Abstract: The present invention relates to the field of cancer. More specifically, the present invention provides methods and compositions useful for detecting and treating prostate cancer. In a specific embodiment, a method for identifying a patient as having aggressive prostate cancer comprising the step of detecting overexpression relative to a control of epithelial cell adhesion molecule (EpCAM), H4 clustered histone 5 (H4C5), and tetratricopeptide repeat domain 3 (TTC3) in a urine sample obtained from the patient. In a more specific embodiment, the detecting step comprises detecting protein or ribonucleic acid (RNA) level of EPCAM, H4C5 and TTC3. In another specific embodiment, the method further comprises detecting overexpression relative to a control of one or more of messenger ribonucleic acid (mRNA), circulating RNA (circRNA), extracellular DNA and long non-coding RNA (lncRNA). The method can further comprise detecting metabolites.

Abstract: Methods and compositions are provided for detecting, diagnosing and evaluating presence of prostate cancer in a biological sample.