Abstract: Systems, methods and kits are described for culturing one or more biological cells in a microfluidic device, including provision of nutrients and gaseous components configured to enhance cell growth, viability, portability, or any combination thereof. In some embodiments, culturing a single cell may produce a clonal population in the microfluidic device.

Abstract: In biosciences and related fields, it can be useful to modify surfaces of apparatuses, devices, and materials that contact biomaterials such as biomolecules and biological micro-objects. Described herein are surface modifying and surface functionalizing reagents, preparation thereof, and methods for modifying surfaces to activate T Lymphocytes.

Abstract: A multi-fuel rail apparatus for an internal combustion engine communicates fuel from 10 a first fuel source and a second fuel source to a plurality of fuel injectors. Each fuel injector receives fuel from the multi-fuel rail apparatus through a branch connection for each fuel. The multi fuel rail apparatus has a first elongate member including a first longitudinal bore spaced apart from a second longitudinal bore and first and second fuel inlets for fluidly communicating first and second fuels into the first and 15 second longitudinal bores respectively. There is a branch connecting structure for each fuel injector along the first elongate member for fluidly connecting the first and second longitudinal bores with respective branch connections from respective fuel injectors.

Abstract: A multi-fuel rail apparatus for an internal combustion engine communicates fuel from 10 a first fuel source and a second fuel source to a plurality of fuel injectors. Each fuel injector receives fuel from the multi-fuel rail apparatus through a branch connection for each fuel. The multi fuel rail apparatus has a first elongate member including a first longitudinal bore spaced apart from a second longitudinal bore and first and second fuel inlets for fluidly communicating first and second fuels into the first and 15 second longitudinal bores respectively. There is a branch connecting structure for each fuel injector along the first elongate member for fluidly connecting the first and second longitudinal bores with respective branch connections from respective fuel injectors.

Abstract: A module for controlling a first fuel rail pressure in an internal combustion engine and maintaining a predetermined pressure bias with a second fuel rail pressure comprises a manifold body and several components that are mounted on the manifold body to form an unitary and independent structure that is fluidly connected to a first fuel supply and to a first fuel rail. The components that are mounted on the manifold body are a pressure regulator, a shut-off valve and a check valve and can further comprise, for example, a service valve for venting the fuel from the module and/or a sensor for measuring the pressure in the first fuel rail.