Patents by Inventor Ramon Gonzalez
Ramon Gonzalez has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11913049Abstract: An engineered microorganism(s) with novel pathways for the conversion of short-chain hydrocarbons to fuels and chemicals (e.g. carboxylic acids, alcohols, hydrocarbons, and their alpha-, beta-, and omega-functionalized derivatives) is described. Key to this approach is the use of hydrocarbon activation enzymes able to overcome the high stability and low reactivity of hydrocarbon compounds through the cleavage of an inert C—H bond. Oxygen-dependent or oxygen-independent activation enzymes can be exploited for this purpose, which when combined with appropriate pathways for the conversion of activated hydrocarbons to key metabolic intermediates, enables the generation of product precursors that can subsequently be converted to desired compounds through established pathways. These novel engineered microorganism(s) provide a route for the production of fuels and chemicals from short chain hydrocarbons such as methane, ethane, propane, butane, and pentane.Type: GrantFiled: May 19, 2020Date of Patent: February 27, 2024Inventors: Ramon Gonzalez, James Clomburg, Alexander Chou
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Publication number: 20230383319Abstract: The use of microorganisms to make alpha-functionalized chemicals and fuels, (e.g. alpha-functionalized carboxylic acids, alcohols, hydrocarbons, amines, and their beta-, and omega-functionalized derivatives), by utilizing an iterative carbon chain elongation pathway that uses functionalized extender units. The core enzymes in the pathway include thiolase, dehydrogenase, dehydratase and reductase. Native or engineered thiolases catalyze the condensation of either unsubstituted or functionalized acyl-CoA primers with an alpha-functionalized acetyl-CoA as the extender unit to generate alpha-functionalized ?-keto acyl-CoA. Dehydrogenase converts alpha-functionalized ?-keto acyl-CoA to alpha-functionalized ?-hydroxy acyl-CoA. Dehydratase converts alpha-functionalized ?-hydroxy acyl-CoA to alpha-functionalized enoyl-CoA. Reductase converts alpha-functionalized enoyl-CoA to alpha-functionalized acyl-CoA. The platform can be operated in an iterative manner (i.e.Type: ApplicationFiled: June 1, 2023Publication date: November 30, 2023Inventor: Ramon GONZALEZ
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Publication number: 20230332191Abstract: Many biotechnologically relevant organisms cannot utilize cheap and abundant one carbon feedstocks, e.g. CO2, CO, formaldehyde, methanol, and methane, for growth and instead prefer complex feedstocks such as sugars. Disclosed herein is a system that enables organisms to consume one carbon molecules for growth and maintenance via a formyl-CoA elongation pathway. Utilization of one carbon feedstocks can replace the use of sugar as the primary means of cultivating organisms in biotechnological applications. This has the potential to be more cost effective and avoid the controversial use of food as feedstocks. Intermediates of the formyl-CoA elongation pathway may be also be converted to desired chemical products.Type: ApplicationFiled: August 26, 2021Publication date: October 19, 2023Inventors: Ramon GONZALEZ, Alexander CHOU, James CLOMBURG, Fayin ZHU, Seung Hwan LEE
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Patent number: 11781120Abstract: This disclosure generally relates to the use of microorganisms to make various functionalized polyketides through polyketoacyl-CoA thiolase-catalyzed non-decarboxylative condensation reactions instead of decarboxylative reactions catalyzed by polyketide synthases. Native or engineered polyketoacyl-CoA thiolases catalyze the non-decarboxylative Claisen condensation in an iterative manner (i.e. multiple rounds) between two either unsubstituted or functionalized ketoacyl-CoAs (and polyketoacyl-CoAs) serving as the primers and acyl-CoAs serving as the extender unit to generate (and elongate) polyketoacyl-CoAs. Before the next round of polyketoacyl-CoA thiolase reaction, the ?-keto group of the polyketide chain of polyketoacyl-CoA can be reduced and modified step-wise by 3-OH-polyketoacyl-CoA dehydrogenase or polyketoenoyl-CoA hydratase or polyketoenoyl-CoA reductase. Dehydrogenase converts the ?-keto group to ?-hydroxy group. Hydratase converts the ?-hydroxy group to ?-?-double-bond.Type: GrantFiled: July 15, 2020Date of Patent: October 10, 2023Inventor: Ramon Gonzalez
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Patent number: 11697830Abstract: The use of microorganisms to make alpha-functionalized chemicals and fuels, (e.g. alpha-functionalized carboxylic acids, alcohols, hydrocarbons, amines, and their beta-, and omega-functionalized derivatives), by utilizing an iterative carbon chain elongation pathway that uses functionalized extender units. The core enzymes in the pathway include thiolase, dehydrogenase, dehydratase and reductase. Native or engineered thiolases catalyze the condensation of either unsubstituted or functionalized acyl-CoA primers with an alpha-functionalized acetyl-CoA as the extender unit to generate alpha-functionalized ?-keto acyl-CoA. Dehydrogenase converts alpha-functionalized ?-keto acyl-CoA to alpha-functionalized ?-hydroxy acyl-CoA. Dehydratase converts alpha-functionalized ?-hydroxy acyl-CoA to alpha-functionalized enoyl-CoA. Reductase converts alpha-functionalized enoyl-CoA to alpha-functionalized acyl-CoA. The platform can be operated in an iterative manner (i.e.Type: GrantFiled: March 13, 2020Date of Patent: July 11, 2023Inventors: Ramon Gonzalez, James M. Clomburg, Seokjung Cheong
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Publication number: 20220251613Abstract: Methods of using microorganisms to make chemicals and fuels, including carboxylic acids, alcohols, hydrocarbons, and their alpha-, beta-, and omega-functionalized derivatives are described. Native or engineered thiolases are used condense a growing acyl-ACP and acetyl-ACP in combination with type II fatty acid synthesis. The resulting fatty acid biosynthesis cycle has an ATP yield analogous to the functional reverse ?-oxidation cycle.Type: ApplicationFiled: April 5, 2022Publication date: August 11, 2022Inventors: Ramon GONZALEZ, James M. CLOMBURG
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Publication number: 20220162661Abstract: The production of substituted 2-hydroxyacyl-CoA molecules by a novel reaction is described. The reaction involves the condensation of formyl-CoA with a carbonyl-containing molecule. Such carbonyl-containing molecules include a substituted aldehyde and a ketone. The reaction is catalyzed by enzymes using a TPP-dependent mechanism. Also described is the production of unsubstituted and substituted 2-hydroxyacyl-CoA molecules comprising the condensation of formyl-CoA with a carbonyl-containing molecule, wherein the condensation is catalyzed by a prokaryotic HACL. The 2-hydroxyacyl-CoA can be converted to chemical products having broad applications by using enzyme catalysts. The combination of enzyme catalysts comprises novel biochemical reaction pathways that can be deployed either as polypeptides in a reaction buffer or genetically encoded in recombinant microorganisms.Type: ApplicationFiled: November 30, 2021Publication date: May 26, 2022Inventor: Ramon Gonzalez
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Patent number: 11319562Abstract: Methods of using microorganisms to make chemicals and fuels, including carboxylic acids, alcohols, hydrocarbons, and their alpha-, beta-, and omega-functionalized derivatives are described. Native or engineered thiolases are used condense a growing acyl-ACP and acetyl-ACP in combination with type II fatty acid synthesis. The resulting fatty acid biosynthesis cycle has an ATP yield analogous to the functional reverse ?-oxidation cycle.Type: GrantFiled: March 11, 2020Date of Patent: May 3, 2022Inventors: Ramon Gonzalez, James M. Clomburg
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Publication number: 20220112477Abstract: An engineered microbe that contains a designed platform for the conversion of one-carbon substrates to chemical products is described. The designed platform embodies a new metabolic architecture that consolidates carbon fixation, central metabolism, and product synthesis into a single pathway. This is made possible by the key finding that 2-hydroxyacyl-CoA lyase, an enzyme in the ?-oxidation pathway, is capable of catalyzing the C—C bond formation between formyl-CoA and aldehydes of different chain lengths, allowing for the elongation of the carbon backbone of said aldehyde by one-carbon units. These novel microbes present an opportunity for the production of chemicals from single-carbon feedstocks such as carbon dioxide, carbon monoxide, formate, formaldehyde, methanol or methane.Type: ApplicationFiled: October 26, 2021Publication date: April 14, 2022Inventors: Ramon Gonzalez, Alexander Chou, James Clomburg
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Publication number: 20210371884Abstract: This disclosure generally relates to the use of enzyme combinations or recombinant microbes comprising same to make isoprenoid precursors, isoprenoids and derivatives thereof including prenylated aromatic compounds. Novel metabolic pathways exploiting Claisen, aldol, and acyloin condensations are used instead of the natural mevalonate (MVA) pathway or 1-deoxy-d-xylulose 5-phosphate (DXP) pathways for generating isoprenoid precursors such as isopentenyl pyrophosphate (IPP), dimethylallyl pyrophosphate (DMAPP), and geranyl pyrophosphate (GPP). These pathways have the potential for better carbon and or energy efficiency than native pathways. Both decarboxylative and non-carboxylative condensations are utilized, enabling product synthesis from a number of different starting compounds.Type: ApplicationFiled: April 19, 2021Publication date: December 2, 2021Inventors: Ramon GONZALEZ, James M. CLOMGURG, Seokjung CHEONG
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Patent number: 11186834Abstract: An engineered microbe that contains a designed platform for the conversion of one-carbon substrates to chemical products is described. The designed platform embodies a new metabolic architecture that consolidates carbon fixation, central metabolism, and product synthesis into a single pathway. This is made possible by the key finding that 2-hydroxyacyl-CoA lyase, an enzyme in the ?-oxidation pathway, is capable of catalyzing the C—C bond formation between formyl-CoA and aldehydes of different chain lengths, allowing for the elongation of the carbon backbone of said aldehyde by one-carbon units. These novel microbes present an opportunity for the production of chemicals from single-carbon feedstocks such as carbon dioxide, carbon monoxide, formate, formaldehyde, methanol or methane.Type: GrantFiled: October 29, 2015Date of Patent: November 30, 2021Inventors: Ramon Gonzalez, Alexander Chou, James Clomburg
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Publication number: 20210330932Abstract: A catheter including a tubular catheter body defining a distal portion, a distal end and a lumen that extends to the distal end, a radiopaque marker carried within the lumen, and a non-metal tip that is bonded to distal end of the catheter body.Type: ApplicationFiled: July 3, 2021Publication date: October 28, 2021Inventors: Juan Ramon Gonzalez, Sam William Bowman, Rudolph A. Montalvo
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Patent number: 11123516Abstract: A catheter including a tubular catheter body defining a distal portion, a distal end and a lumen that extends to the distal end, a radiopaque marker carried within the lumen, and a non-metal tip that is bonded to distal end of the catheter body.Type: GrantFiled: July 20, 2018Date of Patent: September 21, 2021Assignee: Medtronic Minimed, Inc.Inventors: Juan Ramon Gonzalez, Sam William Bowman, Rudolph A. Montalvo
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Patent number: 11046978Abstract: This disclosure generally relates to the use of enzyme combinations or recombinant microbes comprising same to make isoprenoid precursors, isoprenoids and derivatives thereof including prenylated aromatic compounds. Novel metabolic pathways exploiting Claisen, aldol, and acyioin condensations are used instead of the natural mevalonate (MVA) pathway or 1-deoxy-d-xylulose 5-phosphate (DXP) pathways for generating isoprenoid precursors such as isopentenyl pyrophosphate (IPP), dimethylallyl pyrophosphate (DMAPP), and geranyl pyrophosphate (GPP). These pathways have the potential for better carbon and or energy efficiency than native pathways. Both decarboxylative and non-carboxylative condensations are utilized, enabling product synthesis from a number of different starting compounds.Type: GrantFiled: March 15, 2017Date of Patent: June 29, 2021Assignee: WILLIAM MARSH RICE UNIVERSITYInventors: Ramon Gonzalez, James M. Clomburg, Seokjung Cheong
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Publication number: 20210054427Abstract: This disclosure generally relates to the use of microorganisms to make various functionalized polyketides through polyketoacyl-CoA thiolase-catalyzed non-decarboxylative condensation reactions instead of decarboxylative reactions catalyzed by polyketide synthases. Native or engineered polyketoacyl-CoA thiolases catalyze the non-decarboxylative Claisen condensation in an iterative manner (i.e. multiple rounds) between two either unsubstituted or functionalized ketoacyl-CoAs (and polyketoacyl-CoAs) serving as the primers and acyl-CoAs serving as the extender unit to generate (and elongate) polyketoacyl-CoAs. Before the next round of polyketoacyl-CoA thiolase reaction, the ?-keto group of the polyketide chain of polyketoacyl-CoA can be reduced and modified step-wise by 3-OH-polyketoacyl-CoA dehydrogenase or polyketoenoyl-CoA hydratase or polyketoenoyl-CoA reductase. Dehydrogenase converts the ?-keto group to ?-hydroxy group. Hydratase converts the ?-hydroxy group to ?-?-double-bond.Type: ApplicationFiled: July 15, 2020Publication date: February 25, 2021Inventor: Ramon Gonzalez
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Publication number: 20210002677Abstract: The use of microorganisms to make omega- and/or omega-1-functionalized products through an iterative carbon chain elongation pathway that we call a reverse beta oxidation pathway. The pathway uses omega-functionalized CoA thioesters as primers and acetyl-CoA as the extender unit in a non-decarboxylative Claisen condensation, and then uses beta oxidation or fatty acid synthesis enzymes to complete the cycle, via reductase, dehydratase and reductase reactions. Various termination enzymes that act on the functionalized beta-keto acyl-CoA intermediates of the pathway and produce omega or omega-1 functionalized products. The action of termination enzymes on such intermediates yield a large variety of products.Type: ApplicationFiled: September 15, 2020Publication date: January 7, 2021Inventor: Ramon GONZALEZ
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Patent number: 10858261Abstract: The present invention relates to a method for obtaining calcium aluminates for metallurgical use from non-saline aluminum slags by means of reactive grinding and thermal treatment.Type: GrantFiled: July 26, 2016Date of Patent: December 8, 2020Assignees: ARZYZ, S.A. DE C.V., CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS (CSIC)Inventors: Felix Antonio Lopez Gomez, Francisco Jose Alguacil Priego, Jose Ramon Gonzalez Gracia, Mario Sergio Ramirez Zablah
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Patent number: 10851382Abstract: The present invention relates with the technical field of biotechnology, and particularly provides a promoter inducible under abiotic stress conditions, which is used to regulate expression of a nucleotide sequence encoding a product of interest under these conditions. The invention also refers to a genetic construction containing said promoter, the plant cells transformed with said construction as well as the transgenic plants that can be regenerated from said cells, and which are capable of growing and developing properly, keeping high levels of productive yield under abiotic stress conditions.Type: GrantFiled: October 12, 2016Date of Patent: December 1, 2020Assignees: UNIVERSIDAD DE TALCA, INVERSIONES Y ASESORÏA OLIVARES Y MELOSSI LTDA., INVESTIGACIONES AGRÍCOLAS Y FORESTALES DEL MAULE S.A., FERMELO S.AInventors: Simön Aurelio Ruiz Lara, Enrique Ramón Gonzalez Villanueva, Jorge Luis Pérez Díaz, José Ricardo Pérez Díaz, Mónica Loreto Yañez Chävez, Isabel Alejandra Verdugo Bastías, Sebastián Alejandro González Díaz, Ricardo Javier Chilian
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Publication number: 20200347423Abstract: An engineered microorganism(s) with novel pathways for the conversion of short-chain hydrocarbons to fuels and chemicals (e.g. carboxylic acids, alcohols, hydrocarbons, and their alpha-, beta-, and omega-functionalized derivatives) is described. Key to this approach is the use of hydrocarbon activation enzymes able to overcome the high stability and low reactivity of hydrocarbon compounds through the cleavage of an inert C—H bond. Oxygen-dependent or oxygen-independent activation enzymes can be exploited for this purpose, which when combined with appropriate pathways for the conversion of activated hydrocarbons to key metabolic intermediates, enables the generation of product precursors that can subsequently be converted to desired compounds through established pathways. These novel engineered microorganism(s) provide a route for the production of fuels and chemicals from short chain hydrocarbons such as methane, ethane, propane, butane, and pentane.Type: ApplicationFiled: May 19, 2020Publication date: November 5, 2020Inventors: Ramon Gonzalez, James Clomburg, Alexander Chou
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Publication number: 20200325502Abstract: The use of microorganisms to make alpha-functionalized chemicals and fuels, (e.g. alpha-functionalized carboxylic acids, alcohols, hydrocarbons, amines, and their beta-, and omega-functionalized derivatives), by utilizing an iterative carbon chain elongation pathway that uses functionalized extender units. The core enzymes in the pathway include thiolase, dehydrogenase, dehydratase and reductase. Native or engineered thiolases catalyze the condensation of either unsubstituted or functionalized acyl-CoA primers with an alpha-functionalized acetyl-CoA as the extender unit to generate alpha-functionalized ?-keto acyl-CoA. Dehydrogenase converts alpha-functionalized ?-keto acyl-CoA to alpha-functionalized ?-hydroxy acyl-CoA. Dehydratase converts alpha-functionalized ?-hydroxy acyl-CoA to alpha-functionalized enoyl-CoA. Reductase converts alpha-functionalized enoyl-CoA to alpha-functionalized acyl-CoA. The platform can be operated in an iterative manner (i.e.Type: ApplicationFiled: March 13, 2020Publication date: October 15, 2020Inventors: Ramon GONZALEZ, James M. CLOMBURG, Seokjung CHEONG