Abstract: Systems, methods and kits are described for culturing one or more biological cells in a microfluidic device, including provision of nutrients and gaseous components configured to enhance cell growth, viability, portability, or any combination thereof. In some embodiments, culturing a single cell may produce a clonal population in the microfluidic device.

Abstract: Proto-antigen-presenting surfaces and related kits, methods, and uses are provided. The proto-antigen-presenting surface can comprise a plurality of primary activating molecular ligands comprising a major histocompatibility complex (MHC) molecule configured to bind to a T cell receptor (TCR) of a T cell and a plurality of co-activating molecular ligands each including a TCR co-activating molecule or an adjunct TCR activating molecule, wherein an exchange factor is bound to the MHC molecules. Exchange factors include, e.g., dipeptides such as GL, GF, GR, etc. Proto-antigen-presenting surfaces can be used to rapidly prepare antigen-presenting surfaces comprising one or more peptide antigens of interest by contacting the proto-antigen-presenting surface with one or more peptide antigens so as to displace the exchange factor. As such, the disclosure facilitates rapid evaluation of the immunogenicity of peptide antigens for activating T lymphocytes.

Abstract: A multi-fuel rail apparatus for an internal combustion engine communicates fuel from 10 a first fuel source and a second fuel source to a plurality of fuel injectors. Each fuel injector receives fuel from the multi-fuel rail apparatus through a branch connection for each fuel. The multi fuel rail apparatus has a first elongate member including a first longitudinal bore spaced apart from a second longitudinal bore and first and second fuel inlets for fluidly communicating first and second fuels into the first and 15 second longitudinal bores respectively. There is a branch connecting structure for each fuel injector along the first elongate member for fluidly connecting the first and second longitudinal bores with respective branch connections from respective fuel injectors.

Abstract: A multi-fuel rail apparatus for an internal combustion engine communicates fuel from 10 a first fuel source and a second fuel source to a plurality of fuel injectors. Each fuel injector receives fuel from the multi-fuel rail apparatus through a branch connection for each fuel. The multi fuel rail apparatus has a first elongate member including a first longitudinal bore spaced apart from a second longitudinal bore and first and second fuel inlets for fluidly communicating first and second fuels into the first and 15 second longitudinal bores respectively. There is a branch connecting structure for each fuel injector along the first elongate member for fluidly connecting the first and second longitudinal bores with respective branch connections from respective fuel injectors.

Abstract: A module for controlling a first fuel rail pressure in an internal combustion engine and maintaining a predetermined pressure bias with a second fuel rail pressure comprises a manifold body and several components that are mounted on the manifold body to form an unitary and independent structure that is fluidly connected to a first fuel supply and to a first fuel rail. The components that are mounted on the manifold body are a pressure regulator, a shut-off valve and a check valve and can further comprise, for example, a service valve for venting the fuel from the module and/or a sensor for measuring the pressure in the first fuel rail.