predetermined locations on the eye. The method further comprises receiving set of images of the fundus of the eye upon determining the imaging device to be at least one of the set of predetermined locations, wherein the set of images are captured by the imaging device upon receiving instructions from the computing unit. The method further comprises determining quality of the set of images and concatenating the one or more images to provide a wider angle target image of the fundus of the eye.

Abstract: The present disclosure relates to an apparatus for multi-mode imaging of an eye. The apparatus integrates at least two modes namely OCT, hyper-/multi-spectral imaging, and RGB/IR. The apparatus captures high resolution and larger dynamic range OCT and hyper-/multi-spectral images, in a high dynamic range multi-wavelength image data. The apparatus captures the images through an optical path sharing one or more optical elements and a detection unit. The captured images are processed by a computing device associated with the apparatus for either OCT or hyper-spectral imaging.

Abstract: The present disclosure provides a portable haemodialysis system, comprising at least one haemodialyser configured to purify a biological sample. At least one fuel cell is fluidly connected to the at least one haemodialyser, the at least one fuel cell is adapted to receive oxygen and hydrogen from at least one oxygen and hydrogen storage units to generate energy and water. At least one energy reservoir is connected to the at least one fuel cell which is configured to store the energy generated in the at least one fuel cell. The water generated in the at least one fuel cell is supplied to the at least one haemodialyser for purification of the biological sample, and the energy stored in the at least one energy reservoir is used to power the haemodialyser during operation.

Abstract: A micro flow device (11, 71) for separating or isolating cells from a bodily fluid or other liquid sample uses a flow path where straight-line flow is interrupted by a pattern of transverse posts (23, 81). The posts are spaced across the width of an expanded collection chamber region (17, 75) in the flow path, extending between the upper and lower surfaces thereof; they have rectilinear surfaces, being curved in cross-sections, e.g. circular or tear-drop shaped, and are randomly arranged so as to disrupt streamlined flow. The device is oriented so that its lower surface is aligned at about 45° to the horizontal. Sequestering agents, such as Abs, which are attached to surfaces of the collection region via a hydrophilic coating, preferably a permeable hydrogel containing isocyanate moieties, are highly effective in capturing cells or other targeted biomolecules while the remainder of the liquid sample exits horizontally.

Abstract: A micro flow device (11, 71) for separating or isolating cells from a bodily fluid or other liquid sample uses a flow path where straight-line flow is interrupted by a pattern of transverse posts (23, 81). The posts are spaced across the width of an expanded collection chamber region (17, 75) in the flow path, extending between the upper and lower surfaces thereof; they have rectilinear surfaces, being curved in cross-sections, e.g. circular or tear-drop shaped, and are randomly arranged so as to disrupt streamlined flow. The device is oriented so that its lower surface is aligned at about 45° to the horizontal. Sequestering agents, such as Abs, which are attached to surfaces of the collection region via a hydrophilic coating, preferably a permeable hydrogel containing isocyanate moieties, are highly effective in capturing cells or other targeted biomolecules while the remainder of the liquid sample exits horizontally.

Abstract: Non-tactile and non-evasive tonometer utilizing air flow with a definite amount of pressure to the eye and a mechanism to deflate the thin foil set that is placed near to eye ball such that re-bounded air hits on it. The mechanism involves acquiring or capturing then the images of the known pattern marking on thin foils both before and after deflating process due to rebounded air. On evaluating the deformation of the pattern appearing in the images obtained before and after air flow and calibrating the deformation with respect to size, translation, rotation and scaling parameters due the different pressure level that hits the eye ball and that rebounds on to thin foils, we arrive at a scheme of measuring the intraocular pressure of human eye. This intraocular pressure is used as a parameter for the ophthalmologist to diagnose glaucoma impairment of human beings.

Abstract: The present disclosure relates to method and apparatus for image recovery and image correction. In an embodiment, the present disclosure relates to recovering images that are occluded due to some material in the optical path while capturing the images. The detection of noisy region is performed by taking the residual image between the images yielded by large sized and small sized Gaussian window smoothening filters on red, green and blue channels. The recovery of the image that alleviates the noise is obtained by point-wise multiplication of the above said residue image with a suitably synthesized Gaussian distributed co-efficient of same size.

Abstract: The present disclosure relates to image filtering techniques for enhancing image quality. In one embodiment, two filtering techniques are applied on an image. Firstly, an adaptive weighted median filtering operation is performed on an acquired low contrast image corrupted by impulsive noise. Subsequently, a guided image filtering on the image obtained from adaptive weighted median filtering operation to de-blur and enhance the contrast that ultimately assures to preserve the edges of the images. In addition, the image filtering for enhancing image quality is enhanced by several variations of data adaptive guided image filtering and adaptive window sizes for guided image filtering techniques.

Abstract: The present disclosure relates to image filtering techniques for enhancing image quality. In one embodiment, two filtering techniques are applied on an image. Firstly, an adaptive weighted median filtering operation is performed on an acquired low contrast image corrupted by impulsive noise. Subsequently, a guided image filtering on the image obtained from adaptive weighted median filtering operation to de-blur and enhance the contrast that ultimately assures to preserve the edges of the images. In addition, the image filtering for enhancing image quality is enhanced by several variations of data adaptive guided image filtering and adaptive window sizes for guided image filtering techniques.

Abstract: The present disclosure relates to method and apparatus for image recovery and image correction. In an embodiment, the present disclosure relates to recovering images that are occluded due to some material in the optical path while capturing the images. The detection of noisy region is performed by taking the residual image between the images yielded by large sized and small sized Gaussian window smoothening filters on red, green and blue channels. The recovery of the image that alleviates the noise is obtained by point-wise multiplication of the above said residue image with a suitably synthesized Gaussian distributed co-efficient of same size.

Abstract: The present invention provides probes, kits and methods for specifically binding mRNA of target cells, e.g., fetal cells, that allows visualization of the target cells, under conditions that allows a second probe to specifically bind the target cell's chromosomal DNA.

Abstract: The present invention discloses methods of determining the RhD genotype of subject. In particular, the invention provides a non-invasive method of determining fetal RhD genotype from a maternal biological sample containing fetal cells. The invention also provides novel probes and primers useful in the described methods. Kits and mixtures comprising the novel probes and primers are also disclosed.

Abstract: The present invention provides methods for isolating cell free nucleic acid, e.g., apoptotic or fetal nucleic acids and methods of detecting neoplastic cells or identifying the genetic composition of a fetus. The invention also provides magnetic particles comprising an anti-DNA antibody, and kits comprising the magnetic particles.

Abstract: The present invention provides compositions comprising a solution of a compound useful for lysing biological cells and methods for using the compositions to lyse biological cells and to isolate nucleic acid.

Abstract: The present invention provides methods and kits useful for genetic testing or screening of fetuses using nucleic acid samples isolated from cervical mucus samples of fetus hosts.

Abstract: The present invention provides novel fluorescent compounds and covalent attachment chemistries which facilitate the use of these compounds as labels for ultrasensitive and quantitative fluorescent detection of low levels of biomolecules. In a preferred embodiment, the fluorescent labels of this invention are novel derivatives of the hydroxy-pyrene trisulphonic and disulphonic acids which may be used in any assay in which radioisotopes, colored dyes or other fluorescent molecules are currently used. Thus, for example, any assay using labeled antibodies, proteins, oligonucleotides or lipids, including fluorescent cell sorting, fluorescence microscopy (including dark-field microscopy), fluorescence polarization assays, ligand, receptor binding assays, receptor activation assays and diagnostic assays can benefit from use of the compounds disclosed herein.

Abstract: The present invention provides novel fluorescent compounds and covalent attachment chemistries which facilitate the use of these compounds as labels for ultrasensitive and quantitative fluorescent detection of low levels of biomolecules. In a preferred embodiment, the fluorescent labels of this invention are novel derivatives of the hydroxy-pyrene trisulpbonic and disulphonic acids which may be used in any assay in which radioisotopes, colored dyes or other fluorescent molecules are currently used. Thus, for example, any assay using labeled antibodies, proteins, oligonucleotides or lipids, including fluorescent cell sorting, fluorescence microscopy (including dark-field microscopy), fluorescence polarization assays, ligand, receptor binding assays, receptor activation assays and diagnostic assays can benefit from use of the compounds disclosed herein.

Abstract: The present invention provides methods and kits useful for genetic testing or screening of fetuses using nucleic acid samples isolated from cervical mucus samples of fetus hosts.