Abstract: The invention relates to a polypeptide comprising an exoglucanase catalytic domain comprising a sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2 and SEQ ID NO: 3, and to a polypeptide having beta-glucosidase activity comprising a sequence selected from the group consisting of SEQ ID NO: 6, SEQ ID NO: 7 and SEQ ID NO: 8, and to functionally equivalent variants thereof that maintain or improve their catalytic activity. Additionally, the invention relates to an enzyme cocktail comprising said polypeptide(s) and an endoglucanase.

Abstract: The invention relates to an endocellulase catalytic domain comprising the sequence of SEQ ID NO: 1 or a functionally equivalent variant of said catalytic domain that substantially maintains or improves its catalytic activity. The invention also relates to a polypeptide, a nucleic acid, an expression cassette, a vector or a host cell. Additionally, the invention relates to the use of an endocellulase catalytic domain or the polypeptide of the invention for hydrolysing cellulose, producing bioethanol or as a detergent. The invention also relates to a method for hydrolysing cellulose and for producing bioethanol.

Abstract: The invention relates to a polypeptide comprising an exoglucanase catalytic domain comprising a sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2 and SEQ ID NO: 3, and to a polypeptide having beta-glucosidase activity comprising a sequence selected from the group consisting of SEQ ID NO: 6, SEQ ID NO: 7 and SEQ ID NO: 8, and to functionally equivalent variants thereof that maintain or improve their catalytic activity. Additionally, the invention relates to an enzyme cocktail comprising said polypeptide(s) and an endoglucanase.

Abstract: The invention relates to an endocellulase catalytic domain comprising the sequence of SEQ ID NO: 1 or a functionally equivalent variant of said catalytic domain that substantially maintains or improves its catalytic activity. The invention also relates to a polypeptide, a nucleic acid, an expression cassette, a vector or a host cell. Additionally, the invention relates to the use of an endocellulase catalytic domain or the polypeptide of the invention for hydrolysing cellulose, producing bioethanol or as a detergent. The invention also relates to a method for hydrolysing cellulose and for producing bioethanol.

Abstract: The disclosed subject matter relates to a force-clamp spectrometer that enables operation in constant force mode and allows for automated data acquisition and analysis, using feedback electronics and software. The disclosed subject matter also relates to methods of using the force-clamp spectrometer for the measurement of the dynamics of chemical reactions. The methods may include, but are not limited to, the measurement of the dynamics of substrate folding and unfolding, as well as bond cleavage and bond formation.

Abstract: The invention provides a method tar increasing the stability and/or activity of a polypeptide at low pH and/or elevated temperatures The invention farther provides a method for increasing the melting temperature of a polypeptide. Also provided are paleoenzymologically reconstructed thioredoxin polypeptides having activity at higher temperatures and/or lower pH than extant thioredoxin polypeptides, as well as paleoenzymologically reconstructed thioredoxin polypeptides having higher melting temperatures than extant thioredoxin polypepetides.

Abstract: The invention provides a method for increasing the stability and/or activity of a polypeptide at low pH and/or elevated temperatures. The invention further provides a method for increasing the melting temperature of a polypeptide. Also provided are paleoenzymologically reconstructed thioredoxin polypeptides having activity at higher temperatures and/or lower pH than extant thioredoxin polypepetides, as well as paleoenzymologically reconstructed thioredoxin polypeptides having higher melting temperatures than extant thioredoxin polypepetides.