Patents by Inventor Ravindra Kodihalli

Ravindra Kodihalli has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Compositions and multiplex assays for characterizing active proteases and their inhibitors
    Patent number: 11408885
    Abstract: Proteases regulate a wide range of normal cellular functions where dysregulated activity is observed in various diseases. Compositions and methods use protease activity multiplexed bead-based immunoassays to profile protease activity. This platform technology integrates protease activity measurements with total protein quantification techniques. It represents a significant improvement over existing detection techniques by allowing for multiplexed, sensitive active protease measurements in complex biological samples. Exemplary multiplexed detections are realized in a single assay using a minute sample amount (e.g., 5 ?l) for active recombinant MMP-1, -2, -3, -7, 9, and 12 and those same MMPs in cell culture supernatant, menstrual fluid effluent, and peritoneal aspirates. This multiplexed platform achieves high level of sensitivities equal to or better than existing leading single-plex detection strategies.
    Type: Grant
    Filed: August 28, 2018
    Date of Patent: August 9, 2022
    Assignee: Massachusetts Institute of Technology
    Inventors: Caroline Chopko Ahrens, Linda G. Griffith, Steven Robert Tannenbaum, Christi Dionne Cook, Ravindra Kodihalli, Douglas A. Lauffenburger, Evan L. Chiswick, Miles Miller
  • COMPOSITIONS AND MULTIPLEX ASSAYS FOR CHARACTERIZING ACTIVE PROTEASES AND THEIR INHIBITORS
    Publication number: 20190064167
    Abstract: Proteases regulate a wide range of normal cellular functions where dysregulated activity is observed in various diseases. Compositions and methods use protease activity multiplexed bead-based immunoassays to profile protease activity. This platform technology integrates protease activity measurements with total protein quantification techniques. It represents a significant improvement over existing detection techniques by allowing for multiplexed, sensitive active protease measurements in complex biological samples. Exemplary multiplexed detections are realized in a single assay using a minute sample amount (e.g., 5 ?l) for active recombinant MMP-1, -2, -3, -7, 9, and 12 and those same MMPs in cell culture supernatant, menstrual fluid effluent, and peritoneal aspirates. This multiplexed platform achieves high level of sensitivities equal to or better than existing leading single-plex detection strategies.
    Type: Application
    Filed: August 28, 2018
    Publication date: February 28, 2019
    Inventors: Caroline Chopko Ahrens, Linda G. Griffith, Steven Robert Tannenbaum, Christi Dionne Cook, Ravindra Kodihalli, Douglas A. Lauffenburger, Evan L. Chiswick, Miles Miller