Patents by Inventor Randall D. Lowe, JR.
Randall D. Lowe, JR. has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20200400669Abstract: A method of preparing an antibody therapeutic is provided comprising: (a) providing a dissociated cell sample from at least one solid tumor sample obtained from a patient; (b) loading the dissociated cell sample into a microfluidic device having a flow region and at least one isolation region fluidically connected to the flow region; (c) moving at least one B cell from the dissociated cell sample into at least one isolation region in the microfluidic device, thereby obtaining at least one isolated B cell; and (d) using the microfluidic device to identify at least one B cell that produces antibodies capable of binding to cancer cells. The cancer cells can be the patient's own cancer cells. Also provided are methods of treating patients, methods of labeling or detecting cancer, engineered T or NK cells comprising antibodies or fragments thereof, and engineered antibody constructs.Type: ApplicationFiled: June 4, 2020Publication date: December 24, 2020Applicant: Berkeley Lights, Inc.Inventors: Kevin T. Chapman, Mark P. White, Xiaohua Wang, Minha Park, Guido K. Stadler, Randall D. Lowe, JR., Xiao Guan Radstrom, Jason M. McEwen, Gang F. Wang, George L. Fox, Peggy A. Radel
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Patent number: 10799865Abstract: Microfluidic devices having an electrowetting configuration and an optimized droplet actuation surface are provided. The devices include a conductive substrate having a dielectric layer, a hydrophobic layer covalently bonded to the dielectric layer, and a first electrode electrically coupled to the dielectric layer and configured to be connected to a voltage source. The microfluidic devices also include a second electrode, optionally included in a cover, configured to be connected to the voltage source. The hydrophobic layer features self-associating molecules covalently bonded to a surface of the dielectric layer in a manner that produces a densely-packed monolayer that resists intercalation and or penetration by polar molecules or species.Type: GrantFiled: October 27, 2016Date of Patent: October 13, 2020Assignee: Berkeley Lights, Inc.Inventors: Randall D. Lowe, Jr., Shao Ning Pei, Jian Gong, Alexander J. Mastroianni, Jason M. McEwen, Justin K. Valley
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Publication number: 20200299351Abstract: In biosciences and related fields, it can be useful to modify surfaces of apparatuses, devices, and materials that contact biomaterials such as biomolecules and biological micro-objects. Described herein are surface modifying and surface functionalizing reagents, preparation thereof, and methods for modifying surfaces to activate T Lymphocytes.Type: ApplicationFiled: January 15, 2020Publication date: September 24, 2020Applicant: Berkeley Lights, Inc.Inventors: Peter J. Beemiller, Alexander J. Mastroianni, Shao Ning Pei, Randall D. Lowe, JR., Annamaria Mocciaro, Kevin D. Loutherback, Yelena Bronevetsky, Guido K. Stadler, Andrew W. McFarland, Kevin T. Chapman, Duane Smith, Natalie C. Marks, Amanda L. Goodsell
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Patent number: 10723988Abstract: Systems, methods and kits are described for culturing one or more biological cells in a microfluidic device, including provision of nutrients and gaseous components configured to enhance cell growth, viability, portability, or any combination thereof. In some embodiments, culturing a single cell may produce a clonal population in the microfluidic device.Type: GrantFiled: April 22, 2016Date of Patent: July 28, 2020Assignee: Berkeley Lights, Inc.Inventors: Randall D. Lowe, Jr., Kristin Beaumont, Aathavan Karunakaran, Natalie Marks, Jason M. McEwen, Mark P. White, J. Tanner Nevill, Gang F. Wang, Andrew W. McFarland, Daniele Malleo, Keith J. Breinlinger, Xiao Guan, Kevin T. Chapman
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Patent number: 10712344Abstract: A method of preparing an antibody therapeutic is provided comprising: (a) providing a dissociated cell sample from at least one solid tumor sample obtained from a patient; (b) loading the dissociated cell sample into a microfluidic device having a flow region and at least one isolation region fluidically connected to the flow region; (c) moving at least one B cell from the dissociated cell sample into at least one isolation region in the microfluidic device, thereby obtaining at least one isolated B cell; and (d) using the microfluidic device to identify at least one B cell that produces antibodies capable of binding to cancer cells. The cancer cells can be the patient's own cancer cells. Also provided are methods of treating patients, methods of labeling or detecting cancer, engineered T or NK cells comprising antibodies or fragments thereof, and engineered antibody constructs.Type: GrantFiled: January 13, 2017Date of Patent: July 14, 2020Assignee: Berkeley Lights, Inc.Inventors: Kevin T. Chapman, George L. Fox, Peggy A. Radel, Mark P. White, Xiaohua Wang, Minha Park, Guido K. Stadler, Randall D. Lowe, Jr., Xiao Guan Radstrom, Jason M. McEwen, Gang F. Wang
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Publication number: 20200171501Abstract: Microfluidic devices having an electrowetting configuration and an optimized droplet actuation surface are provided for processing biological cells, e.g., for use in nucleic acid library preparation and/or synthesis (including amplification). The devices include a dielectric layer, a hydrophobic layer covalently bonded to the dielectric layer, and a first electrode. Methods of nucleic acid library preparation and/or synthesis can involve providing reagents to cells or nucleic acids by merging appropriate droplets on a droplet actuation surface within a water-immiscible organic liquid and can be performed in the presence of appropriate surfactants. The hydrophobic layer features self-associating molecules covalently bonded to a surface of the dielectric layer in a manner that produces a densely-packed monolayer that resists intercalation and or penetration by polar molecules or species.Type: ApplicationFiled: October 23, 2019Publication date: June 4, 2020Applicant: Berkeley Lights, Inc.Inventors: Jason M. McEwen, Magali Soumillon, Shao Ning Pei, Randall D. Lowe, Jr., Samira A. Nedungadi, Volker L.S. Kurz, Jian Gong, Yara X. Mejia Gonzalez, Mckenzi S. Toh, Brian A. Rabkin, Jason C. Briggs, Darcy K. Kelly-Greene, James M. Porter, Jr.
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Publication number: 20200139362Abstract: Proto-antigen-presenting surfaces and related kits, methods, and uses are provided. The proto-antigen-presenting surface can comprise a plurality of primary activating molecular ligands comprising a major histocompatibility complex (MHC) molecule configured to bind to a T cell receptor (TCR) of a T cell and a plurality of of co-activating molecular ligands each including a TCR co-activating molecule or an adjunct TCR activating molecule, wherein an exchange factor is bound to the MHC molecules. Exchange factors include, e.g., dipeptides such as GL, GF, GR, etc. Proto-antigen-presenting surfaces can be used to rapidly prepare antigen-presenting surfaces comprising one or more peptide antigens of interest by contacting the proto-antigen-presenting surface with one or more peptide antigens so as to displace the exchange factor. As such, the disclosure facilitates rapid evaluation of the immunogenicity of peptide antigens for activating T lymphocytes.Type: ApplicationFiled: October 17, 2019Publication date: May 7, 2020Applicant: Berkeley Lights, Inc.Inventors: Peter J. BEEMILLER, Alexander J. MASTROIANNI, Shao Ning PEI, Randall D. LOWE, Jr., Annamaria MOCCIARO, Kevin D. LOUTHERBACK, Yelena BRONEVETSKY, Guido K. STADLER, Andrew W. MCFARLAND, Kevin T. CHAPMAN, Duane SMITH, Natalie C. MARKS, Amanda L. GOODSELL
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Publication number: 20200123491Abstract: In biosciences and related fields, it can be useful to modify surfaces of apparatuses, devices, and materials that contact biomaterials such as biomolecules and biological micro-objects.Type: ApplicationFiled: September 20, 2019Publication date: April 23, 2020Inventors: Peter J. BEEMILLER, Alexander J. MASTROIANNI, Randall D. LOWE, JR., Yelena BRONEVETSKY
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Publication number: 20200115680Abstract: Methods of expanding T lymphocytes in a microfluidic device are provided. The methods can include introducing one or more T lymphocytes into a microfluidic device; contacting the one or more T lymphocytes with an activating agent; and perfusing culture medium through the microfluidic device for a period of time sufficient to allow the one or more T lymphocytes to undergo at least one round of mitotic cell division. The expansion can be non-specific or antigen-specific. T lymphocytes produced according to the disclosed methods are also provided, along with methods of treating cancer in a subject. The methods of treating cancer can include isolating T lymphocytes from a tissue sample obtained from the subject; expanding the isolated T lymphocytes in a microfluidic device; exporting the expanded T lymphocytes from the microfluidic device; and reintroducing the expanded T lymphocytes into the subject.Type: ApplicationFiled: July 19, 2019Publication date: April 16, 2020Applicant: Berkeley Lights, Inc.Inventors: Yelena Bronevetsky, Xiaohua Wang, Peter J. Beemiller, Kristin G. Beaumont, Randall D. Lowe, JR., Alexander J. Mastroianni, Kevin T. Chapman, Natalie C. Marks
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Publication number: 20200064337Abstract: Methods are described herein for screening an antibody producing cell within a microfluidic environment. The antibody producing cell may be a B cell lymphocyte, which may be a memory B cell or a plasma cell. An antigen of interest may be brought into proximity with the antibody producing cell and binding of the antigen by an antibody produced by the antibody producing cell may be monitored. Methods of obtaining a sequencing library from an antibody producing cell are also described.Type: ApplicationFiled: April 22, 2019Publication date: February 27, 2020Inventors: Minha Park, Jason C. Briggs, Jason M. McEwen, Ravi K. Ramenani, Hariharasudhan Chirra Dinakar, Kai W. Szeto, Adrienne T. Higa, Mark P. White, Randall D. Lowe, JR., Xiaohua Wang, Kevin T. Chapman
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Patent number: 10569271Abstract: Single-sided optoelectrowetting (SSOEW)-configured substrates are provided, as well as microfluidic devices that include such substrates. The substrates can include a planar electrode, a photoconductive (or photosensitive) layer, a dielectric layer (single-layer or composite), a mesh electrode, and a hydrophobic coating. Fluid droplets can be moved across the hydrophobic coating of such substrates in a light-actuated manner, upon the application of a suitable AC voltage potential across the substrate and the focusing of light into the photoconductive layer of the substrate in a location proximal to the droplets. Walls can be disposed upon the substrates to form the microfluidic devices. Together the walls and substrate can form a microfluidic circuit, through which droplets can be moved.Type: GrantFiled: October 17, 2017Date of Patent: February 25, 2020Assignees: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, BERKELEY LIGHTS, INC.Inventors: Ming-Chiang Wu, Jodi Tsu-An Loo, Shao Ning Pei, Gaetan L. Mathieu, Jian Gong, Randall D. Lowe, Jr., Justin K. Valley
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Publication number: 20190275516Abstract: In biosciences and related fields, it can be useful to modify surfaces of apparatuses, devices, and materials that contact biomaterials such as biomolecules and biological micro-objects. Described herein are surface modifying and surface functionalizing reagents, preparation thereof, and methods for modifying surfaces to provide improved or altered performance with biomaterials.Type: ApplicationFiled: November 20, 2018Publication date: September 12, 2019Applicant: Berkeley Lights, Inc.Inventors: Randall D. Lowe, JR., Alexander J. Mastroianni, Mark P. White, Gregory G. Lavieu, Kristin G. Beaumont
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Publication number: 20190240665Abstract: Methods, systems and kits are described herein for detecting the results of an assay. In particular, the methods, systems and devices of the present disclosure rely on a difference between the diffusion rates of a reporter molecule and an analyte of interest in order to quantify an amount of analyte in a microfluidic device. The analyte may be a secreted product of a biological micro-object.Type: ApplicationFiled: October 15, 2018Publication date: August 8, 2019Inventors: Troy A. LIONBERGER, Matthew E. FOWLER, Phillip J. M. ELMS, Kevin D. LOUTHERBACK, Randall D. LOWE, JR., Jian GONG, Tanner J. NEVILL, Gang F. WANG, Gregory G. LAVIEU, John A. TENNEY, Aathavan KARUNAKARAN
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Publication number: 20190064038Abstract: Aspects of the present disclosure are directed to the manipulation of a cell nucleus in a micro-fluidic device as well as compositions, systems, and kits for performing such methods. In some aspects, the disclosure provides methods for placing one or more selected cell nuclei into an isolation region of a sequestration pen in a micro-fluidic device. The isolated nucleus/nuclei may then be retrieved from the isolation region of the sequestration pen and used in any desired downstream assay or process.Type: ApplicationFiled: September 4, 2018Publication date: February 28, 2019Inventors: Mark P. White, Randall D. Lowe, Jr.
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Patent number: 10101250Abstract: Aspects of the present disclosure are directed to the manipulation of a cell nucleus in a micro-fluidic device as well as compositions, systems, and kits for performing such methods. In some aspects, the disclosure provides methods for placing one or more selected cell nuclei into an isolation region of a sequestration pen in a micro-fluidic device. The isolated nucleus/nuclei may then be retrieved from the isolation region of the sequestration pen and used in any desired downstream assay or process.Type: GrantFiled: April 22, 2016Date of Patent: October 16, 2018Assignee: Berkeley Lights, Inc.Inventors: Mark P. White, Randall D. Lowe, Jr.
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Patent number: 10056314Abstract: A polymer thermal interface material is described that has enhanced thermal conductivity. In one example, a vinyl-terminated silicone oil is combined with a silicone chain extender, and a thermally conductive filler comprising at least 85% by weight of the material, and comprising surface wetted particles with a range of shapes and sizes. The material may be used for bonding components inside a microelectronic package, for example.Type: GrantFiled: June 10, 2015Date of Patent: August 21, 2018Assignee: Intel CorporationInventors: Randall D. Lowe, Jr., Syadwad Jain, James C. Matayabas, Jr.
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Publication number: 20180135011Abstract: Methods of expanding T lymphocytes in a microfluidic device are provided. The methods can include introducing one or more T lymphocytes into a microfluidic device; contacting the one or more T lymphocytes with an activating agent; and perfusing culture medium through the microfluidic device for a period of time sufficient to allow the one or more T lymphocytes to undergo at least one round of mitotic cell division. The expansion can be non-specific or antigen-specific. T lymphocytes produced according to the disclosed methods are also provided, along with methods of treating cancer in a subject. The methods of treating cancer can include isolating T lymphocytes from a tissue sample obtained from the subject; expanding the isolated T lymphocytes in a microfluidic device; exporting the expanded T lymphocytes from the microfluidic device; and reintroducing the expanded T lymphocytes into the subject.Type: ApplicationFiled: November 2, 2017Publication date: May 17, 2018Inventors: Yelena Bronevetsky, Xiaohua Wang, Peter J. Beemiller, Kristin G. Beaumont, Randall D. Lowe, JR., Alexander J. Mastroianni, Kevin T. Chapman
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Publication number: 20180099275Abstract: Single-sided optoelectrowetting (SSOEW)-configured substrates are provided, as well as microfluidic devices that include such substrates. The substrates can include a planar electrode, a photoconductive (or photosensitive) layer, a dielectric layer (single-layer or composite), a mesh electrode, and a hydrophobic coating. Fluid droplets can be moved across the hydrophobic coating of such substrates in a light-actuated manner, upon the application of a suitable AC voltage potential across the substrate and the focusing of light into the photoconductive layer of the substrate in a location proximal to the droplets. Walls can be disposed upon the substrates to form the microfluidic devices. Together the walls and substrate can form a microfluidic circuit, through which droplets can be moved.Type: ApplicationFiled: October 17, 2017Publication date: April 12, 2018Applicants: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, BERKELEY LIGHTS, INC.Inventors: Ming-Chiang Wu, Jodi Tsu-An Loo, Shao Ning Pei, Gaetan L. Mathieu, Jian Gong, Randall D. Lowe, JR., Justin K. Valley
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Patent number: 9815056Abstract: Single-sided optoelectrowetting (SSOEW)-configured substrates are provided, as well as microfluidic devices that include such substrates. The substrates can include a planar electrode, a photoconductive (or photosensitive) layer, a dielectric layer (single-layer or composite), a mesh electrode, and a hydrophobic coating. Fluid droplets can be moved across the hydrophobic coating of such substrates in a light-actuated manner, upon the application of a suitable AC voltage potential across the substrate and the focusing of light into the photoconductive layer of the substrate in a location proximal to the droplets. Walls can be disposed upon the substrates to form the microfluidic devices. Together the walls and substrate can form a microfluidic circuit, through which droplets can be moved.Type: GrantFiled: December 4, 2015Date of Patent: November 14, 2017Assignees: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, BERKELEY LIGHTS, INC.Inventors: Ming-Chiang Wu, Jodi Tsu-An Loo, Shao Ning Pei, Gaetan L. Mathieu, Jian Gong, Randall D. Lowe, Jr., Justin K. Valley
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Publication number: 20170276679Abstract: A method of preparing an antibody therapeutic is provided comprising: (a) providing a dissociated cell sample from at least one solid tumor sample obtained from a patient; (b) loading the dissociated cell sample into a microfluidic device having a flow region and at least one isolation region fluidically connected to the flow region; (c) moving at least one B cell from the dissociated cell sample into at least one isolation region in the microfluidic device, thereby obtaining at least one isolated B cell; and (d) using the microfluidic device to identify at least one B cell that produces antibodies capable of binding to cancer cells. The cancer cells can be the patient's own cancer cells. Also provided are methods of treating patients, methods of labeling or detecting cancer, engineered T or NK cells comprising antibodies or fragments thereof, and engineered antibody constructs.Type: ApplicationFiled: January 13, 2017Publication date: September 28, 2017Inventors: Kevin T. Chapman, Mark P. White, Xiaohua Wang, Minha Park, Guido K. Stadler, Randall D. Lowe, Jr., Xiao Guan, Jason M. McEwen, Gang Wang, George L. Fox, Peggy A. Radel