Abstract: Disclosed herein is a method of operating a microfluidic system, a microfluidic system for manipulating sample objects, and a control system for operating a microfluidic system. The method according to the invention is for operating a microfluidic system that comprises a manipulation zone for manipulating sample objects flowing through the manipulation zone, a first detection zone arranged upstream of or in the manipulation zone and a second detection zone arranged upstream of, in or downstream of the manipulation zone. A first count is determined that characterizes a number of sample objects flowing through the first detection zone. Sample objects are manipulated in the manipulation zone. A second count is determined that characterizes a number of sample objects flowing through the second detection zone. One or more manipulation parameters for manipulating the sample objects in the manipulation zone are adjusted based on the first count and the second count.

Abstract: The present disclosure teaches an apparatus and a method for providing one or more substance liquids to a microfluidic channel network. The microfluidic apparatus includes valves for switching the one or more substance liquids to a microfluidic channel network. The apparatus can be used to generate a sequence of the one or more substance liquids as individual droplets in an immiscible separation liquid wherein individual ones of the sequence of droplets are located between the separation liquid.

Abstract: The present invention relates to the field of microfluidics and in particular to methods for co-localizing a particle comprising DNA and/or RNA with a known barcode oligonucleotide. Thereby, the transcriptome or DNA of a cell can be barcoded and correlated to a cell phenotype or examined for the effect of a drug on the cell. The invention also provides microfluidic devices and systems having properties which make them particularly suitable for use in the methods of the invention.

Abstract: A sorting device (10) is disclosed comprising a channel (40) adapted to allow passage of a plurality of samples in a fluid to a first side of a sorting junction (50) and a plurality of Braille valves (105) connected by a plurality of connectors to the sorting junction (50). An analysis device (80), such as a camera, is used for analysing ones of the plurality of the samples and adapted to control ones of the plurality of Braille valves depending on properties of the analysed ones of the plurality of the samples.

Abstract: The present disclosure teaches an apparatus and a method for providing one or more substance liquids to a microfluidic channel network. The microfluidic apparatus includes valves for switching the one or more substance liquids to a microfluidic channel network. The apparatus can be used to generate a sequence of the one or more substance liquids as individual droplets in an immiscible separation liquid wherein individual ones of the sequence of droplets are located between the separation liquid.

Abstract: An apparatus and a method for providing one or more substance liquids to a microfluidic channel network (30). The microfluidic apparatus includes valves for switching the one or more substance liquids to a microfluidic channel network (30). The apparatus can be used to generate a sequence of the one or more substance liquids as individual droplets in an immiscible separation liquid wherein individual ones of the sequence of droplets are located between the separation liquid.

Abstract: The present invention relates to the field of microfluidics and in particular to methods for co-localizing a particle comprising DNA and/or RNA with a known barcode oligonucleotide. Thereby, the transcriptome or DNA of a cell can be barcoded and correlated to a cell phenotype or examined for the effect of a drug on the cell. The invention also provides microfluidic devices and systems having properties which make them particularly suitable for use in the methods of the invention.

Abstract: A sorting device (10) is disclosed comprising a channel (40) adapted to allow passage of a plurality of samples in a fluid to a first side of a sorting junction (50) and a plurality of Braille valves (105) connected by a plurality of connectors to the sorting junction (50). An analysis device (80), such as a camera, is used for analysing ones of the plurality of the samples and adapted to control ones of the plurality of Braille valves depending on properties of the analysed ones of the plurality of the samples.

Abstract: An apparatus and a method for providing one or more substance liquids to a microfluidic channel network (30). The microfluidic apparatus includes valves for switching the one or more substance liquids to a microfluidic channel network (30). The apparatus can be used to generate a sequence of the one or more substance liquids as individual droplets in an immiscible separation liquid wherein individual ones of the sequence of droplets are located between the separation liquid.

Abstract: The present disclosure teaches an apparatus and a method for providing one or more substance liquids to a microfluidic channel network (30). The microfluidic apparatus includes valves for switching the one or more substance liquids to a microfluidic channel network (30). The apparatus can be used to generate a sequence of the one or more substance liquids as individual droplets in an immiscible separation liquid wherein individual ones of the sequence of droplets are located between the separation liquid.

Abstract: The present disclosure relates to a microfluidic device and a method allowing the generating and screening of combinatorial samples. A microfluidic device for producing droplets of at least one sample into an immiscible phase is provided, the device comprising a droplet maker connecting an immiscible phase channel and a sample channel having at least one sample inlet connected to at least one sample inlet channel injecting the at least one sample into the sample channel, wherein the injection of the at least one sample is controlled by at least one sample valve, so that the at least one sample flows either towards a sample waste outlet or into the at least one sample inlet channel, wherein different sample inlet channel of the at least one sample inlet channel have the same hydrodynamic resistance resulting from the length, height and width of each sample inlet channel upstream of the droplet maker.

Abstract: The present disclosure teaches an apparatus and a method for providing one or more substance liquids to a microfluidic channel network (30). The microfluidic apparatus includes valves for switching the one or more substance liquids to a microfluidic channel network (30). The apparatus can be used to generate a sequence of the one or more substance liquids as individual droplets in an immiscible separation liquid wherein individual ones of the sequence of droplets are located between the separation liquid.