Abstract: A method for the acyl fluoride activation of a carboxysilyl-coated glass is described. The method includes providing a glass substrate having a surface with at least one pendant carboxysilyl linker comprising a carboxyl group and activating without etching the surface of the glass by converting the carboxyl group into an acyl fluoride group. In one embodiment, the surface of the glass may be activated by contacting the surface with a fluoridating agent and a tertiary amine reagent, and a solvent that is capable of binding hydrogen fluoride. A substrate for the covalent immobilization of nucleophile-containing species is also provided. The substrate comprises a glass having a surface with a pendant carboxysilyl linker activated by acyl fluoride without etching the surface of the glass. The invention also provides a bioarray formed on a glass surface activated with acyl fluorides without etching.

Abstract: Detection devices for multianalyte detection on a solid substrate, methods for the preparation of the devices and their use in analytical and diagnostic procedures are described. The detection devices include a solid substrate fabricated with an array of detection spots, the detection spots having an analyte sensor bound to the substrate by a universal binding ligand. The universal binding ligand is capable of binding multiple analyte sensors to create a multifunctional array. A process for producing the detection devices and assay methods employing microprinting technology are also described.

Abstract: A method for the acyl fluoride activation of a carboxysilyl-coated glass is described. The method includes providing a glass substrate having a surface with at least one pendant carboxysilyl linker comprising a carboxyl group and activating without etching the surface of the glass by converting the carboxyl group into an acyl fluoride group. In one embodiment, the surface of the glass may be activated by contacting the surface with a fluoridating agent and a tertiary amine reagent, and a solvent that is capable of binding hydrogen fluoride. A substrate for the covalent immobilization of nucleophile-containing species is also provided. The substrate comprises a glass having a surface with a pendant carboxysilyl linker activated by acyl fluoride without etching the surface of the glass. The invention also provides a bioarray formed on a glass surface activated with acyl fluorides without etching.

Abstract: This invention provides dyes, particularly cyanine and related dyes, with acyl fluoride activating groups, the dyes having the general formula wherein: each dotted line represents carbon atoms necessary to form a fused substituted or unsubstituted aromatic ring; n is an integer selected from the group consisting of 1, 2 and 3; X and Y are selected from the group consisting of S, O, N, CH2 and C(CH3)2; at least one of said R1 and R2 comprises a sulfonic acid or sulfonate group attached to the aromatic ring; and R3 and R4 are independently selected from the group consisting of alkylcarboxylate, activated alkylcarboxylate and an inert group; wherein at least one of said R3 and R4 groups is alcylcarboxylate or activated alkylcarboxylate with the carboxyl group converted to an acyl fluoride. The inert group is a group that is inert towards acyl fluoride and has a sterical structure which allows aminoacylation of the acyl fluoride group.

Abstract: A method of attaching unmodified biopolymers, particularly, unmodified polynucleotides, directly to a solid support is provided. The method includes the steps of (a) providing unmodified biopolymers; (b) providing a solid support having at least one surface comprising pendant acyl fluoride functionalities; and (c) contacting the unmodified biopolymers with the solid support under a condition sufficient for allowing the attachment of the biopolymers to the solid support. The unmodified biopolymers may be nucleic acids, polypeptides, proteins, carbohydrates, lipids and analogues thereof The unmodified polynucleotides may be DNA, RNA or synthesized oligonucleotides. The DNA may be single or double stranded. A device including a solid support and unmodified biopolymers attached to the solid support by reaction with the pendant acyl fluoride functionalities of the solid support is also provided.

Abstract: A method of attaching unmodified biopolymers, particularly, unmodified polynucleotides, directly to a solid support is provided. The method includes the steps of (a) providing unmodified biopolymers; (b) providing a solid support having at least one surface comprising pendant acyl fluoride functionalities, and (c) contacting the unmodified biopolymers with the solid support under a condition sufficient for allowing the attachment of the biopolymers to the solid support. The unmodified biopolymers may be nucleic acids, polypeptides, proteins, carbohydrates, lipids and analogues thereof. The unmodified polynucleotides may be DNA, RNA or synthesized oligonucleotides. The DNA may be single or double stranded. A device including a solid support and unmodified biopolymers attached to the solid support by reaction with the pendant acyl fluoride functionalities of the solid support is also provided. The methods and devices of the present invention may be used in performing hybridization assays and immunoassays.

Abstract: Reagents for the immobilization of biopolymers, processes for their preparation and their subsequent use in the immobilization of biopolymers for analytical and diagnostic procedures are described. One type of reagent includes a solid support fabricated of a polymeric material having at least one surface with pendant acyl fluoride functionalities. Another reagent includes solid supports fabricated of polymeric materials including ethylene acrylic acid or ethylene methacrylic acid copolymers and activated polypropylene. Processes for preparing reagents include derivatizing polymeric materials to form acyl fluoride functionalities or derivatizing ethylene acrylic acid copolymers and ethylene methacrylic acid copolymers to form active acyl functionalities.

Abstract: Reagents for the immobilization of biopolymers, processes for their preparation and their subsequent use in the immobilized of biopolymers for analytical and diagnostic procedures are described. One type of reagent includes a solid support fabricated of a polymeric material having at least one surface with pendant acyl fluoride functionalities, Another reagent includes solid supports fabricated of polymeric materials including ethylene acrylic acid or ethylene methacrylic acid copolymers and activated polypropylene. Processes for preparing reagents include derivatizing polymeric materials to form acyl fluoride functionalities or derivatizing ethylene acrylic acid copolymers and ethylene methacrylic acid copolymers to form active acyl functionalities.