Patents by Inventor Ray A. McMillian
Ray A. McMillian has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11913083Abstract: A real time Taq-Man PCR assay for detecting multiple serotypes of human papillomavirus (HPV) wherein the number of serotypes detected exceeds the number of colorimetric channels for detection. A biological sample is combined with three oligonucleotide primer/probe sets such that the probes and primers anneal to a target sequence. Each primer/probe set is at least preferential for a specific serotype of an organism. The first and second primer/probe sets are degenerate with respect to each other. The third primer/probe set is not degenerate with respect to the first and second primer/probe sets and discriminates for a third serotype. The third primer/probe set has a signal moiety that emits signal at a wavelength that is the same or different from the wavelength emitted by the signal moiety of the degenerate primer/probe set probes. The target sequences, if present, are amplified and detected.Type: GrantFiled: November 11, 2020Date of Patent: February 27, 2024Assignee: Becton, Dickinson and CompanyInventors: Chi Chen, Hugh J. Peck, Michael Porter, Gregory A. Richart, Ray A. McMillian
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Patent number: 11773457Abstract: A region of the Trichomonas vaginalis AP65-1 gene has been identified which is useful for performing amplification assays to determine specifically whether T. vaginalis is present in the sample being tested. Oligonucleotides useful for performing thermal Strand Displacement Assay (tSDA) reactions on this gene are disclosed. The disclosed oligonucleotides can be used in an assay which is specific for multiple strains of T. vaginalis and which does not show cross reactivity with the genomes of other microorganisms or with human DNA.Type: GrantFiled: August 21, 2021Date of Patent: October 3, 2023Assignee: BECTON, DICKINSON AND COMPANYInventor: Ray A. McMillian
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Patent number: 11124844Abstract: A region of the Trichomonas vaginalis AP65-1 gene has been identified which is useful for performing amplification assays to determine specifically whether T. vaginalis is present in the sample being tested. Oligonucleotides useful for performing thermal Strand Displacement Assay (tSDA) reactions on this gene are disclosed. The disclosed oligonucleotides can be used in an assay which is specific for multiple strains of T. vaginalis and which does not show cross reactivity with the genomes of other microorganisms or with human DNA.Type: GrantFiled: May 2, 2018Date of Patent: September 21, 2021Assignee: BECTON, DICKINSON AND COMPANYInventor: Ray A. McMillian
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Patent number: 10865455Abstract: A real time Taq-Man PCR assay for detecting multiple serotypes of human papillomavirus (HPV) wherein the number of serotypes detected exceeds the number of colorimetric channels for detection. A biological sample is combined with three oligonucleotide primer/probe sets such that the probes and primers anneal to a target sequence. Each primer/probe set is at least preferential for a specific serotype of an organism. The first and second primer/probe sets are degenerate with respect to each other. The third primer/probe set is not degenerate with respect to the first and second primer/probe sets and discriminates for a third serotype. The third primer/probe set has a signal moiety that emits signal at a wavelength that is the same or different from the wavelength emitted by the signal moiety of the degenerate primer/probe set probes. The target sequences, if present, are amplified and detected.Type: GrantFiled: December 15, 2017Date of Patent: December 15, 2020Assignee: BECTON DICKINSON AND COMPANYInventors: Chi Chen, Hugh J. Peck, Michael Porter, Gregory A. Richart, Ray A. McMillian
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Patent number: 9988692Abstract: A region of the Trichomonas vaginalis AP65-1 gene has been identified which is useful for performing amplification assays to determine specifically whether T. vaginalis is present in the sample being tested. Oligonucleotides useful for performing thermal Strand Displacement Assay (tSDA) reactions on this gene are disclosed. The disclosed oligonucleotides can be used in an assay which is specific for multiple strains of T. vaginalis and which does not show cross reactivity with the genomes of other microorganisms or with human DNA.Type: GrantFiled: December 19, 2014Date of Patent: June 5, 2018Assignee: Becton, Dickinson and CompanyInventor: Ray A. McMillian
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Publication number: 20180127839Abstract: A real time Taq-Man PCR assay for detecting multiple serotypes of human papillomavirus (HPV) wherein the number of serotypes detected exceeds the number of colorimetric channels for detection. A biological sample is combined with three oligonucleotide primer/probe sets such that the probes and primers anneal to a target sequence. Each primer/probe set is at least preferential for a specific serotype of an organism. The first and second primer/probe sets are degenerate with respect to each other. The third primer/probe set is not degenerate with respect to the first and second primer/probe sets and discriminates for a third serotype. The third primer/probe set has a signal moiety that emits signal at a wavelength that is the same or different from the wavelength emitted by the signal moiety of the degenerate primer/probe set probes. The target sequences, if present, are amplified and detected.Type: ApplicationFiled: December 15, 2017Publication date: May 10, 2018Applicant: BECTON DICKINSON AND COMPANYInventors: Chi Chen, Hugh J. Peck, Michael Porter, Gregory A. Richart, Ray A. McMillian
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Patent number: 9868997Abstract: A real time Taq-Man PCR assay for detecting multiple serotypes of human papillomavirus (HPV) wherein the number of serotypes detected exceeds the number of colorimetric channels for detection. A biological sample is combined with three oligonucleotide primer/probe sets such that the probes and primers anneal to a target sequence. Each primer/probe set is at least preferential for a specific serotype of an organism. The first and second primer/probe sets are degenerate with respect to each other. The third primer/probe set is not degenerate with respect to the first and second primer/probe sets and discriminates for a third serotype. The third primer/probe set has a signal moiety that emits signal at a wavelength that is the same or different from the wavelength emitted by the signal moiety of the degenerate primer/probe set probes. The target sequences, if present, are amplified and detected.Type: GrantFiled: February 12, 2014Date of Patent: January 16, 2018Assignee: Becton, Dickinson and CompanyInventors: Chi Chen, Hugh J. Peck, Michael Porter, Gregory A. Richart, Ray A. McMillian
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Patent number: 9777336Abstract: A region of the Chlamydia trachomatis cytotoxin gene has been identified which is useful for performing amplification assays to determine specifically whether C. trachomatis is present in the sample being tested. Oligonucleotides useful for performing thermal Strand Displacement Assay (tSDA) reactions on this gene are disclosed. The disclosed oligonucleotides can be used in an assay which is specific for multiple strains of C. trachomatis and which does not show cross reactivity with the genomes of other microorganisms or with human DNA.Type: GrantFiled: August 26, 2009Date of Patent: October 3, 2017Assignee: Becton, Dickinson and CompanyInventors: Courtney E. Maus, Ray A. McMillian
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Patent number: 8945842Abstract: A region of the Trichomonas vaginalis AP65-1 gene has been identified which is useful for performing amplification assays to determine specifically whether T. vaginalis is present in the sample being tested. Oligonucleotides useful for performing thermal Strand Displacement Assay (tSDA) reactions on this gene are disclosed. The disclosed oligonucleotides can be used in an assay which is specific for multiple strains of T. vaginalis and which does not show cross reactivity with the genomes of other microorganisms or with human DNA.Type: GrantFiled: January 13, 2010Date of Patent: February 3, 2015Assignee: Becton, Dickinson and CompanyInventor: Ray McMillian
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Patent number: 8859199Abstract: The present invention relates to a method of ensuring the effectiveness of the extraction workup from a biological sample of nucleic acid. The inventive method is able to distinguish between possible defects in the extraction of nucleic acid from a sample and possible defects in a subsequent amplification step. The present invention also relates to a packaged array for extracting nucleic acid from a biological sample.Type: GrantFiled: April 4, 2008Date of Patent: October 14, 2014Assignee: Becton, Dickinson and CompanyInventors: Tobin Hellyer, Thomas Fort, Ray A. McMillian
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Patent number: 8658397Abstract: A real time Taq-Man PCR assay for detecting multiple serotypes of human papillomavirus (HPV) wherein the number of serotypes detected exceeds the number of colorimetric channels for detection. A biological sample is combined with three oligonucleotide primer/probe sets such that the probes and primers anneal to a target sequence. Each primer/probe set is at least preferential for a specific serotype of an organism. The first and second primer/probe sets are degenerate with respect to each other. The third primer/probe set is not degenerate with respect to the first and second primer/probe sets and discriminates for a third serotype. The third primer/probe set has a signal moiety that emits signal at a wavelength that is the same or different from the wavelength emitted by the signal moiety of the degenerate primer/probe set probes. The target sequences, if present, are amplified and detected.Type: GrantFiled: February 16, 2011Date of Patent: February 25, 2014Assignee: Becton, Dickinson and CompanyInventors: Chi Chen, Michael Porter, Gregory A. Richart, Ray A. McMillian, Dolores C. Peck
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Publication number: 20090149337Abstract: The present invention relates to a method of ensuring the effectiveness of the extraction workup from a biological sample of nucleic acid. The inventive method is able to distinguish between possible defects in the extraction of nucleic acid from a sample and possible defects in a subsequent amplification step. The present invention also relates to a packaged array for extracting nucleic acid from a biological sample.Type: ApplicationFiled: April 4, 2008Publication date: June 11, 2009Applicant: Becton, Dickinson and CompanyInventors: Tobin Hellyer, Thomas Fort, Ray A. McMillian, Matthew P. Collis, Thomas L. Fort
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Publication number: 20080113402Abstract: A method includes providing a sample containing at least one whole, intact particle or organism in a container; creating a mixture comprising the sample, at least one magnetically-responsive particle, and a remainder; and providing the mixture with a pH of less than about 7.0. By providing the mixture with a pH of less than about 7.0, alteration of the surface charge properties of at least the one magnetic particle occurs, thereby causing the at least one whole, intact particle or organism to become non-specifically bound to the at least one magnetically-responsive particle to form a complex.Type: ApplicationFiled: January 17, 2008Publication date: May 15, 2008Applicant: BECTON DICKINSON AND COMPANYInventor: Ray McMillian
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Patent number: 7371531Abstract: The present invention relates to a method of ensuring the effectiveness of the extraction workup from a biological sample of nucleic acid. The inventive method is able to distinguish between possible defects in the extraction of nucleic acid from a sample and possible defects in a subsequent amplification step. The present invention also relates to a packaged array for extracting nucleic acid from a biological sample.Type: GrantFiled: April 25, 2005Date of Patent: May 13, 2008Assignee: Becton, Dickinson and CompanyInventors: Tobin Hellyer, Thomas Fort, Ray A. McMillian
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Patent number: 7029840Abstract: The present invention provides a method for preserving cells and nucleic acids in a sample by exposing the sample to a composition that causes an inhibitory affect on proteolytic agents and/or nucleic acid degradative agents in the sample. Such compositions that are useful in the method of the present invention include chelating agents such as sodium citrate, sodium borate, sodium fluoride and EDTA, that will bind trace metals necessary for proteolytic activity of proteolytic agents and/or nuclease activity of nuclease agents in a sample.Type: GrantFiled: August 20, 2002Date of Patent: April 18, 2006Assignee: Becton, Dickinson and CompanyInventor: Ray A. McMillian
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Publication number: 20060024776Abstract: A method includes providing a sample containing at least one whole, intact particle or organism in a container; creating a mixture comprising the sample, at least one magnetically-responsive particle, and a remainder; and providing the mixture with a pH of less than about 7.0. By providing the mixture with a pH of less than about 7.0, alteration of the surface charge properties of at least the one magnetic particle occurs, thereby causing the at least one whole, intact particle or organism to become non-specifically bound to the at least one magnetically-responsive particle to form a complex.Type: ApplicationFiled: August 2, 2004Publication date: February 2, 2006Inventor: Ray McMillian
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Publication number: 20060014170Abstract: The present invention relates to a method of ensuring the effectiveness of the extraction workup from a biological sample of nucleic acid. The inventive method is able to distinguish between possible defects in the extraction of nucleic acid from a sample and possible defects in a subsequent amplification step. The present invention also relates to a packaged array for extracting nucleic acid from a biological sample.Type: ApplicationFiled: April 25, 2005Publication date: January 19, 2006Inventors: Tobin Hellyer, Thomas Fort, Ray McMillian
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Patent number: 6586584Abstract: Primers and probes derived from the 5′ untranslated region of the HCV genome which facilitate detection and/or quantification of all presently known genotypes of HCV. Disclosed sequences may be used in a variety of primer and probe constructs for amplification and/or detection of HCV nucleic acids.Type: GrantFiled: January 29, 2001Date of Patent: July 1, 2003Assignee: Becton, Dickinson and CompanyInventors: Ray A. McMillian, Tobin J. Hellyer
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Publication number: 20030113705Abstract: The present invention provides a method for preserving cells and nucleic acids in a sample by exposing the sample to a composition that causes an inhibitory affect on proteolytic agents and/or nucleic acid degradative agents in the sample. Such compositions that are useful in the method of the present invention include chelating agents such as sodium citrate, sodium borate, sodium fluoride and EDTA, that will bind trace metals necessary for proteolytic activity of proteolytic agents and/or nuclease activity of nuclease agents in a sample.Type: ApplicationFiled: August 20, 2002Publication date: June 19, 2003Inventor: Ray A. McMillian
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Publication number: 20030032004Abstract: Primers and probes derived from the 5′ untranslated region of the HCV genome which facilitate detection and/or quantification of all presently known genotypes of HCV. Disclosed sequences may be used in a variety of primer and probe constructs for amplification and/or detection of HCV nucleic acids.Type: ApplicationFiled: January 29, 2001Publication date: February 13, 2003Inventors: Ray A. McMillian, Tobin J. Hellyer