Patents by Inventor Ramachandra N. Rao

Ramachandra N. Rao has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Platenolide synthase gene
    Patent number: 5945320
    Abstract: A DNA molecule isolated from Streptomyces ambofaciens encodes the multi-functional proteins which direct the synthesis of the polyketide platenolide.
    Type: Grant
    Filed: February 21, 1997
    Date of Patent: August 31, 1999
    Assignee: Eli Lilly and Company
    Inventors: Stanley G. Burgett, Stuart A. Kuhstoss, Ramachandra N. Rao, Mark A. Richardson, Paul R. Rosteck, Jr.
  • Fusion proteins comprising cell cycle regulatory proteins
    Patent number: 5814503
    Abstract: The present invention provides novel fusion proteins comprising cyclins and CDKs. A preferred embodiment of the invention provides fusion proteins comprising human cyclin D1 and human CDK4. The fusion proteins of the invention optionally contain modifications, which facilitate their purification. Addition of histidine residues to selected constructs allows purification via immobilized metal affinity chromatography. Antigenic determinants allowing monoclonal antibody-based affinity chromatography purification are provided in selected embodiments of the invention. Protease cleavage sites are incorporated in selected constructs to allow cleavage of the regions incorporated in the cyclin-CDK fusion proteins for purification. Additional modifications which facilitate purification include strepavadin binding domains and antigenic determinants for antibody affinity chromatography.
    Type: Grant
    Filed: December 19, 1996
    Date of Patent: September 29, 1998
    Assignee: Eli Lilly and Company
    Inventors: Steven Kovacevic, Keith A. Otto, Ramachandra N. Rao
  • Activator gene for macrolide biosynthesis
    Patent number: 5514544
    Abstract: A gene encoding activator protein (srmR) for increasing transcriptional efficiency of macrolide biosynthetic genes is disclosed and claimed. Methods for using srmR to increase macrolide biosynthetic gene transcription and identifying further macrolide biosynthetic pathways are disclosed. Recombinant DNA vectors comprising the srmR gene are disclosed.
    Type: Grant
    Filed: July 26, 1991
    Date of Patent: May 7, 1996
    Assignee: Eli Lilly and Company
    Inventors: Ramachandra N. Rao, Jan R. Turner
  • Recombinant DNA cloning vectors and the eukaryotic and prokaryotic transformants thereof
    Patent number: 4727028
    Abstract: The present invention comprises novel recombinant DNA cloning and expression vectors which confer hygromycin B and/or G418 resistance to eukaryotic and prokaryotic host cells. The novel recombinant DNA vectors are derived from plasmid pKC203, a plasmid which can be isolated from E. coli JR225 (ATCC 31912). The hygromycin B and G418 resistance-conferring genes can be isolated on the 7.5 kb BglII restriction fragment or the 2.5 kb SalI-BglII restriction fragment of plasmid pKC203. The eukaryotic recombinant DNA vectors of the present invention are prepared by inserting such resistance-conferring restriction fragments into a vector, such as plasmid pSV5gpt, that comprises a eukaryotic promoter and the necessary functions for maintenance of the vector as an extrachromosomal element or as an integrated sequence in the host cell chromosomal DNA. Furthermore, the present invention comprises useful derivatives of plasmid pKC203 which, although comprising no eukaryotic elements, are useful recombinant vectors for E.
    Type: Grant
    Filed: September 30, 1983
    Date of Patent: February 23, 1988
    Assignee: Eli Lilly and Company
    Inventors: Robert F. Santerre, Ramachandra N. Rao