Patents by Inventor Ramnath Sasisekharan

Ramnath Sasisekharan has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method for obtaining a modified heparinase gene
    Patent number: 5830726
    Abstract: The cloning of the heparinase gene from Flavobacterium Heparinum using the polymerase chain reaction is described. The Open Reading Frame (ORF) corresponded to 1152 base pairs encoding a precursor protein of MW 43,800 daltons. The amino acid sequence reveals a 20-residue leader peptide. The gene was expressed in two expression systems in E. coli.
    Type: Grant
    Filed: May 19, 1995
    Date of Patent: November 3, 1998
    Assignee: Massachusetts Institute of Technology
    Inventors: Ramnath Sasisekharan, Kelley Moremen, Charles I. Cooney, Joseph J. Zimmermann, Robert S. Langer
  • Heparinase gene from flavobacterium heparinum
    Patent number: 5714376
    Abstract: The cloning of the heparinase gene from Flavobacterium Heparinum using the polymerase chain reaction is described. The Open Reading Frame (ORF) corresponded to 1152 base pairs encoding a precursor protein of MW 43,800 daltons. The amino acid sequence reveals a 20-residue leader peptide. The gene was expressed in two expression systems in E. coli.
    Type: Grant
    Filed: October 23, 1991
    Date of Patent: February 3, 1998
    Assignee: Massachusetts Institute of Technology
    Inventors: Ramnath Sasisekharan, Kelley Moremen, Charles L. Cooney, Joseph J. Zimmermann, Robert S. Langer
  • Purification, composition and specificity of heparinase I, II, and III from flavobacterium heparinum
    Patent number: 5569600
    Abstract: A single, reproducible scheme to simultaneously purify all three of the heparin lyases from F. heparinum to apparent homogeneity is disclosed herein. The kinetic properties of the heparin lyases have been determined as well as the conditions to optimize their activity and stability. Monoclonal antibodies to the three heparinases are also described and are useful for detection, isolation and characterization of the heparinases.
    Type: Grant
    Filed: January 26, 1995
    Date of Patent: October 29, 1996
    Assignees: Massachusetts Institute of Technology, University of Iowa Research Foundation
    Inventors: Ramnath Sasisekharan, Daniel L. Lohse, Charles L. Cooney, Robert J. Linhardt, Robert S. Langer
  • Method for inhibiting angiogenesis using heparinase
    Patent number: 5567417
    Abstract: Pharmaceutical compositions for delivering an effective dose to a desired site of a heparinase. These compositions are based on the discovery that heparinase alone can inhibit angiogenesis. The effective dosage is dependent not only on the heparinase, but also on the method and means of delivery, which can be localized or systemic. For example, in some applications, as in the treatment of psoriasis or diabetic retinopathy, the inhibitor is delivered in a topical ophthalmic carrier. In other applications, as in the treatment of solid tumors, the inhibitor is delivered by means of a biodegradable, polymeric implant.
    Type: Grant
    Filed: May 1, 1995
    Date of Patent: October 22, 1996
    Assignees: Massachusetts Institute of Technology, Children's Medical Center Corporation
    Inventors: Ramnath Sasisekharan, Marsha A. Moses, Matthew A. Nugent, Charles L. Cooney, Robert S. Langer
  • Purification of heparinase I, II, and III from Flavobacterium heparinum
    Patent number: 5389539
    Abstract: A purified heparinase I, II and III free of lyase activity and each having a molecular weight of 42,800 84,100, 70,800, respectively, are produced by culturing Flavobacterium heparinum. The kinetic properties of the heparinases have been determined as well as the conditions to optimize their activity and stability.
    Type: Grant
    Filed: November 30, 1992
    Date of Patent: February 14, 1995
    Assignees: Massachusetts Institute of Technology, University of Iowa Research Foundation
    Inventors: Ramnath Sasisekharan, Daniel L. Lohse, Charles L. Cooney, Robert J. Linhardt, Robert S. Langer
  • Purification of Heparinase I, II and III from Flavobacterium heparinum
    Patent number: RE41328
    Abstract: A purified heparinase I, II and III free of lyase activity and each having a molecular weight of 42,800 84,100, 70,800, respectively, are produced by culturing Flavobacterium heparinum. The kinetic properties of the heparinases have been determined as well as the conditions to optimize their activity and stability.
    Type: Grant
    Filed: August 27, 2008
    Date of Patent: May 11, 2010
    Assignees: Massachusetts Institute of Technology, University of Iowa Research Foundation
    Inventors: Ramnath Sasisekharan, Charles L. Cooney, Robert S. Langer, Daniel L. Lohse, Robert J. Linhardt
  • Purification, composition and specificity of Heparinase I, II, and III from Flavobacterium heparinum
    Patent number: RE41461
    Abstract: A single, reproducible scheme to simultaneously purify all three of the heparin lyases from F. heparinum to apparent homogeneity is disclosed herein. The kinetic properties of the heparin lyases have been determined as well as the conditions to optimize their activity and stability. Mono-clonal antibodies to the three heparinases are also described and are useful for detection, isolation and characterization of the heparinases.
    Type: Grant
    Filed: August 27, 2008
    Date of Patent: July 27, 2010
    Assignees: Massachusetts Institute of Technology, University of Iowa Research Foundation
    Inventors: Daniel L. Lohse, Robert J. Linhardt, Ramnath Sasisekharan, Charles L. Cooney, Robert S. Langer