Abstract: The present invention comprises a diagnostic platform. The platform has a substrate having plurality of hydrophobic wells formed thereon. The hydrophobic wells are directly printed onto the substrate in a test pattern and are adapted to contain a biological sample for conducting a diagnostic assay to identify analytes within the biological sample. The test pattern is divided into equally sized quadrants, each containing an equal number of hydrophobic wells. The hydrophobic wells may be in a single or double well arrangement, as well as may be connected by at least one microfluidic channel.