Patents by Inventor Randall E. Bolger

Randall E. Bolger has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Kinase and phosphatase activity measurement using fluorescence polarization
    Publication number: 20030036106
    Abstract: A method for quantitating enzyme activity that causes phosphorylation or dephosphorylation of a peptide or protein substrate is provided. The method utilizes the differences in the fluorescence polarization of a phosphorylated amino acid-containing fluorescence-emitting reporter molecule free in solution as compared to being bound to a binding molecule.
    Type: Application
    Filed: April 26, 2002
    Publication date: February 20, 2003
    Inventors: Thomas J. Burke, Randall E. Bolger, Rebecca Schall, Gregory Parker
  • Method for quantitating competitive binding of molecules to proteins utilizing fluorescence polarization
    Patent number: 6511815
    Abstract: The system comprises mixing a fluorescence-emitting compound that binds to the steroid hormone receptors in a solution containing the steroid hormone receptors. Then, measuring the fluorescence polarization of the solution. Subsequently, incubating the solution with at least one molecule that may compete with the compound for interaction with the steroid hormone receptors. Measuring the fluorescence polarization of the solution again. Finally, comparing the fluorescence polarization measurements to quantify any competitive interaction.
    Type: Grant
    Filed: December 20, 1999
    Date of Patent: January 28, 2003
    Assignee: PanVera LLC
    Inventors: Thomas J. Burke, Randall E. Bolger, Kerry M. Ervin, Robert G. Lowery, William J. Checovich
  • KINASE ACTIVITY MEASUREMENT USING FLUORESCENCE POLARIZATION
    Publication number: 20010004522
    Abstract: A method for quantitating enzyme activity of phosphorylation and dephosphorylation of a peptide or protein substrate, comprising measuring the fluorescence polarization of a fluorescence-emitting reporter molecule in solution with the phosphate. Then, adding an enzyme, either a kinase or a phosphatase, and incubating the solution. Finally, measuring the fluorescence polarization of the solution after the enzyme has had an opportunity to react with the peptide or protein substrate.
    Type: Application
    Filed: October 28, 1997
    Publication date: June 21, 2001
    Inventors: THOMAS J. BURKE, RANDALL E. BOLGER, REBECCA SCHALL, GREGORY PARKER
  • Process for reverse transcriptase activity measurement using fluorescence polarization
    Patent number: 6100039
    Abstract: Described is a process for detecting reverse transcriptase activity and, thereby, reverse transcriptase inhibitors using fluorescence polarization, comprising, mixing a DNA primer with an RNA template. Then forming an RNA/DNA duplex utilizing the reverse transcriptase and removing the RNA from the RNA/DNA duplex to form single-stranded DNA. Finally, adding a fluorescent-labeled oligonucleotide complementary to the single-stranded DNA for hybridizing to the single-stranded DNA; and, measuring the fluorescence polarization.
    Type: Grant
    Filed: January 28, 1999
    Date of Patent: August 8, 2000
    Assignee: Panvera, Inc.
    Inventors: Thomas J. Burke, Randall E. Bolger, Francis J. Lenoch
  • Method for detecting and quantitating nucleic acid impurities in biochemical preparations
    Patent number: 5824557
    Abstract: A homogeneous fluorescence-based nucleic acid detection and quantitation system is provided to measure nucleic acid in protein solutions. The process relies on the intercalation of a non-fluorescent dye into a double-stranded nucleic acid helix or single-stranded nucleic acid. The dye fluoresces after intercalation and the intensity is a direct measurement of the amount of nucleic acid present in the sample.
    Type: Grant
    Filed: April 2, 1996
    Date of Patent: October 20, 1998
    Assignee: PanVera Corporation
    Inventors: Thomas J. Burke, Randall E. Bolger, Francis J. Lenoch
  • Method and kit for detecting nucleic acid cleavage utilizing a covalently attached fluorescent tag
    Patent number: 5786139
    Abstract: The present invention is based on the degradation of a fluorescence labeled nucleic acid such as DNA (F-DNA), RNA (F-RNA) or DNA/RNA substrate. The label may be covalently attached to the relatively high volume substrate at one position or many positions. The nucleic acid is then put in contact with another substance and activity is detected by Fluorescence Polarization.
    Type: Grant
    Filed: December 9, 1994
    Date of Patent: July 28, 1998
    Assignee: PanVera Corporation
    Inventors: Thomas J. Burke, Randall E. Bolger, William J. Checovich, David V. Thompson