Abstract: The current invention discloses a method for obtaining a purified antibody composition. The method discloses the use of various chromatography steps in a particular order to obtain a purified composition of a therapeutic monoclonal antibody starting from a composition comprising the monoclonal antibody and one or more process and product related impurities. Further, the method also discloses the use of additional purification steps such as depth filtration, diafiltration, ultrafiltration and tangential flow filtration for obtaining the said purified antibody composition.

Abstract: The method disclosed in the current invention is used to purify an antibody from high molecular weight aggregates. The method discloses the use of anion exchange chromatography for the reduction of high molecular weight aggregates from the antibody composition, in particular, by contacting the antibody composition with the anion exchange resin at a specific pH and conductivity. The disclosed method eliminates the need for further chromatographic steps for the reduction of HMW aggregates.