Patents by Inventor Ramakrishna Seethala

Ramakrishna Seethala has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • FLUORESCENCE POLARIZATION BINDING ASSAY FOR CHARACTERIZING GLUCOKINASE LIGANDS
    Publication number: 20100311072
    Abstract: The subject matter disclosed and claimed herein concerns measuring the binding affinity of glucokinase (“GK”) using a fluorescence polarization (“FP”) assay. The FP method includes use of modified GK ligands bound to a fluorescent label. Binding affinity is determined by measuring displacement of fluorescent ligand by the known or suspected GK ligands. The subject matter disclosed and claimed herein provides a robust high-throughput FP assay for the determination of binding affinity of ligands to glucokinase. The FP binding assay displayed both glucose and nucleotide dependence, and a useful dynamic range. The binding IC50 data correlated well with GK activation EC50 data.
    Type: Application
    Filed: January 28, 2009
    Publication date: December 9, 2010
    Inventors: Ramakrishna Seethala, Jessica Leihwa Dong, Kevin M. O'Malley
  • Scintillation proximity assay method of measuring acetyl CoA carboxylase enzyme activity
    Patent number: 7384757
    Abstract: The present invention relates generally to enzyme assays and more particularly to a rapid, sensitive, reliable and robust homogeneous assay for acetyl CoA carboxylase activity comprising a coupled enzyme assay in a scintillation proximity format suitable for high-throughput screening. In one aspect, the assay couples ACC and FAS and the product, palmitic acid is then detected by scintillation counting. The invention also relates to the identification of modulators of ACC activity.
    Type: Grant
    Filed: March 30, 2005
    Date of Patent: June 10, 2008
    Assignee: Bristol-Myers Squibb Company
    Inventors: Ramakrishna Seethala, Dong Cheng
  • GLUCOKINASE ACTIVITY ASSAYS FOR MEASURING KINETIC AND ACTIVATION PARAMETERS
    Publication number: 20080085528
    Abstract: The subject matter disclosed and claimed herein relates to novel in vitro assays for measuring glucokinase activity and use of these assays for identifying modulators of glucokinase.
    Type: Application
    Filed: October 10, 2007
    Publication date: April 10, 2008
    Inventors: Jovita Marcinkeviciene, Kevin O'Malley, Mark Kirby, Aiying Wang, Lisa Kopcho, Ramakrishna Seethala
  • Scintillation proximity assay method of measuring acetyl CoA carboxylase enzyme activity
    Publication number: 20050221410
    Abstract: The present invention relates generally to enzyme assays and more particularly to a rapid, sensitive, reliable and robust homogeneous assay for acetyl CoA carboxylase activity comprising a coupled enzyme assay in a scintillation proximity format suitable for high-throughput screening. In one aspect, the assay couples ACC and FAS and the product, palmitic acid is then detected by scintillation counting. The invention also relates to the identification of modulators of ACC activity.
    Type: Application
    Filed: March 30, 2005
    Publication date: October 6, 2005
    Inventors: Ramakrishna Seethala, Dong Cheng
  • Dihydropyridine npy antagonists: piperazine derivatives
    Patent number: 5635503
    Abstract: A series of non-peptidergic antagonists of NPY have been synthesized and are comprised of piperazine and homopiperazine derivatives of 4-phenyl-1,4-dihydropyridines of Formula (I). ##STR1## As antagonists of NPY-induced feeding behavior, these compounds are expected to act as effective anorexiant agents in promoting weight loss and treating eating disorders.
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: June 3, 1997
    Assignee: Bristol-Myers Squibb Company
    Inventors: Graham S. Poindexter, Marc Bruce, Graham Johnson, Michael Kozlowski, Karen LeBoulluec, Ivo Monkovic, Ramakrishna Seethala, Charles P. Sloan