Abstract: The present invention relates to binding agents of human Leucine-rich Repeat Kinase 2 (LRRK2). More particular, allosteric modulators of LRRK2 activity have been identified, for targeting LRRK2 in human cells, while leaving LRRK2 subcellular localisation unaffected. Even more specifically, protein binding agents for allosteric modulation of LRRK2 kinase activity are disclosed, comprising immunoglobulin single variable domains (ISVDs) binding to human LRRK2 with nanomolar affinity. The invention thus reveals means and methods for a novel LRRK2 targeting approach through allosteric modulation of its activity for use in treatment of LRRK2-related pathologies, such as Parkinson's disease, as well as for use in detection of LRRK2 in vitro and in vivo, and for use as a diagnostic.

Abstract: The present disclosure relates to organic compound(s) (i.e., compound of formula I or compound of formula IA) and/or a composition including the organic compound(s) useful for utilization of non-coking coal in a blend including coking coal without deterioration of properties of coking coal, wherein the organic compound(s) is capable of releasing hydrogen in the plastic region of coal. The present disclosure provides a simple, economical, non-toxic and efficient method for the preparation of the organic polymer, and a method for utilization of non-coking coal in blend including coking coal without deterioration of the properties of coke.