Abstract: Disclosed are a Lactobacillus rhamnosus strain having accession number CCTCC NO: M 2021185, a ferment lysate prepared thereby for regulating skin microecology, and a preparation method for the ferment lysate. The ferment lysate comprises the following components in mass percentages of same in the ferment lysate: 0.2-1% of proteins, 0.3-0.8% of polysaccharides, and 0.2-1% of amino acids. The ferment lysate obtained by using the above bacterial strain for fermentation can strongly inhibit the reproduction and growth of pathogenic bacteria, has a promotion effect on some of probiotics, has a strong inhibition effect on pathogenic hyphae of Candida albicans, can not only generate antimicrobial peptides during fermentation, but also increase the expression level of antimicrobial peptides in skin cells at the genetic level and the protein level, thereby improving skin immunity.

Abstract: A heparin skeleton synthase originates from Neisseria animaloris, with an amino acid sequence as shown in SEQ ID NO.2 and a nucleotide sequence of the coding gene as shown in SEQ ID NO.1. Its recombinant expression level is 6.8 times that of the existing heparin skeleton synthase KfiA from Escherichia coli K5, and total enzyme activity per fermentation liquor is 5.22 times that of the heparin skeleton synthase KfiA. The heparin skeleton synthase mutants obtained through site-directed mutagenesis of the sites No. 16, No. 25, No. 30, No. 111, No. 165, and No. 172 in the amino acid sequence of the said heparin skeleton synthase all have high expression levels.

Abstract: The present application provides a use of a hyaluronic acid or a salt thereof and/or trehalose in stabilizing ergothioneine, an additive for stabilizing ergothioneine, the additive containing the hyaluronic acid or the salt thereof and/or trehalose, and a composition, the composition containing ergothioneine, the hyaluronic acid or the salt thereof, and trehalose. Compared with other auxiliary materials, the hyaluronic acid or the salt thereof and trehalose provided by the present invention can obviously mitigate the damage of a high temperature to ergothioneine, thereby increasing the yield of an ergothioneine powder product. The composition obtained by the present invention has good antioxidant and anti-apoptotic effects.

Abstract: A heparin skeleton synthase originates from Neisseria animaloris, with an amino acid sequence as shown in SEQ ID NO.2 and a nucleotide sequence of the coding gene as shown in SEQ ID NO.1. Its recombinant expression level is 6.8 times that of the existing heparin skeleton synthase KfiA from Escherichia coli K5, and total enzyme activity per fermentation liquor is 5.22 times that of the heparin skeleton synthase KfiA. The heparin skeleton synthase mutants obtained through site-directed mutagenesis of the sites No. 16, No. 25, No. 30, No. 111, No. 165, and No. 172 in the amino acid sequence of the said heparin skeleton synthase all have high expression levels.

Abstract: The present disclosure provides example methods and apparatuses for implementing comment ranking by a search engine. One or more terms describing a target object are extracted from a comment in connection with the target object. One or more useful attributes included in the comment are obtained according to the terms describing the target object. An scoring index the comment is determined according to a number of the useful attributes. The comment is scored according to the index. The comment is ranked according to the score. The present techniques enable a user to quickly and efficiently view helpful comments and help the user make an informed decision.

Abstract: The present disclosure provides example methods and apparatuses for implementing comment ranking by a search engine. One or more terms describing a target object are extracted from a comment in connection with the target object. One or more useful attributes included in the comment are obtained according to the terms describing the target object. An scoring index the comment is determined according to a number of the useful attributes. The comment is scored according to the index. The comment is ranked according to the score. The present techniques enable a user to quickly and efficiently view helpful comments and help the user make an informed decision.