Abstract: A method of preparing an antibody therapeutic is provided comprising: (a) providing a dissociated cell sample from at least one solid tumor sample obtained from a patient; (b) loading the dissociated cell sample into a microfluidic device having a flow region and at least one isolation region fluidically connected to the flow region; (c) moving at least one B cell from the dissociated cell sample into at least one isolation region in the microfluidic device, thereby obtaining at least one isolated B cell; and (d) using the microfluidic device to identify at least one B cell that produces antibodies capable of binding to cancer cells. The cancer cells can be the patient's own cancer cells. Also provided are methods of treating patients, methods of labeling or detecting cancer, engineered T or NK cells comprising antibodies or fragments thereof, and engineered antibody constructs.

Abstract: A clock alignment system includes a first clock generator generating a first clock signal in a first clock domain and a second clock generator generating a second clock signal in a second clock domain slower than the first clock domain. A coarse delay-locked loop (DLL) generates third clock signals having corresponding phase offsets from the first clock signal, and a fine DLL generates a fourth clock signal by adjusting the phase of a selected one of the third clock signals. The second clock generator generates the second clock signal from the fourth clock signal. A phase detector compares phases of the first and second clock signals. A control circuit aligns the first and second clock signals by using the compared phases to select the third clock signal output by the coarse DLL, and control the phase adjustment by the fine DLL of this third clock signal.

Abstract: An analog to digital converter is disclosed that is designed to receive a differential analog signal and includes a signal chopping circuit and a successive approximation register (SAR) coupled to the signal chopping circuit. The signal chopping circuit is designed to invert a polarity of the differential analog signal and includes a first switching circuit having a first transistor and a second switching circuit having a second transistor. A gate of the first transistor and a gate of the second transistor is each coupled to a same bootstrap capacitor. Coupling both switching circuits to the same bootstrap capacitor (as opposed to separate bootstrap capacitors) greatly frees up space on the die or chip.

Abstract: A dynamic differential amplifier includes: gain transistors to drive with differential input voltage levels; sample capacitors having first terminals to ramp from an initial voltage level to differential amplified voltage levels of the input voltage levels in response to the driven gain transistors; and adjustment circuits to adjust the amplified voltage levels in the direction of the initial voltage level by an offset voltage level. In some cases, second terminals of the sample capacitors are a common-mode node to maintain a common-mode voltage level midway between the ramping voltage levels of the first terminals. In some cases, the dynamic differential amplifier further includes a comparison circuit to compare the maintained common-mode voltage level to a threshold voltage level, wherein the first terminals of the sample capacitors stop ramping and the adjustment circuits adjust the amplified voltage levels in response to the compared common-mode voltage level reaching the threshold voltage level.

Abstract: Circuit techniques are disclosed for improving the SFDR of a DAC. In an embodiment, a DAC includes a resistor ladder network operably coupled to input logic circuitry and an output. The input logic circuitry receives a multi-bit input signal and effectively creates a plurality of processed input signals therefrom. The resistor ladder network is configured to receive the plurality of processed input signals and includes a corresponding plurality of current paths. Each current path includes: a current switch operably controlled by one of the processed input signals; a first resistor in series with the current switch; a second resistor in series with the first resistor; and a feedforward capacitor in parallel with the second resistor. The output is operably coupled to each of the plurality of current paths and is configured to output an analog output signal that corresponds to the multi-bit input signal.

Abstract: A dynamic differential amplifier includes: gain transistors to drive with differential input voltage levels; sample capacitors having first terminals to ramp from an initial voltage level to differential amplified voltage levels of the input voltage levels in response to the driven gain transistors; and adjustment circuits to adjust the amplified voltage levels in the direction of the initial voltage level by an offset voltage level. In some cases, second terminals of the sample capacitors are a common-mode node to maintain a common-mode voltage level midway between the ramping voltage levels of the first terminals. In some cases, the dynamic differential amplifier further includes a comparison circuit to compare the maintained common-mode voltage level to a threshold voltage level, wherein the first terminals of the sample capacitors stop ramping and the adjustment circuits adjust the amplified voltage levels in response to the compared common-mode voltage level reaching the threshold voltage level.