Patents by Inventor Ranganathan Godavarti
Ranganathan Godavarti has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9428546Abstract: The present disclosure provides methods for purifying products from a fluid. In some embodiments, provided purification methods use a combination of purification modes (e.g., protein A and ion exchange) operated in tandem, wherein at least one of the modes utilizes weak partitioning. In some embodiments, provided purification methods operate under robust conditions in which a degree of binding between a product and resin is maintained despite variations in operating parameters.Type: GrantFiled: July 29, 2011Date of Patent: August 30, 2016Assignee: Pfizer Inc.Inventors: Jonathan Lee Coffman, Ranganathan Godavarti, Michael Shamashkin
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Patent number: 9144755Abstract: This invention relates to methods of using weak partitioning chromatography for the purification of a product from a load fluid containing one or more impurities. Further, the invention relates to methods of weak partitioning chromatography defined by operating conditions which cause a medium to bind least 1 mg of product per mL of medium, or alternatively, defined by a partition coefficient of at least 0.1.Type: GrantFiled: November 28, 2011Date of Patent: September 29, 2015Assignee: Wyeth LLCInventors: Paul R. Brown, Jonathan Lee Coffman, Ranganathan Godavarti, Timothy Iskra, Brian D. Kelley, Suresh Vunnum, Shujun Sun, Tianning Yu, James Edward Booth, Mary Beth Switzer
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Publication number: 20130317198Abstract: The present disclosure provides methods for purifying products from a fluid. In some embodiments, provided purification methods use a combination of purification modes (e.g., protein A and ion exchange) operated in tandem, wherein at least one of the modes utilizes weak partitioning. In some embodiments, provided purification methods operate under robust conditions in which a degree of binding between a product and resin is maintained despite variations in operating parameters.Type: ApplicationFiled: July 29, 2011Publication date: November 28, 2013Applicant: Pfizer Inc.Inventors: Jonathan Lee Coffman, Ranganathan Godavarti, Michael Shamashkin
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Patent number: 8440799Abstract: The present application provides methods of purifying A? binding proteins having a Fc region, for example, anti-A? antibodies or antibody fusions, by adsorbing the A? binding protein to a Fc binding agent, such as, for example, Protein A or Protein G, followed by a wash with a divalent cation salt buffer to remove impurities and subsequent recovery of the adsorbed A? binding protein. The present application also features methods of eluting the purified A? binding protein as well as the incorporation of the methods within a purification train. Kits comprising components for carrying out the methods and instructions for use are also provided.Type: GrantFiled: October 12, 2010Date of Patent: May 14, 2013Assignees: Janssen Alzheimer Immunotherapy, Wyeth LLCInventors: Ranganathan Godavarti, Timothy Iskra
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Publication number: 20120138537Abstract: This invention relates to methods of using weak partitioning chromatography for the purification of a product from a load fluid containing one or more impurities. Further, the invention relates to methods of weak partitioning chromatography defined by operating conditions which cause a medium to bind least 1 mg of product per mL of medium, or alternatively, defined by a partition coefficient of at least 0.1.Type: ApplicationFiled: November 28, 2011Publication date: June 7, 2012Applicant: Wyeth LLCInventors: Brian D. Kelley, James E. Booth, Paul Brown, Jon Coffman, Ranganathan Godavarti, Tim Iskra, Shujun Sun, Mary B. Switzer, Suresh Vunnum, Tianning Yu
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Patent number: 8067182Abstract: This invention relates to methods of using weak partitioning chromatography for the purification of a product from a load fluid containing one or more impurities. Further, the invention relates to methods of weak partitioning chromatography defined by operating conditions which cause a medium to bind least 1 mg of product per mL of medium, or alternatively, defined by a partition coefficient of at least 0.1.Type: GrantFiled: March 10, 2006Date of Patent: November 29, 2011Assignee: Wyeth LLCInventors: Brian D. Kelley, James E. Booth, Paul Brown, Jon Coffman, Ranganathan Godavarti, Tim Iskra, Shujun Sun, Mary B. Switzer, Suresh Vunnum, Tianning Yu
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Publication number: 20110160437Abstract: The present application provides methods of purifying A? binding proteins having a Fc region, for example, anti-A? antibodies or antibody fusions, by adsorbing the A? binding protein to a Fc binding agent, such as, for example, Protein A or Protein G, followed by a wash with a divalent cation salt buffer to remove impurities and subsequent recovery of the adsorbed A? binding protein. The present application also features methods of eluting the purified A? binding protein as well as the incorporation of the methods within a purification train. Kits comprising components for carrying out the methods and instructions for use are also provided.Type: ApplicationFiled: October 12, 2010Publication date: June 30, 2011Applicants: Janssen Alzheimer Immunotherapy, Wyeth LLCInventors: Ranganathan Godavarti, Timothy Iskra
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Patent number: 7825223Abstract: The present application provides methods of purifying A? binding proteins having a Fc region, for example, anti-A? antibodies or antibody fusions, by adsorbing the A? binding protein to a Fc binding agent, such as, for example, Protein A or Protein G, followed by a wash with a divalent cation salt buffer to remove impurities and subsequent recovery of the adsorbed A? binding protein. The present application also features methods of eluting the purified A? binding protein as well as the incorporation of the methods within a purification train. Kits comprising components for carrying out the methods and instructions for use are also provided.Type: GrantFiled: June 16, 2006Date of Patent: November 2, 2010Assignees: Janssen Alzheimer Immunotherapy, Wyeth LLCInventors: Ranganathan Godavarti, Timothy Iskra
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Patent number: 7820799Abstract: The present application provides methods of purifying polypeptides having a Fc region, for example, antibodies or antibody fusions, by adsorbing the polypeptides to a Fc binding agent, such as, for example, Protein A or Protein G, followed by a wash with a divalent cation salt buffer to remove impurities and subsequent recovery of the adsorbed polypeptides. The present application also features methods of eluting the purified polypeptide as well as the incorporation of the methods within a purification train. Kits comprising components for carrying out the methods and instructions for use are also provided.Type: GrantFiled: June 16, 2006Date of Patent: October 26, 2010Assignees: Janssen Alzheimer Immunotherapy, Wyeth LLCInventors: Ranganathan Godavarti, Timothy Iskra
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Publication number: 20070082367Abstract: The present application provides methods of purifying A? binding proteins having a Fc region, for example, anti-A? antibodies or antibody fusions, by adsorbing the A? binding protein to a Fc binding agent, such as, for example, Protein A or Protein G, followed by a wash with a divalent cation salt buffer to remove impurities and subsequent recovery of the adsorbed A? binding protein. The present application also features methods of eluting the purified A? binding protein as well as the incorporation of the methods within a purification train. Kits comprising components for carrying out the methods and instructions for use are also provided.Type: ApplicationFiled: June 16, 2006Publication date: April 12, 2007Inventors: Ranganathan Godavarti, Timothy Iskra
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Publication number: 20070072307Abstract: The present application provides methods of purifying polypeptides having a Fc region, for example, antibodies or antibody fusions, by adsorbing the polypeptides to a Fc binding agent, such as, for example, Protein A or Protein G, followed by a wash with a divalent cation salt buffer to remove impurities and subsequent recovery of the adsorbed polypeptides. The present application also features methods of eluting the purified polypeptide as well as the incorporation of the methods within a purification train. Kits comprising components for carrying out the methods and instructions for use are also provided.Type: ApplicationFiled: June 16, 2006Publication date: March 29, 2007Inventors: Ranganathan Godavarti, Timothy Iskra
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Publication number: 20070060741Abstract: This invention relates to methods of using weak partitioning chromatography for the purification of a product from a load fluid containing one or more impurities. Further, the invention relates to methods of weak partitioning chromatography defined by operating conditions which cause a medium to bind least 1 mg of product per mL of medium, or alternatively, defined by a partition coefficient of at least 0.1.Type: ApplicationFiled: March 10, 2006Publication date: March 15, 2007Applicant: WyethInventors: Brian Kelley, James Booth, Paul Brown, Jon Coffman, Ranganathan Godavarti, Tim Iskra, Shujun Sun, Mary Switzer, Suresh Vunnum, Tianning Yu
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Publication number: 20070054390Abstract: This invention relates to methods of using weak partitioning chromatography for the purification of a product from a load fluid. Further, the invention relates to methods of weak partitioning chromatography defined by operating conditions which cause a medium to bind least 1 mg of product per mL of medium, or alternatively, defined by a partition coefficient of at least 0.1.Type: ApplicationFiled: August 28, 2006Publication date: March 8, 2007Applicant: WyethInventors: Brian Kelley, James Booth, Paul Brown, Jon Coffman, Ranganathan Godavarti, Tim Iskra, Shujun Sun, Mary Switzer, Suresh Vunnum, Tianning Yu
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Patent number: 6217863Abstract: Modified heparinases having altered binding specificity and activity are provided. Isolated nucleic acids encoding the same as as vectors and host cells are provided. Methods for using the modified heparinases are also provided.Type: GrantFiled: January 19, 1999Date of Patent: April 17, 2001Assignee: Massachusetts Institute of TechnologyInventors: Ranganathan Godavarti, Ram Sasisekharan, Steffen Ernst, Ganesh Venkataraman, Charles L. Cooney, Robert Langer