Patents by Inventor Ravi David Garcia

Ravi David Garcia has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Engineered protease variants
    Patent number: 11959108
    Abstract: The present invention provides engineered protease polypeptides and compositions thereof. The engineered protease polypeptides have been optimized to provide improved activity, improved thermostability, protease stability, autolytic stability, and stability under a range of pH conditions, including acidic (pH<7) and basic (pH>7) conditions. The invention also relates to the use of the compositions comprising the engineered protease polypeptides for therapeutic and/or nutritional purposes. The present invention also provides polynucleotides encoding the engineered protease polypeptides, as well as methods for making the engineered polynucleotides and protease polypeptides.
    Type: Grant
    Filed: August 27, 2021
    Date of Patent: April 16, 2024
    Assignees: Codexis, Inc., Societe des Produits Nestle S.A.
    Inventors: Chinping Chng, Nikki Dellas, Ravi David Garcia, Moulay Hicham Alaoui Ismaili, Kristen Jean Vallieu, Kerryn McCluskie
  • ENGINEERED AMYLASE VARIANTS
    Publication number: 20240026326
    Abstract: The present invention provides engineered amylase polypeptides and compositions thereof. The engineered amylase polypeptides have been optimized to provide improved thermostability, protease stability, and stability under a range of pH conditions, including acidic (pH <7) conditions. The invention also relates to the use of the compositions comprising the engineered amylase polypeptides for therapeutic and/or nutritional purposes. The present invention also provides polynucleotides encoding the engineered amylase polypeptides, as well as methods for making the engineered polynucleotides and amylase polypeptides.
    Type: Application
    Filed: September 6, 2023
    Publication date: January 25, 2024
    Inventors: Chinping Chng, Nikki Dellas, Da Duan, Ravi David Garcia, Harvinder Chagger Maniar
  • Engineered amylase variants
    Patent number: 11767519
    Abstract: The present invention provides engineered amylase polypeptides and compositions thereof. The engineered amylase polypeptides have been optimized to provide improved thermostability, protease stability, and stability under a range of pH conditions, including acidic (pH<7) conditions. The invention also relates to the use of the compositions comprising the engineered amylase polypeptides for therapeutic and/or nutritional purposes. The present invention also provides polynucleotides encoding the engineered amylase polypeptides, as well as methods for making the engineered polynucleotides and amylase polypeptides.
    Type: Grant
    Filed: August 27, 2021
    Date of Patent: September 26, 2023
    Assignees: Codexis, Inc., Societe des Produits Nestle S.A.
    Inventors: Chinping Chng, Nikki Dellas, Da Duan, Ravi David Garcia, Harvinder Chagger Maniar
  • BIOCATALYSTS AND METHODS FOR HYDROXYLATION OF CHEMICAL COMPOUNDS
    Publication number: 20230272354
    Abstract: The present invention provides engineered proline hydroxylase polypeptides for the production of hydroxylated compounds, polynucleotides encoding the engineered proline hydroxylases, host cells capable of expressing the engineered proline hydroxylases, and methods of using the engineered proline hydroxylases to prepare compounds useful in the production of active pharmaceutical agents.
    Type: Application
    Filed: March 8, 2023
    Publication date: August 31, 2023
    Inventors: Jovana Nazor, Robert Osborne, Jack Liang, Jonathan Vroom, Xiyun Zhang, David Entwistle, Rama Voladri, Ravi David Garcia, Jeffrey C. Moore, Shane Grosser, Birgit Kosjek, Matthew Truppo
  • Vectors for expression of biocatalysts
    Patent number: 11634695
    Abstract: The present invention provides engineered proline hydroxylase polypeptides for the production of hydroxylated compounds, polynucleotides encoding the engineered proline hydroxylases, host cells capable of expressing the engineered proline hydroxylases, and methods of using the engineered proline hydroxylases to prepare compounds useful in the production of active pharmaceutical agents.
    Type: Grant
    Filed: October 16, 2020
    Date of Patent: April 25, 2023
    Assignee: Codexis, Inc.
    Inventors: Jovana Nazor, Robert Osborne, Jack Liang, Jonathan Vroom, Xiyun Zhang, David Entwistle, Rama Voladri, Ravi David Garcia, Jeffrey C. Moore, Shane Grosser, Birgit Kosjek, Matthew Truppo
  • ENGINEERED TRANSAMINASE POLYPEPTIDES FOR INDUSTRIAL BIOCATALYSIS
    Publication number: 20220282229
    Abstract: The present invention provides engineered transaminase polypeptides useful for the synthesis of chiral amine compounds under industrially relevant conditions. The invention also provides polynucleotides encoding the engineered transaminase polypeptides, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases for the production of chiral amine compounds.
    Type: Application
    Filed: May 12, 2022
    Publication date: September 8, 2022
    Inventors: Scott J. Novick, Nikki Dellas, Oscar Alvizo, Ravi David Garcia, Charlene Ching, David Entwistle
  • ENGINEERED IMINE REDUCTASES AND METHODS FOR THE REDUCTIVE AMINATION OF KETONE AND AMINE COMPOUNDS
    Publication number: 20220213518
    Abstract: The present application provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered polypeptides, host cells capable of expressing the engineered polypeptides, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.
    Type: Application
    Filed: April 23, 2020
    Publication date: July 7, 2022
    Inventors: Xiang Yi, Oscar Alvizo, Ravi David Garcia, David Entwistle, Charlene Ching, James Nicholas Riggins
  • ENGINEERED BETA-GLUCOSIDASES AND GLUCOSYLATION METHODS
    Publication number: 20220195411
    Abstract: The present invention provides engineered ?-glucosidase (BGL) enzymes, polypeptides having BGL activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention also provides compositions comprising the enzymes, and methods of using the engineered BGL enzymes to make products with ?-glucose linkages.
    Type: Application
    Filed: March 7, 2022
    Publication date: June 23, 2022
    Inventors: Jack Liang, Jonathan Vroom, Stephanie Sue Galanie, Nikki Dellas, Ravi David Garcia
  • Engineered transaminase polypeptides for industrial biocatalysis
    Patent number: 11359183
    Abstract: The present invention provides engineered transaminase polypeptides useful for the synthesis of chiral amine compounds under industrially relevant conditions. The invention also provides polynucleotides encoding the engineered transaminase polypeptides, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases for the production of chiral amine compounds.
    Type: Grant
    Filed: May 23, 2018
    Date of Patent: June 14, 2022
    Assignee: Codexis, Inc.
    Inventors: Scott J. Novick, Nikki Dellas, Oscar Alvizo, Ravi David Garcia, Charlene Ching, David Entwistle
  • PENICILLIN-G ACYLASES
    Publication number: 20220154164
    Abstract: The present invention provides engineered penicillin G acylase (PGA) enzymes having improved properties, polynucleotides encoding such enzymes, compositions including the enzymes, and methods of using the enzymes.
    Type: Application
    Filed: November 30, 2021
    Publication date: May 19, 2022
    Inventors: Jovana Nazor, Vesna Mitchell, David Elgart, Katrina W. Lexa, Nikki Dellas, Robert Kevin Orr, Oscar Alvizo, Ravi David Garcia, Judy Victoria Antonio Viduya, Courtney Dianne Moffett
  • ENGINEERED GLUCOSE DEHYDROGENASES AND METHODS FOR THE REDUCTIVE AMINATION OF KETONE AND AMINE COMPOUNDS
    Publication number: 20220145268
    Abstract: The present application provides engineered glucose dehydrogenase polypeptides having imine reductase activity, polynucleotides encoding the engineered polypeptides, host cells capable of expressing the engineered polypeptides, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.
    Type: Application
    Filed: April 23, 2020
    Publication date: May 12, 2022
    Inventors: Xiang Yi, Oscar Alvizo, Ravi David Garcia, David Entwistle, Charlene Ching, James Nicholas Riggins, Nandhitha Subramanian
  • Engineered beta-glucosidases and glucosylation methods
    Patent number: 11299723
    Abstract: The present invention provides engineered ?-glucosidase (BGL) enzymes, polypeptides having BGL activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention also provides compositions comprising the enzymes, and methods of using the engineered BGL enzymes to make products with ?-glucose linkages.
    Type: Grant
    Filed: June 9, 2017
    Date of Patent: April 12, 2022
    Assignee: Codexis, Inc.
    Inventors: Jack Liang, Jonathan Vroom, Stephanie Sue Galanie, Nikki Dellas, Ravi David Garcia
  • ENGINEERED AMYLASE VARIANTS
    Publication number: 20220090038
    Abstract: The present invention provides engineered amylase polypeptides and compositions thereof. The engineered amylase polypeptides have been optimized to provide improved thermostability, protease stability, and stability under a range of pH conditions, including acidic (pH <7) conditions. The invention also relates to the use of the compositions comprising the engineered amylase polypeptides for therapeutic and/or nutritional purposes. The present invention also provides polynucleotides encoding the engineered amylase polypeptides, as well as methods for making the engineered polynucleotides and amylase polypeptides.
    Type: Application
    Filed: August 27, 2021
    Publication date: March 24, 2022
    Inventors: Chinping Chng, Nikki Dellas, Da Duan, Ravi David Garcia, Harvinder Chagger Maniar
  • ENGINEERED PROTEASE VARIANTS
    Publication number: 20220090039
    Abstract: The present invention provides engineered protease polypeptides and compositions thereof. The engineered protease polypeptides have been optimized to provide improved activity, improved thermostability, protease stability, autolytic stability, and stability under a range of pH conditions, including acidic (pH<7) and basic (pH>7) conditions. The invention also relates to the use of the compositions comprising the engineered protease polypeptides for therapeutic and/or nutritional purposes. The present invention also provides polynucleotides encoding the engineered protease polypeptides, as well as methods for making the engineered polynucleotides and protease polypeptides.
    Type: Application
    Filed: August 27, 2021
    Publication date: March 24, 2022
    Inventors: Chinping Chng, Nikki Dellas, Ravi David Garcia, Moulay Hicham Alaoui Ismaili, Kristen Jean Vallieu, Kerryn McCluskie
  • ENGINEERED PHENYLALANINE AMMONIA LYASE POLYPEPTIDES
    Publication number: 20220056431
    Abstract: The present invention provides engineered phenylalanine ammonia lyase (PAL) polypeptides and compositions thereof, as well as polynucleotides encoding the engineered phenylalanine ammonia lyase (PAL) polypeptides. Methods for producing PAL enzymes are also provided. In some embodiments, the engineered PAL polypeptides are optimized to provide enhanced catalytic activities that are useful under industrial process conditions for the production of pharmaceutical compounds.
    Type: Application
    Filed: November 9, 2021
    Publication date: February 24, 2022
    Inventors: Scott J. Novick, Ravi David Garcia, Charlene Ching, Nikki Dellas, David Entwistle, Vesna Mitchell
  • Penicillin-G acylases
    Patent number: 11214786
    Abstract: The present invention provides engineered penicillin G acylase (PGA) enzymes having improved properties, polynucleotides encoding such enzymes, compositions including the enzymes, and methods of using the enzymes.
    Type: Grant
    Filed: June 15, 2020
    Date of Patent: January 4, 2022
    Assignee: Codexis, Inc.
    Inventors: Jovana Nazor, Vesna Mitchell, David Elgart, Katrina W. Lexa, Nikki Dellas, Robert Kevin Orr, Oscar Alvizo, Ravi David Garcia, Judy Victoria Antonio Viduya, Courtney Dianne Moffett
  • Engineered phenylalanine ammonia lyase polypeptides
    Patent number: 11198861
    Abstract: The present invention provides engineered phenylalanine ammonia lyase (PAL) polypeptides and compositions thereof, as well as polynucleotides encoding the engineered phenylalanine ammonia lyase (PAL) polypeptides. Methods for producing PAL enzymes are also provided. In some embodiments, the engineered PAL polypeptides are optimized to provide enhanced catalytic activities that are useful under industrial process conditions for the production of pharmaceutical compounds.
    Type: Grant
    Filed: June 14, 2019
    Date of Patent: December 14, 2021
    Assignee: Codexis, Inc.
    Inventors: Scott J. Novick, Ravi David Garcia, Charlene Ching, Nikki Dellas, David Entwistle, Vesna Mitchell
  • BIOCATALYSTS AND METHODS FOR HYDROXYLATION OF CHEMICAL COMPOUNDS
    Publication number: 20210032605
    Abstract: The present invention provides engineered proline hydroxylase polypeptides for the production of hydroxylated compounds, polynucleotides encoding the engineered proline hydroxylases, host cells capable of expressing the engineered proline hydroxylases, and methods of using the engineered proline hydroxylases to prepare compounds useful in the production of active pharmaceutical agents.
    Type: Application
    Filed: October 16, 2020
    Publication date: February 4, 2021
    Inventors: Jovana Nazor, Robert Osborne, Jack Liang, Jonathan Vroom, Xiyun Zhang, David Entwistle, Rama Voladri, Ravi David Garcia, Jeffrey C. Moore, Shane Grosser, Birgit Kosjek, Matthew Truppo
  • Polynucleotides encoding biocatalysts and methods for hydroxylation of chemical compounds
    Patent number: 10844358
    Abstract: The present invention provides engineered proline hydroxylase polypeptides for the production of hydroxylated compounds, polynucleotides encoding the engineered proline hydroxylases, host cells capable of expressing the engineered proline hydroxylases, and methods of using the engineered proline hydroxylases to prepare compounds useful in the production of active pharmaceutical agents.
    Type: Grant
    Filed: November 20, 2018
    Date of Patent: November 24, 2020
    Assignee: Codexis, Inc.
    Inventors: Jovana Nazor, Robert Osborne, Jack Liang, Jonathan Vroom, Xiyun Zhang, David Entwistle, Rama Voladri, Ravi David Garcia, Jeffrey C. Moore, Shane Grosser, Birgit Kosjek, Matthew Truppo
  • PENICILLIN-G ACYLASES
    Publication number: 20200339972
    Abstract: The present invention provides engineered penicillin G acylase (PGA) enzymes having improved properties, polynucleotides encoding such enzymes, compositions including the enzymes, and methods of using the enzymes.
    Type: Application
    Filed: June 15, 2020
    Publication date: October 29, 2020
    Inventors: Jovana Nazor, Vesna Mitchell, David Elgart, Katrina W. Lexa, Nikki Dellas, Robert Kevin Orr, Oscar Alvizo, Ravi David Garcia, Judy Victoria Antonio Viduya, Courtney Dianne Moffett