Patents by Inventor Rashmi Rohit Kshirsagar

Rashmi Rohit Kshirsagar has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Mammalian cell culture-produced neublastin antibodies
    Patent number: 10717965
    Abstract: The present disclosure pertains to a mammalian cell culture genetically modified to express, and which expresses, a neublastin antibody polypeptide, or fragment thereof, in the culture, and to a neublastin antibody polypeptide, or fragment thereof, made by a mammalian cell culture genetically modified to express, and which expresses, the neublastin antibody polypeptide, or fragment thereof.
    Type: Grant
    Filed: December 21, 2018
    Date of Patent: July 21, 2020
    Assignee: Gloriana Therapeutics, Inc.
    Inventors: Alan Gilbert, Kyle McElearney, Terrence Michael Dobrowsky, Rashmi Rohit Kshirsagar
  • Mammalian cell culture-produced neublastin antibodies
    Patent number: 10669523
    Abstract: The present disclosure pertains to a mammalian cell culture genetically modified to express, and which expresses, a neublastin antibody polypeptide, or fragment thereof, in the culture, and to a neublastin antibody polypeptide, or fragment thereof, made by a mammalian cell culture genetically modified to express, and which expresses, the neublastin antibody polypeptide, or fragment thereof.
    Type: Grant
    Filed: December 21, 2018
    Date of Patent: June 2, 2020
    Assignee: Gloriana Therapeutics, Inc.
    Inventors: Alan Gilbert, Kyle McElearney, Terrence Michael Dobrowsky, Rashmi Rohit Kshirsagar
  • Methods of preventing and removing trisulfide bonds
    Patent number: 10590454
    Abstract: The present invention pertains to methods of preventing and eliminating trisulfide bonds in proteins such as antibodies. In one embodiment, trisulfide bonds in proteins are converted to disulfide bonds as part of chromatographic purification procedures. In another embodiment, the formation of trisulfide bonds in proteins is inhibited by implementation of methods described herein during the cell culture production of such proteins. In another embodiment, monoclonal antibodies are produced by the methods described herein.
    Type: Grant
    Filed: October 16, 2017
    Date of Patent: March 17, 2020
    Assignee: Biogen MA Inc.
    Inventors: Rashmi Rohit Kshirsagar, Alan Gilbert
  • MAMMALIAN CELL CULTURE-PRODUCED NEUBLASTIN ANTIBODIES
    Publication number: 20190194602
    Abstract: The present disclosure pertains to a mammalian cell culture genetically modified to express, and which expresses, a neublastin antibody polypeptide, or fragment thereof, in the culture, and to a neublastin antibody polypeptide, or fragment thereof, made by a mammalian cell culture genetically modified to express, and which expresses, the neublastin antibody polypeptide, or fragment thereof.
    Type: Application
    Filed: December 21, 2018
    Publication date: June 27, 2019
    Inventors: Alan Gilbert, Kyle McElearney, Terrence Michael Dobrowsky, Rashmi Rohit Kshirsagar
  • Methods of preventing and removing trisulfide bonds
    Patent number: 10308706
    Abstract: The present invention pertains to methods of preventing and eliminating trisulfide bonds in proteins such as antibodies. In one embodiment, trisulfide bonds in proteins are converted to disulfide bonds as part of chromatographic purification procedures. In another embodiment, the formation of trisulfide bonds in proteins is inhibited by implementation of methods described herein during the cell culture production of such proteins. In another embodiment, monoclonal antibodies are produced by the methods described herein.
    Type: Grant
    Filed: March 4, 2015
    Date of Patent: June 4, 2019
    Assignee: Biogen MA Inc.
    Inventors: David Evans, R. Blake Pepinsky, Dingyi Wen, Rashmi Rohit Kshirsagar, Karin Lucas
  • METHODS OF PREVENTING AND REMOVING TRISULFIDE BONDS
    Publication number: 20180251805
    Abstract: The present invention pertains to methods of preventing and eliminating trisulfide bonds in proteins such as antibodies. In one embodiment, trisulfide bonds in proteins are converted to disulfide bonds as part of chromatographic purification procedures. In another embodiment, the formation of trisulfide bonds in proteins is inhibited by implementation of methods described herein during the cell culture production of such proteins. In another embodiment, monoclonal antibodies are produced by the methods described herein.
    Type: Application
    Filed: October 16, 2017
    Publication date: September 6, 2018
    Applicant: Biogen MA Inc.
    Inventors: Rashmi Rohit Kshirsagar, Alan Gilbert
  • Methods of preventing and removing trisulfide bonds
    Patent number: 9790533
    Abstract: The present invention pertains to methods of preventing and eliminating trisulfide bonds in proteins such as antibodies. In one embodiment, trisulfide bonds in proteins are converted to disulfide bonds as part of chromatographic purification procedures. In another embodiment, the formation of trisulfide bonds in proteins is inhibited by implementation of methods described herein during the cell culture production of such proteins. In another embodiment, monoclonal antibodies are produced by the methods described herein.
    Type: Grant
    Filed: February 6, 2017
    Date of Patent: October 17, 2017
    Assignee: Biogen MA Inc.
    Inventors: Rashmi Rohit Kshirsagar, Alan Gilbert
  • METHODS OF PREVENTING AND REMOVING TRISULFIDE BONDS
    Publication number: 20170247655
    Abstract: The present invention pertains to methods of preventing and eliminating trisulfide bonds in proteins such as antibodies. In one embodiment, trisulfide bonds in proteins are converted to disulfide bonds as part of chromatographic purification procedures. In another embodiment, the formation of trisulfide bonds in proteins is inhibited by implementation of methods described herein during the cell culture production of such proteins. In another embodiment, monoclonal antibodies are produced by the methods described herein.
    Type: Application
    Filed: February 6, 2017
    Publication date: August 31, 2017
    Inventors: Rashmi Rohit KSHIRSAGAR, Alan Gilbert
  • Methods of preventing and removing trisulfide bonds
    Patent number: 9562252
    Abstract: The present invention pertains to methods of preventing and eliminating trisulfide bonds in proteins such as antibodies. In one embodiment, trisulfide bonds in proteins are converted to disulfide bonds as part of chromatographic purification procedures. In another embodiment, the formation of trisulfide bonds in proteins is inhibited by implementation of methods described herein during the cell culture production of such proteins. In another embodiment, monoclonal antibodies are produced by the methods described herein.
    Type: Grant
    Filed: May 11, 2012
    Date of Patent: February 7, 2017
    Assignee: Biogen MA Inc.
    Inventors: Rashmi Rohit Kshirsagar, Alan Gilbert
  • MEDIUM SUPPLEMENTS FOR IMPROVED PROCESS PERFORMANCE
    Publication number: 20170029859
    Abstract: The present invention pertains to a cell culture medium comprising dextran sulfate or a mixture of dextran sulfate and ferric citrate, and methods of using thereof. The present invention further pertains to a method of producing a protein of interest in a large scale cell culture, comprising supplementing the cell culture with dextran sulfate or a mixture of dextran sulfate and ferric citrate.
    Type: Application
    Filed: October 12, 2016
    Publication date: February 2, 2017
    Inventors: Alan Gilbert, Kyle McElearney, Terrence Michael Dobrowsky, Rashmi Rohit Kshirsagar
  • Medium Supplements for Improved Process Performance
    Publication number: 20150353883
    Abstract: The present invention pertains to a cell culture medium comprising dextran sulfate or a mixture of dextran sulfate and ferric citrate, and methods of using thereof. The present invention further pertains to a method of producing a protein of interest in a large scale cell culture, comprising supplementing the cell culture with dextran sulfate or a mixture of dextran sulfate and ferric citrate.
    Type: Application
    Filed: January 10, 2014
    Publication date: December 10, 2015
    Inventors: Alan GILBERT, Kyle MCELEARNEY, Terrence Michael DOBROWSKY, Rashmi Rohit KSHIRSAGAR
  • Methods of Preventing and Removing Trisulfide Bonds
    Publication number: 20150274808
    Abstract: The present invention pertains to methods of preventing and eliminating trisulfide bonds in proteins such as antibodies. In one embodiment, trisulfide bonds in proteins are converted to disulfide bonds as part of chromatographic purification procedures. In another embodiment, the formation of trisulfide bonds in proteins is inhibited by implementation of methods described herein during the cell culture production of such proteins. In another embodiment, monoclonal antibodies are produced by the methods described herein.
    Type: Application
    Filed: March 4, 2015
    Publication date: October 1, 2015
    Inventors: David Evans, R. Blake Pepinsky, Dingyi Wen, Rashmi Rohit Kshirsagar, Karin Lucas
  • Methods of preventing and removing trisulfide bonds
    Patent number: 9005926
    Abstract: The present invention pertains to methods of preventing and eliminating trisulfide bonds in proteins such as antibodies. In one embodiment, trisulfide bonds in proteins are converted to disulfide bonds as part of chromatographic purification procedures. In another embodiment, the formation of trisulfide bonds in proteins is inhibited by implementation of methods described herein during the cell culture production of such proteins. In another embodiment, monoclonal antibodies are produced by the methods described herein.
    Type: Grant
    Filed: October 1, 2010
    Date of Patent: April 14, 2015
    Assignee: Biogen Idec Ma Inc.
    Inventors: David Evans, R. Blake Pepinsky, Dingyi Wen, Rashmi Rohit Kshirsagar, Karin Lucas
  • Methods of Preventing and Removing Trisulfide Bonds
    Publication number: 20120264916
    Abstract: The present invention pertains to methods of preventing and eliminating trisulfide bonds in proteins such as antibodies. In one embodiment, trisulfide bonds in proteins are converted to disulfide bonds as part of chromatographic purification procedures. In another embodiment, the formation of trisulfide bonds in proteins is inhibited by implementation of methods described herein during the cell culture production of such proteins. In another embodiment, monoclonal antibodies are produced by the methods described herein.
    Type: Application
    Filed: October 1, 2010
    Publication date: October 18, 2012
    Applicant: Biogen Idec MA Inc.
    Inventors: David Evans, R. Blake Pepinsky, Dingyi Wen, Rashmi Rohit Kshirsagar, Karin Lucas