Abstract: The present invention is directed to method of isolating an antibody from a composition. In some embodiments, the method comprises isolating Synagis® from a composition comprising Synagis®, the method comprising: (i) performing an ion exchange chromatography process on the composition; (ii) performing an affinity purification process on the composition; and (iii) performing a filtration process on the composition, wherein a final product comprising Synagis® results from (i), (ii), and (iii), wherein the final product is suitable for administration to a human and has a DNA concentration of <0.5 pg/mg, and wherein the method does not comprise adding benzonase to the composition.

Abstract: A polynucleotide sequence is provided, comprising: a nucleic acid sequence having at least 75% identity to SEQ ID NO: 1, wherein said nucleic acid sequence encodes recombinant Bacillus anthracis Protective Antigen (rPA); or a fragment of said nucleic acid sequence wherein said fragment encodes a fragment of recombinant Bacillus anthracis Protective Antigen (rPA). Also provided are expression vectors and host cells comprising the polynucleotide sequence of the invention, and methods for producing rPA or a fragment thereof. The invention further provides antigenic compositions and corresponding methods and uses for inducing an immune response.