Abstract: The invention relates to a coupling 10 for transferring fluids, having a valve 30, the valve tappet 32 of which is coupled in terms of movement with a valve sleeve 31, which is pre-tensioned by spring force in the flow direction D of the fluid, and having a locking mechanism 40 for releasably locking the coupling 10 to a tank nipple 50, and a securing mechanism 60 for releasably blocking the closed valve 30, wherein the locking mechanism 40 and the securing mechanism 60 are coupled in terms of movement via a sliding sleeve 70 which is pre-tensioned by spring force against the flow direction D of the fluid, so that in a first control position A of the sliding sleeve 70, the locking mechanism 40 is unlocked and the valve 30 is closed, and in a second control position B of the sliding sleeve 70, the securing mechanism 60 unlocks the valve sleeve 31, so that the valve 30 is unblocked and at least partially opened, and the valve sleeve 31 can be moved subject to pressure against the flow direction D of the fluid,

Abstract: The invention relates to a coupling 10 for transferring fluids, having a valve 30, the valve tappet 32 of which is coupled in terms of movement with a valve sleeve 31, which is pre-tensioned by spring force in the flow direction D of the fluid, and having a locking mechanism 40 for releasably locking the coupling 10 to a tank nipple 50, and a securing mechanism 60 for releasably blocking the closed valve 30, wherein the locking mechanism 40 and the securing mechanism 60 are coupled in terms of movement via a sliding sleeve 70 which is pre-tensioned by spring force against the flow direction D of the fluid, so that in a first control position A of the sliding sleeve 70, the locking mechanism 40 is unlocked and the valve 30 is closed, and in a second control position B of the sliding sleeve 70, the securing mechanism 60 unlocks the valve sleeve 31, so that the valve 30 is unblocked and at least partially opened, and the valve sleeve 31 can be moved subject to pressure against the flow direction D of the fluid,

Abstract: A method of testing an agent for its potential at modulating induction of a molecular weight change of a first polyion is disclosed. The method is effected by (a) subjecting the first polyion to conditions under-which the first polyion undergoing the molecular weight change in a presence, in an absence or under several different concentrations of the agent; (b) interacting the first polyion with a second polyion having an opposite charge, the second polyion being fluorescently labeled; (c) providing reaction conditions so as to allow molecular weight discriminative interaction between the first polyion and the second polyion; and (d) employing a fluorescence polarization assay for determining a modulating effect of the agent on the induction of the molecular weight change of the first polyion.

Abstract: A method of testing an agent for its potential at modulating induction of a molecular weight change of a first polyion is disclosed. The method is effected by (a) subjecting the first polyion to conditions under-which the first polyion undergoing the molecular weight change in a presence, in an absence or under several different concentrations of the agent; (b) interacting the first polyion with a second polyion having an opposite charge, the second polyion being fluorescently labeled; (c) providing reaction conditions so as to allow molecular weight discriminative interaction between the first polyion and the second polyion; and (d) employing a fluorescence polarization assay for determining a modulating effect of the agent on the induction of the molecular weight change of the first polyion.